Rational engineering of ()-selective amine transaminase for the acceptance of bulky substrates.

Despite the plethora of information on ()-selective amine transaminases, the ()-selective ones are still not well-studied; only a few structures are known to date, and their substrate scope is limited, apart from a few stellar works in the field. Herein, the structure of ()-selective amine transaminase is elucidated to facilitate engineering towards variants active on bulkier substrates. The V37A variant exhibited increased activity towards 1-phenylpropylamine and to activity against 1-butylamine.