[The significance of individual inhalation allergens in the sensitization of children with respiratory allergy].

Four hundred children were skin tested with following allergens: Dermatophagoides pteronyssinus, moulds, animal dander (mixture of dog, cat, sheep and horse dander), tree pollens, weed pollen (Ambrosia artemisifolia), and six grass pollens, as well as three nutritive allergens in children less than three years of age. Results demonstrated that in 200 children with perenial rhinitis and/or asthma. Dermatophagoides gave positive results in over 90%.

[Chromosome abnormalities in malignant lymphoma].

Analysis of the karyotype from bone marrow tissue and lymphocyte culture of the peripheral blood was performed in 25 children either with Hodgkin's or non-Hodgkins' lymphoma prior to any treatment. numeric aberrations were confirmed in 40% of the patients; in these the hyperdiploid number of chromosomes ranged from 52 to 94, while 24% of the cases had cells with 47 chromosomes of which the surplus chromosome corresponded to those of the group C and, in one case, of the group G. The occurence of cells with abnormal chromosome sets confirmed presence of clonal evolution.

[Chemotherapy and mutagenesis. A study of chromosome aberrations as a test for mutagenesis after the use of cyclophosphamide, methotrexate and cytosine arabinoside].

Determination of chromosomal aberration in the lymphocyte cell culture of the peripheral blood in 50 children with malignant and nonmalignant diseases was the test applied in the investigation of the adverse effects of the cytostatic therapy on the human genome. The study included the cytostatic drugs cyclophosphamide, methotrexat and cytosine arabinoside which are used in the treatment for the autoimmune diseases, in organ and bone marrow transplantations and in malignant diseases. It was confirmed that these cytostatics could be the cause of considerable structural aberrations in chromosomes, particularly so when high dosages and long application are involved.

[Genetic counseling in hematologic diseases].

In patients with hereditary haematologic diseases risk of repetition of these anomalies in brothers and sisters of probands and their progenies was assessed. In haemiphilia and Wiskott-Aldrich's. thrombocytopenia, both caused by a mutant gene in an X chromosome, prenatal pole determination was consulted in order to have a pregnancy with a male fetus interrupted due to high risk.

[Blood groups in chromosome disorders].

The authors investigated blood groups of two cases of polisomia, and 6 cases of chromosomal aberations. While the polisomia demonstrated no abnormalities of the blood groups, three cases of the chromosomal aberation show an alteration on the chromosome 2 and atypical inheritance of the MNSs groups, confirming autosomal location of these blood groups.

[Congenital deficiency of humoral and cellular immunity].

The study involves 10 children with primary immunologic deficiency of the humoral and cellular immunity diagnosed on the basis of the clinical symptoms of the disease and immunologic investigation. In 7 children, out of whome three were infants, suffering of recurrent respiratory tract infections, examinations revealed either absence or deficiency of the IgA in the serum and saliva. 2 children presented a classical picture of the Wiskott-Aldrich's syndrome followed by eczema, recurrent infections and trombocytopenia.

[Cytogenetic examination of children with chronic granulocytic leukemia].

The most important marker chromosome in patients with CDL is small acrocentric chromosome which is due to the deletion one of the chromosome 22. Ph 1 can be found only in adult type of CDL. Dhe authors present their experiences in citogeneticaly examined children with CDL.