Acute Toxicity, Immunotoxicity and Allergenicity of Protease Complex Obtained from Micromycete .

The different effects on animals of the thrombolytic protease complex of the new producer 203 were studied. The tests of acute toxicity, immunotoxicity and allergenicity should conclude that the studied proteolytic complex is safe for medical usage. For the intravenous and the intraperitoneal routes of administration, the maximum tolerated dose and the median lethal dose were not determined.

Optimization of triacetate cellulose hollow fiber vitrification (HFV) method for cryopreservation of in vitro matured bovine oocytes.

The aim of the current work was to evaluate applicability of triacetate cellulose hollow fiber vitrification (HFV) method for cryopreservation of groups of in vitro matured bovine oocytes (12-17 oocytes per device). We also attempted to optimize HFV protocol by altering concentration of non-permeating cryoprotectant (sucrose) in vitrification solution and concentration of extracellular Ca by using a calcium-free base medium for preparation of vitrification/rewarming solutions with ethylene glycol (EG) as a single permeating cryoprotectant. Neither of modifications of HFV protocol significantly affected survival or fertilization rates of the vitrified bovine oocytes.

[The Russian consensus on the diagnosis and treatment of chronic pancreatitis: Enzyme replacement therapy].

Pancreatology Club Professional Medical Community, 1A.S. Loginov Moscow Clinical Research and Practical Center, Moscow Healthcare Department, Moscow; 2A.

[All-Russian Consensus on Diagnosis and Treatment of Celiac Disease in Children and Adults].

The paper presents the All-Russian consensus on the diagnosis and treatment of celiac disease in children and adults, which has been elaborated by leading experts, such as gastroenterologists and pediatricians of Russia on the basis of the existing Russian and international guidelines. The consensus approved at the 42nd Annual Scientific Session of the Central Research Institute of Gastroenterology on Principles of Evidence-Based Medicine into Clinical Practice (March 2-3, 2016). The consensus is intended for practitioners engaged in the management and treatment of patients with celiac disease.

[Morphological and Physiological Properties of the Micromycete Arthrobotrys longa, a Producer of Longolytin, a Proteolytic Complex with a Thrombolytic Effect].

Production of proteinases with plasmin-like and plasminogen-activating activities by a micromycete Arthrobotrys longa 1 was studied. Polycyclic growth of the producer in submerged cultures was observed, with an endogenous rhythm of the periods of intense microconidia formation alternating with the phases of drastic increase in the content of producing mycelium. The highest plasminogen-like and plasminogen-activating activities (up to 1000 and 500 cond.

First stage larva of the mud shrimp Nihonotrypaea makarovi Marin, 2013 (Decapoda: Axiidea: Callianassidae) obtained in the laboratory.

First stage larva of a new mud shrimp Nihonotrypaea makarovi Marin, 2013 (Decapoda: Axiidea: Callianassidae) obtained from the ovigerous female is described and illustrated for the first time. The first zoea of N. makarovi is well distinguished from the first larvae of N.

[Sympathetic block as a part of the treatment of coronary heart disease (Literature review)].

The given literature review is devoted to the urgent problem of improving the effectiveness of conservative treatment of patients with coronary heart disease through the use of sympathetic blockade. The authors highlight the. current understanding of the morbid physiology of cardiac angina, in particular, pain syndrome, of the state of analgesia in cardiology.

[NONALCOHOLIC FATTY LIVER DISEASE: DIAGNOSTIC, SYMPTOMS, TREATMENT. GUIDELINES WERE APPROVED BY THE XV GASTROENTEROLOGICAL SCIENTIFIC SOCIETY OF RUSSIA IN 2015].

According to the World Health Organization, it is recorded steady growth of the number of chronic liver diseases, including nonalcoholic fatty liver disease (NAFLD), in recent years. As an independent nosological entity, NAFLD is one of the risk factors for cardiovascular diseases, associated with abdominal-visceral obesity, peripheral insulin resistance (IR), and is regarded as the hepatic component of Metabolic syndrome (MS). Nowadays there are no generally accepted national standards for diagnosis and treatment of NAFLD for physicians, gen- eral practitioners, gastroenterologists in Russia.

BASIC BIOCHEMICAL AND CLINICAL ASPECTS OF NONINVASIVE TESTS HELIC.

Biochemical process that lay in the core of non-invasive detection of Helico ho cter pylod with the help of HELIC Ammonia breath test, manufactured by AMA Co Ltd., St.Petersburg, is shown.

Zoeal stages of Labidochirus anomalus (Balss, 1913) (Decapoda: Anomura: Paguridae) obtained under laboratory conditions.

