Responses of MC3T3-E1 cells to three dental resin-based restorative materials.

This study aimed to examine the influences of three dental resin-based restorative materials on cells associated with hard tissue regeneration using osteoblastic MC3T3-E1 cells. A Bis-GMA-based resin composite [Clearfil AP-X (APX)], an MMA-based resin cement [Superbond C&B (SB)], and a resin-modified glass-ionomer [Fuji Ionomer Type II LC (LC)] were tested. A zinc oxide eugenol cement [Super EBA (EBA)] was included in the study for comparison.

Comparison of osteoblast responses to hydroxyapatite and hydroxyapatite/soluble calcium phosphate composites.

Hydroxyapatite/soluble calcium phosphate composites (HAp/SCaP) are novel HAp-based materials with enhanced solubility that have been developed by annealing HAp in a vacuum. This study compared the effects of HAp and HAp/SCaP on osteoblast proliferation, differentiation, and mineralization using an MC3T3-E1 cell culture system. MC3T3-E1 cells were cultured on HAp or HAp/SCaP, and the number of attached cells and their morphology were examined.

Effects of alpha-TCP and TetCP on MC3T3-E1 proliferation, differentiation and mineralization.

Calcium phosphates with high solubility in water such as alpha-tricalcium phosphate (alpha-TCP) and tetracalcium phosphate (TetCP) have received considerable attention as components of bone-substitution materials. However, the osteoblast response to these materials has not yet been clearly understood. This study examined the effects of alpha-TCP and TetCP on osteoblast proliferation, differentiation and mineralization in the culture system of MC3T3-E1 cells.