Potential link between MHC-self-peptide presentation and hematopoiesis; the analysis of HLA-DR expression in CD34-positive cells and self-peptide presentation repertoires of MHC molecules associated with paroxysmal nocturnal hemoglobinuria.

The mechanisms of MHC allele associations with paroxysmal nocturnal hemoglobinuria (PNH) and its aplastic anemia subtype (AA/PNH) remain unclear. It might be dependent on MHC molecule functional properties, such as a scope and frequency of antigen sampling and presentation. For documented PNH-associated MHC alleles we analyzed current reference databases on MHC molecule-eluted peptide presentation repertoires and searched for a range of presented peptides.

Flow cytometric immunophenotypic characteristics of plasma cell leukemia.

The aim of this prospective study was to define the flow cytometric characteristics of simultaneously investigated bone marrow and peripheral blood plasma cells antigens expression in 36 plasma cell leukemia (PCL) patients. The immunophenotypic profile of plasma cells was determined with a panel of monoclonal antibodies. The antigen expression intensity was calculated as relative fluorescence intensity (RFI).

Flow cytometric immunophenotypic characteristics of 36 cases of plasma cell leukemia.

Prospective flow cytometric analysis of antigens expression on bone marrow and peripheral blood plasma cells of 36 plasma cell leukemia (PCL) patients enabled to establish the following immunophenotype of leukemic plasma cell: CD38(++), CD138(+), CD54(+), CD49d(+), CD29(+), CD44(+), CD126(+), CD19(-), CD45(-). In one-third of patients PCL cells express CD56, CD71 and CD117. Expression of CD54 on plasma cells was higher as compared to expression of adhesion molecules CD11a, CD18 and CD11b (p<0.

Clinicopathological correlates of plasma cell CD56 (NCAM) expression in multiple myeloma.

The aim of this prospective, long-term study was to define the flow cytometric characteristics of plasma cell CD56 expression as well as determine the clinical characteristics of 204 multiple myeloma (MM) patients and 26 plasma cell leukemia (PCL) patients with regard to CD56 expression. CD56 expression intensity was determined by measurement of antigen molecules on the cell defined as Antibodies Bound per Cell (ABC) and calculation of Relative Fluorescence Intensity (RFI). CD56 expression was found in 66% of MM and 54% of PCL cases.

B cells CD19+ in patients with B-cell chronic lymphocytic leukaemia and autoimmune haemolytic anaemia.

The study presents results of B and T lymphocytes population analysis in patients with chronic lymphocytic leukaemia B cells and autoimmune haemolytic anaemia (CLL-B + AIHA). We evaluated the following groups of patients: (1) with newly recognized CLL-B and co-existent AIHA (untreated), (2) after short-term treatment with corticosteroids, (3) after treatment with chemotherapy and corticosteroids. The control groups were made of patients with CLL-B without AIHA.

C-kit receptor (CD117) expression on plasma cells in monoclonal gammopathies.

The surface expression of CD117 antigen (c-kit) on plasma cells from 158 multiple myeloma (MM), 12 plasma cell leukemia (PCL), 7 MGUS, 7 IgM lymphoplasmacytic lymphoma patients and 10 healthy subjects has been analyzed by flow cytometry using triple staining with the monoclonal antibodies CD138, CD117 and CD38. The antigen expression intensity was calculated as relative fluorescence intensity (RFI) and for direct quantitative analysis the QuantiBRITE test (Becton Dickinson) was applied. Antibody bounding capacity (ABC) was calculated using QuantiCALC software.

c-Kit receptor (CD117) expression on myeloblasts and white blood cell counts in acute myeloid leukemia.

Background: The c-Kit receptor is considered to play a crucial role in hematopoiesis. Induction of mobilization of hematopoietic cells in the bone marrow requires cooperative signaling through c-Kit and c-Kit ligand pathway, and these interactions are important in the retention of stem cells within the bone marrow. Therefore, we analyzed c-Kit density on the leukemic myeloblasts of patients with acute myeloid leukemia (AML) in relation to white blood cell count (WBC) in the peripheral blood.

Hepatocellular peroxisome proliferation and DNA synthesis in Wistar rats treated with herbicide fluazifop.

The aim of this study was to determine the effect of herbicide fluazifop, on the early occurring changes in rat liver regarded as hepatic markers of peroxisome proliferators (PPs). Fluazifop was administered orally to male Wistar rats at increasing doses from 5.6 to 891 mg/kg body weight per day for 1, 2, 4, 7 and 14 consecutive days and peroxisome proliferation, induction of some peroxisome-associated enzymes and mitogenesis (S-phase, M-phase and percentage of binucleated hepatocytes) were studied.

Studies of early hepatocellular proliferation and peroxisomal proliferation in Wistar rats treated with herbicide diclofop.

A study was performed to determine whether diclofop (2-(4-(2,4-dichlorophenoxy) phenoxy)propionic acid), introduced as a herbicide, exhibits the properties of peroxisome proliferators (PPs). Diclofop was administered orally at 7-56 mg/kg body weight per day to male Wistar rats for 2, 4, 7 or 14 consecutive days and some effects regarded as early hepatic markers of PPs were studied. The early changes in rat liver, produced by short-term treatment with diclofop consisted of mitogenesis and, time- and dose-related increase in liver weight.

Early hepatic changes in rats induced by permethrin in comparison with DDT.

