[Use of allogeneic mesenchymal stem cells in the treatment of intestinal inflammatory diseases].

Aim: to determine the whether mesenchymal stem cells (MSC) may be used in the treatment of patients with chrOnic intestinal inflammatory diseases (IID).

Subjects And Methods: Thirty-nine patients with ulcerative colitis (UC) (Group 1) and 11 with Crohn's disease (CD) (Group 2) were examined. Comparative groups included 30 patients with UC (Group 2) and 10 with CD (Group 4).

[Allogeneic mesenchymal stromal cells in patients with ulcerative colitis: two years of observation].

Unlabelled: The aim of the study was to determine the efficacy and safety of mesenchymal stromal cells (MSCs) of bone marrow in the treatment of patients with ulcerative colitis (UC).

Materials And Methods: The study included 44 patients with ulcerative colitis (UC), which was implemented MSC transplantation, 40 patients with UC who received standard therapy with mesalazane (salofalka) 4-6 g/day and corticosteroids (prednisone)--1-2 mg/kg, azathioprine--1.5 mg/kg methotrexate 20-50 mg/m2, and 12 patients who underwent induction and maintenance of infliximab therapy.

[Application of allogeneic mesenchymal stem cells in complex patients treatment with ulcerative colitis].

Systemic transplantation of mesenchymal stem cells (MSC) is known to promote reparative process in a number of tissue damage as well as mucous healing one. It provided the basis for our study which was aimed to the effect of systemic transplantation of allogenic MSC (intravenous transfusion) in complex therapy of patients with Ulcerative Colitis.

["Ischemia/reperfusion" for stem cells of two "critical" cell renewal systems of organism].

In this article is presented the result of the experiments on mice-hybrids F1(CBA x C57B1/6), which indicates the presence of the reaction of "ischemia/reperfusion" for stem cells of two "critical" cell renewal systems of organism (bone marrow and intestinal epithelium) during the irradiation under the conditions of hypoxic radioprotector application. The additional injection of the source of nitric oxide radicals-sodum nitroprusside (SNT) to the mice right after the irradiation under the conditions of hypoxic protection by serotonin, resulted the substantial increase of the survival rate of hematopoietic stem cells (registered by the methods of endogenous and exogenous colony forming in spleen) and stem cells of intestinal epithelium (registered by the method of intestinal "microcolonies"). The similar radioprotective effect was also registered during the test of survival rate of mice under tests of "bone marrow" and "intestinal" forms of radiation lethality that is evidence of the importance of the realization of phenomenon "ischemia/reperfusion" in the reaction of whole organism on the acute radiation injury.

[Radioprotective and antistressful properties of nitric oxide production modulators].

The radioprotective and antistressful activities of L-arginine and the "Pronumol" preparation, in which L-arginine is contained in the complex of proteins with nucleic acids, were studied. In mice repeated peroral intake of L-arginine and "Pronumol" partially prevented radiation-induced and stress-induced lipid peroxidation and DNA degradation in thymus, increased hemopoietic stem cell survival, and prevented an increase in chromosome aberration frequency in bone marrow cells of irradiated mice. When repeatedly administered per os before irradiation, "Pronumol" increased survival of intestinal stem cells in irradiated mice and prevented thymus cell devastation induced by radiation and stress.

[Radioprotective effect of E. coli endotoxin on hematopoietic stem cells is partially suppressed by inhibiting production of nitric oxide by administering N-omega-nitro-L-arginine].

Lipopolysaccharide of E. coli (LPS) injected to mice one day before the total gamma-irradiation caused a substantial increase in the level of endogeneous colonies formed in the spleen. It is known that this type of endotoxin may result in considerable production of nitric oxide from macrophages in different tissues.

[A modification in the radiation reaction of normal and tumor tissues in mice induced by N-methylformamide].

The effect of potential differentiation-inducing agent N-methylformamide on radiation response of murine normal and tumor cells (Lewis lung carcinoma, hematopoietic tissue and jejunum epithelial stem cells) was studied. The agent reduced or not altered radiation damage of tumor and epithelial cells in mice receiving NMF before irradiation. Sensitization to radiation was observed in endogenous spleen colony forming hemopoietic stem cells.

[Radiobiological aspects of increased radioresistance of murine epithelial stem cells from patches of Peyer].

The fraction of the survived intestinal crypts associated with patches of Peyer is higher to those non-patch-associated crypts of mice for three inbred strains. The difference in the survival between associated and non-patch-associated crypts increases with dose of gamma-irradiation. This difference for old mice is less than for young mice.

[Radiosensitizing effect of hyperthermia (41.5C, 30 min) in stem cells of mouse spermatogenic epithelium].

Mouse testes preheated up to 41.5 degrees C for 30 min exhibit an increased radiosensitivity of testis epithelium stem cells. High death rate is described by a shift (by approximately 4 Gy) in the "dose-response" curve to the Abscissa left.

[The effect of gas hypoxia (10% O2) and dextran sulfate on the radiosensitivity of the stem cells of the intestinal epithelium in mice].

Injection of dextran sulphate before irradiation was shown to protect jejunal epithelium stem cells (D0 increased from 1.13 to 1.82 Gy).

[The radioprotective effect of a hypoxic respiratory mixture (8% O2) for intestinal epithelial stem cells during single and fractionated irradiation of mice].

The method of intestinal "microcolonies" was used to study the radioprotective effect of a gas mixture, containing 8% of O2, on mice subjected to single and fractionated (5 fractions for 30 min) irradiation. The protective effect was indicated by a decreased slope of dose curves of the stem cell injury; the extrapolation number decreased simultaneously. So the values of dose modifying factors (DMF) were higher, when calculated by D0 ratio (where they amounted to 1.

[Radiobiology of stem cells in intestinal epithelium. Effect of single and multiple preliminary sublethal irradiation of mice on the dose dependence of the survival of stem cells in small intestine epithelium].

After a single or three-fold whole body irradiation of mice with a dose of 4 Gy and the time interval for the proliferation to be restored (5 days or 3 weeks) the survival curve for stem cells of small intestine epithelium with regard to radiation dose was the same as that for non-preirradiated mice. This indicated that the proliferative potential of stem cells in these experimental conditions was not reduced.

[Radiosensitivity of stem cells in the spermatogenic epithelium of mice of different strains and different ages].

In experiments on CBA and BALB/c male mice (3 months of age) and F1(CBA X C57BL/6) hybrids (at the age of 3, 12, and 24 months) a difference was noted in the radiosensitivity of spermatogenic epithelium stem cells displayed by the changes in their colony-forming ability in testicular tubules 42 days following local 60Co-gamma-irradiation. The older the hybrid mice the smaller was the number of spermatogenic epithelium stem cells.

[Radiosensitizing and damaging action of hyperthermia on different biological systems. Radiosensitizing and damaging action of hyperthermia on mouse hematopoietic stem cells].

Dose dependences of heat-induced inactivation of CFUs have been described for the temperature range of 41 to 44 degrees C. The energy of activation of the processes of thermal death was 100-120 kcal/M. CFUs were more resistant to heating than the cells of mouse leukaemia.

[Age-related changes in the radiosensitivity of animals and critical cell systems. Survival of stem cells of the small intestine epithelium 4-5 days after death in mice of various ages].

Mice of three strains exhibited similar changes in radiosensitivity tested with a reference to "intestinal" death: juvenile and old animals were more radiosensitive. No age-related changes were detected in radiosensitivity of stem cells of the small intestine epithelium estimated with a reference to average lethal dose D0.