Expression of MicroRNA-200a/b/c in the Mediobasal Hypothalamic Nuclei with Aging.

Background: MicroRNAs (miRNAs) belong to small non-coding RNAs that coordinate the expression of cellular genes at the post-transcriptional level. The hypothalamus is a key regulator of homeostasis, biological rhythms and adaptation to different environmental factors. It also participates in the aging regulation.

Ontogenetic Changes in the Expression of the Lin28 Protein in the Rat Hypothalamic Tuberal Nuclei.

The hypothalamus is a primary regulator of homeostasis, biological rhythms and adaptation to different environment factors. It also participates in the aging regulation. The expression of neurons containing Lin28 was studied by immunohistochemistry in male rats aged 2, 6, 12, and 24 months in the tuberal region of the rat hypothalamus.

Changes of discharge properties of neurons from dorsomedial hypothalamic nuclei during aging in rats.

The hypothalamus is a vital brain center that is participated in the integration of the endocrine and nervous systems and control of the homeostasis and aging. Spontaneous firing activity from single neurons of the dorsomedial hypothalamic nucleus (DMN) was studied extracellularly in vivo in urethane-anaesthetized rats. The discharge patterns of the majority of DMN neurons were irregular, including periods of relatively stable activity interrupted by pauses.

Expression of calbindin and calretinin in the dorsomedial and ventromedial hypothalamic nuclei during aging.

The hypothalamus is involved in the regulation of rhythms, autonomic, endocrine, and behavioral functions and may also participate in aging development and control. The aim of this work was to study the expression of calbindin (CB) and calretinin (CR) in the ventromedial (VMH) and dorsomedial (DMH) hypothalamic nuclei in young and old rats of both sexes by immunohistochemistry and western blotting. In young animals, the largest number of CB-immunoreactive (IR) neurons was detected in the ventral part of DMH (DMHv) and smaller percentage was found in its dorsal part (DMHd), in the dorsomedial part of the VMH (VMHdm) and in the ventrolateral part of the VMH (VMHvl).

Changes of nNOS expression in the tuberal hypothalamic nuclei during ageing.

The hypothalamus is the most important integrator of autonomic and endocrine regulation in the body and it also has a fundamental role in ageing development and lifespan control. In order to better understand the role of NO-ergic system in the hypothalamic regulation of ageing, the purpose of this study was to investigate the expression of neuronal nitric oxide synthase (nNOS) in the arcuate (ARC), ventromedial (VMH) and dorsomedial (DMH) hypothalamic nuclei in young (2-3-month-old) and old (24-month-old) male and female rats using immunohistochemistry and western blot analysis. In young animals, only single nNOS-immunoreactive (IR) neurons were detected in ARC, and nNOS-IR neurons were found in the VMH (19 ± 3.