In this study permethrin [(3-phenoxyphenyl)-methyl-3-(2,2-dichloroethenyl)-2,2-dim ethylcyclopropanecarboxylate] and DDT [1,1-(2,2,2 trichloroethylidene)-bis-(4-chlorobenzene)] were compared in rats for their effects on early hepatic changes, proposed in the literature to be useful endpoints in screening for non-genotoxic hepatocarcinogenesis and/or liver tumour promotion. We compared the effects of both insecticides on the following endpoints: hepatomegaly, mitogenesis (DNA synthesis, mitotic activity, percentage of binuclear cells) and liver pathology. Male Wistar rats received permethrin (PERM) or DDT in one, three, five and 14 daily oral doses (at 24-h intervals) equivalent to 1/10 LD50.

Sensitivity of mouse lymphoid and nonlymphoid organs to Silesian air pollutants.

The toxic effect of Silesian air pollutants to mouse organs was examined. Histological changes were found in the examined lymphoid organs (thymus, spleen) as well nonlymphoid organs (liver, kidneys). The alterations in weight indexes of lymphoid organs were also observed.

[Organ cytotoxicity of selected polycyclic aromatic hydrocarbons in mice].

The cytotoxic effects of some PAHs: DMBA, 3-MC and B(a)P on the lymphatic organs, liver and kidney of mice have been investigated. These PAHs in doses of 100 mg/kg were dissolved in 0.5 ml 20% DMSO and were given intraperitoneally in female mice Balb/c.

Early hepatic changes induced in rats by two hepatocarcinogenic organohalogen pesticides: bromopropylate and DDT.

The aim of the present studies was to describe the effect of two organohalogen pesticides: DDT and bromopropylate, on early changes in rat liver, proposed in the literature to be useful endpoints in screening of non-genotoxic hepatocarcinogens and/or liver tumor promoters. We investigated the effects on the following endpoints: hepatomegaly, mitogenesis (DNA synthesis, mitotic activity, percentage of binuclear cells) and cytochrome CYP2B1-dependent monooxygenase induction. The histological and cytochemical changes in the liver were also recorded.

[The effect of selected polychlorinated hydrocarbon pesticides on proliferation of cells in rat liver (14 day experiment)].

The aim of present studies was to describe the effect of two organochlorine pesticides: nuarimol and DDT on the changes in rat liver, proposed in the literature to be useful endpoints in screening of non-genotoxic hepatocarcinogens and/or liver tumor promoters. The effects on the following endpoints: mitogenesis (DNA synthesis and mitotic activity), hepatomegaly as well as histological changes in rat liver have been investigated. Male Wistar rats received nuarimol or DDT in one, five and fourteen daily oral administration of the doses of 125 and 12 mg/kg b.

Early hepatic changes induced by nuarimol in rats.

It is commonly believed that in short-term tests hepatic cytochrome P-450b inducers stimulate liver enlargement and mitogenesis in the absence of overt hepatotoxic effects. In this investigation male Wistar rats received naurimol (an organochlorine pesticide) in one, three and five oral doses of 31.5, 62.

Protective action of vitamin E on the subcellular structures of lymphocytes intoxicated with pesticides in vitro.

Vitamin E was used as a protection for human lymphocytes in vitro against the toxic effects of dichlorvos, an organic phosphate pesticide. As the markers of the toxic changes in the lymphocytes lysosomes were chosen as a membrane structure, and nucleoli as a structure connected with protein synthesis. The obtained results were compared with the effects obtained previously in analogous studies after intoxication of lymphocytes with lindan and fenarimol.

Vitamin E protects human leucocytes against toxic effects of lindan in vitro.

Vitamin E (15 micrograms/ml and 60 micrograms/ml) was used for protection of human leucocytes against toxic effects of lindan (150 microM and 200 microM) in vitro. The protective effect of vitamin E manifested itself as maintenance of the phagocytic activity of granulocytes, the value of nucleologram in lymphocytes, and the proportion of euchrysin-negative lymphocytes near the values in controls.

[Changes in serum calcium level in rats as the effect of administration of fenarimol].

The Ca2+ concentration in rats serum after intragastrically fenarimol administration (doses 1/40, 1/20 and 1/10 LD50) during 5 days was investigated. The significant decrease of Ca2+ concentration in serum after 3 and 5 days of fenarimol administration was observed.

[Protection of human peripheral blood leukocytes with vitamin E and C from toxic effects of fenarimol in vitro].

The work was undertaken in a trial to protect leukocytes against the toxic effect of fenarimol with vitamin E and C. The experiment was carried out in vitro. These vitamins were chosen since they are the components of the physiological antioxidative mechanism in the cell.

Late toxic effects of long-term exposure to lindane in peripheral blood cells in rabbits. II. Erythrocyte changes.

After lindane administration in daily doses of 0.1 LD50 for one month (total dose 2.0 LD50) changes in the erythrocytes were found persisting for up to 4 months after withdrawal of the pesticide.

Late toxic effects of long-term exposure to lindane in peripheral blood cells in rabbits. I. Function impairment and structural disturbances in leucocytes.

Following lindane intoxication in daily doses of 0.1 LD50 during one month (total dose 2.0 LD50) functional changes were noted in granulocytes and structural changes in lymphocytes in peripheral blood of rabbits during 4 months after completion of lindane administration.

Degenerative changes, DNA synthesis and mitotic activity in rat liver following single and repeated exposure to fenarimol.

Degenerative changes were observed in liver of rats treated with single and repeated doses of fenarimol (250 mg/kg body weight x day). Regenerative changes i.e.