Zoeal stages of the hermit crab Labidochirus anomalus (Balss, 1913) (Decapoda: Anomura: Paguridae) are described and illustrated from the larvae reared in the laboratory. The development included four zoeal stages following the typical pattern in the Paguridae. Morphological features of the larvae of L.

The Photovoltaic Effect of CdS Quantum Dots Synthesized in Inverse Micelles and R-Phycoerythrin Tunnel Cavities.

CdS quantum dots (CdS QDs) 4.3 nm in diameter synthesized in an AOT/isooctane/water microemulsion and in R-phycoerythrin tunnel cavities (3.5 × 6.

[Osphradial chemosensory organ as a probable trigger of the cardiac system adaptive reaction to the effect of heavy metals in aquatic mollusks].

The responses of osphradium in the fresh-water mollusk Viviparus sp. and single osphradial neurons in the pond snail Lymnaea stagnalis to L-glutamine and L-asparagine as well as the changes in these responses under the effect of heavy metals (Cu, Cd) were recorded electrophysiologically. The functional connections of osphradium with the identified neurons of the central pattern generator of respiratory movements and cardiac activity as well as the modification of these connections under the effect of short-term application of HgCl2 solution on the snail's osphradium were investigated.

The number of zoeal stages in larval development of Nihonotrypaea petalura (Stimpson, 1860) (Decapoda: Axiidea: Callianassidae) from Russian waters of the Sea of Japan.

Zoeal stages of the mud shrimp Nihonotrypaea petalura (Stimpson, 1860) (Decapoda: Axiiidea: Callianassidae) inhabiting Peter the Great Bay (Sea of Japan) is described and illustrated from the larvae reared in the laboratory individually. The development included seven zoeal stages before molting to the megalopa. At 22-23°C the first megalopa was attained 26 days after hatching.

Estimation of isotonic point of incubation medium for two-cell mouse embryo.

Osmolarity of Dulbecco's medium at which the volume of two-cell mouse embryo remained similar to that of intact embryo was determined. The method is based on comparison of kinetic curves describing the volume of embryonic cell in solutions of different osmolarity. The blastomere volume was measured by quantitative laser microtomography after fixed osmotic stress intervals.

[Heterogeneity of chronic esophagitis in childhood].

Chronic esophagitis in children etiologically are heterogeneous. Was observed 83 patients aged from 3 to 17 years with histologically defined chronic esophagitis, 58 of them suffered from food and respiratory allergies. All children underwented endoscopy, morphological and immunohistochemical study of the esophagus biopsies with the definition IgE, IgA herpes simplex virus (HSV), cytomegalovirus (CMV) and Epstein - Barr virus (EBV) in the mucous membrane.

Electrochemical, ESR and theoretical studies of [6,6]-opened C(60)(CF(2)), cis-2-C(60)(CF(2))(2) and their anions.

Electrochemical behavior of C(60)(CF(2))(n), n = 1, 2 and C(60)(CCl(2)) has been investigated. [6,6]-Opened C(60)(CF(2)) and cis-2-C(60)(CF(2))(2) exhibit reversible reductions at potentials 150 and 145 mV more positive than C(60), unlike the related [6,6]-closed C(60)(CCl(2)), which shows reduction at the potential close to that for C(60). The structures, electron localization and lifetimes of the observed radical anions C(60)(CF(2))(-*) and cis-2-C(60)(CF(2))(2)(-*) have been elucidated by the ESR study and DFT calculations.

A unique seroepidemiological pattern of HBV, HCV and HTLV-I in Nenets and Komi in northwestern Russia.

An epidemiological study of hepatitis viruses type B (HBV) and type C (HCV) and human T-cell leukemia virus type I (HTLV-I) was carried out among 105 residents (male:female=19:86) regarded as Nenets partly mixed with Komi, in the region of Krasnoe, the Nenets Autonomous District of the Arkhangelsk Region, in northwestern Russia in 2004. Blood was drawn from apparently healthy volunteers at ages of 41.6+/-16.

[Results of child observation during 3 years after the eradication treatment].

Comparative evaluation of clinical, endoscopic and morphological stomach mucosa changes dynamics was performed in 100 patients with presence and absence of HP eradication during 3 years. Improvement of clinical, endoscopic and disease morphological sings, sometimes the complete its normalization, but never worsening were observed in the most patients with HP eradication. The main pathomorphological indexes were for certain decreased after 1 year of eradication.

[Various problems of small-intestinal pathology in children with food allergy].

Altogether 54 children with food allergy characterized clinically by concomitant lesion of the skin and alimentary tract were examined. The results obtained attest to the involvement into the pathological process of the small intestine, to the combination of cow's milk protein and lactose intolerance as well as to the advisability of correcting intestinal dysbiocenosis in order to reduce the disease progress.