Potentially Bio-Accessible Metabolites from an Extract of Fruit after Gastrointestinal Digestion In Vitro and Gut Microbiota Ex Vivo Treatment.

Targeting pancreatic lipase and α-amylase by digestion-derived fractions of ethanolic-aqueous (60%, /) extract from fruit (CM) in relation to the control and prevention of metabolic disorders, including diabetes, was the first purpose of the present study. Taking into consideration the significance of bio-accessibility of compounds, we attempted to identify metabolites of CM after gastrointestinal digestion in vitro, as well as their kinetic changes upon gut microbiota treatment. The digestion of extract was simulated with digestive enzymes in vitro and human gut microbiota ex vivo (1 h, 3 h, 6 h, 24 h), followed by chromatographic analysis using the UHPLC-DAD-MS method.

and -A Comparison of Antioxidant and Immunomodulatory Activities of Standardized Fruit Extracts in Human Neutrophils and Caco-2 Models.

Fruits of and are representative plant materials traditionally used in Europe and Asia, respectively, in the treatment of diabetes and diabetes-related complications, which are often mediated by pathogenic inflammatory agents. Additionally, due to the fact of mutual infiltration of Asian and European medicines, the differentiation as well as standardization of traditional prescriptions seem to be crucial for ensuring the quality of traditional products. The objective of this study was a comparison of biological activity of extracts from fruits of and by an assessment of their effect on reactive oxygen species (ROS) generation in human neutrophils as well as cytokines secretion both in neutrophils (tumor necrosis factor , TNF- ; interleukin 8, IL-8; interleukin 1, IL-1) and in human colon adenocarcinoma cell line Caco-2 (IL-8).

Inhibition of Digestive Enzymes and Antioxidant Activity of Extracts from Fruits of , subsp. and -A Comparative Study.

The fruits of some species (dogwoods) are used in traditional medicine and considered potential anti-diabetic and hypolipemic agents. The aim of the study was to determine the ability of extracts from (CA), (CF), and (CS) to inhibit digestive enzymes namely -amylase, pancreatic lipase, and -glucosidase, as well as isolation of compounds from plant material with the strongest effect. In addition, the phytochemical profile and antioxidant activity of extracts from three dogwoods were compared with HPLC-DAD-MS/MS and DPPH scavenging assay, respectively.