[Effect of hardware balance trainings on postural control, risk of falls and cognitive function on elderly people with chronic cerebral ischemia. (Randomized controlled trial)].

Introduction: As a prevention of falls in elderly people with chronic cerebral ischemia, it is necessary to carry out rehabilitation measures with the inclusion of training aimed at improving statolocomotor and cognitive functions, improving proprioceptive sensitivity and reaction speed to external stimuli.

Aims: To compare the effectiveness of inclusion of hardware balance training on Huber («LPG-Systems», France) and C-mill («Physiomed Elektromedizin AG», Germany) simulators in complex postural control rehabilitation programs for elderly patients with chronic cerebral ischemia (CCI).

Material And Methods: The study included 48 patients (19 men, 29 women), 81% of whom had moderate cognitive impairment.

Paramagnetic Defects and Thermoluminescence in Irradiated Nanostructured Monoclinic Zirconium Dioxide.

The ESR spectra of nanostructured samples of monoclinic ZrO irradiated by electrons with energies of 130 keV, 10 MeV, and by a beam of Xe ions (220 MeV) have been studied. It has been established that irradiation of samples with electrons (10 MeV) and ions leads to the formation of radiation-induced centers in them. Thermal destruction of these centers is observed in the temperature range of 375-550 K for electron-irradiated and 500-700 K for ion-irradiated samples.

[Effectiveness of physical therapy after total hip arthroplasty at the third stage of rehabilitation using a telemedicine program].

Objective: To assess the quality of life and efficacy of telemedicine technologies for rehabilitation after total hip replacement.

Material And Methods: The study included 60 patients aged 40-75 years after total hip replacement. All patients were divided into two groups by simple randomization.

[The role of systemic ozone therapy in the rehabilitation of patients after COVID-19].

Objective: To evaluate the effectiveness and safety of ozone therapy in rehabilitation of patients with previous COVID-19.

Material And Methods: A randomized controlled clinical trial included 51 patients aged 29 - 78 years with SARS-CoV-2 pneumonia (J12.8).

[Efficiency of balance training with stabilizing platform in early postoperative rehabilitation of patients after arthroplasty].

Objective: To evaluate the effectiveness of balance training on stabilizing platform (HUBER360) in early postoperative rehabilitation of patients after total knee arthroplasty.

Material And Methods: We examined 60 patients after total knee arthroplasty. Mean age of patients was 69.

[Recovery of social and everyday skills after a complex of functional spatially oriented rehabilitation in elderly patients with cerebral stroke].

Objective: To analyze the effectiveness of social recovery (trip to the store by bus and making purchases) in elderly patients with stroke in the right middle cerebral artery undergoing a course of specialized trainings in a complex of functional spatially oriented rehabilitation.

Material And Methods: The study involved 44 patients with previous stroke (mean age 70.8±3.

Multifunctional Elastic Nanocomposites with Extremely Low Concentrations of Single-Walled Carbon Nanotubes.

Stretchable and flexible electronics has attracted broad attention over the last years. Nanocomposites based on elastomers and carbon nanotubes are a promising material for soft electronic applications. Despite the fact that single-walled carbon nanotube (SWCNT) based nanocomposites often demonstrate superior properties, the vast majority of the studies were devoted to those based on multiwalled carbon nanotubes (MWCNTs) mainly because of their higher availability and easier processing procedures.

Flexible Polycaprolactone and Polycaprolactone/Graphene Scaffolds for Tissue Engineering.

Developing bone scaffolds can greatly improve the patient's quality of life by accelerating the rehabilitation process. In this paper, we studied the process of composite polycaprolactone supercritical foaming for tissue engineering. The influence of graphene oxide and reduced graphene oxide on the foaming parameters was studied.

Bridging the gaps in 3D structure of the inositol 1,4,5-trisphosphate receptor-binding core.

Calcium concentration is strictly regulated in all cells. The inositol 1,4,5-trisphosphate receptor (IP(3)R), which forms a homotetrameric Ca2+ release channel in the endoplasmic reticulum, is one of the key molecules responsible for this regulation. The opening of this channel requires binding of two intracellular messengers, which are inositol 1,4,5-trisphosphate (IP(3)) and Ca2+.

[Lanthanides induce neurotransmitter release from the vesicular pool in rat brain synaptosomes].

The influence of lanthanoids on exocytosis was investigated. It was shown that gadolinium increases the spontaneous release of the glutamate nonmetabolizing analogue [3H]D-aspartate. It was established using the fluorescent dye acridine orange that gadolinium and lanthanum induce exocytosis.

Role of calcium in exocytosis induced by hypotonic swelling.

We studied the influence of Ca(2+) on exocytosis induced by hypotonic shock using the fluorescent dyes acridine orange FM1-43 and FM2-10. It was shown using acridine orange that lowering osmolarity to 230 mOsm/L induces exocytosis both in calcium-containing and calcium-free media. By contrast, we were able to demonstrate calcium dependence of exocytosis using styryl dyes.

[The dynamics of the domains of the IP3-binding site of the inositol-1,4,5-triphosphate-sensitive calcium channel, induced by inositol-1,4,5-triphosphate and calcium].

The dynamics of the inositol-1,4,5-triphosphate-sensitive calcium channel after binding of inositol-1,4,5-triphosphate and Ca2+ was analyzed by the Monte Carlo minimization technique. It was shown that the binding of Ca2+ with the unliganded receptor (channel) leads to a turning of the beta-sheet domain relative to the alpha-helical domain with the formation of the receptor conformation that is open for the entry of ions into the cytoplasmic channel vestibule, sterically closed for their passage through the vestibule in the part adjacent to the alpha-helical domains, and unfavourable for subsequent binding of inositol-1,4,5-triphosphate with the receptor. When both co-agonists bind to the receptor, the structure rearrangements induced eliminate both these steric obstacles for the passage of ions through the IP3-binding domain: one at the entrance of the channel cytoplasmic vestibule and the other that is placed deeper in the vestibule near the alpha-domains.

Calcium regulates the mode of exocytosis induced by hypotonic shock in isolated neuronal presynaptic endings.

A decrease in the osmolarity of incubation medium is accompanied by calcium influx in neuronal presynaptic endings. We studied the influence of Ca2+ on exocytosis induced by hypotonic shock using the hydrophilic fluorescent dye acridine orange and the hydrophobic fluorescent dye FM2-10. It was shown using acridine orange that lowering of osmolarity to 230 mOsm/l induces exocytosis both in calcium-containing and calcium-free medium.

Influence of hypotonic shock on glutamate and GABA uptake in rat brain synaptosomes.

Hyponatremia leads to hyperexcitability of neurons, seizures, and coma. It is well established that uptake of neurotransmitters is a sodium-dependent process. Therefore, we suggest that inhibition of neurotransmitter uptake can lead to the clinical manifestations of hyponatremia.

[Influence of calcium ionophore A23187 on neurotransmitter release in rat brain synaptosomes].

The effect of calcium ionophore A23187 on the release of nonmetabolizable glutamate analogues [3H]D-aspartate and the exocytosis registered by fluorescent dyes in synaptosomes was investigated. It was shown that A23187 is able to induce neurotransmitter release both in calcium-containing and calcium-free medium, the effect in the latter case being more pronounced. Calcium ionophore is able to induce exocytosis registered by acridine orange and FM 2-10.

[Determination of tyrosine- and tryptophan-containing peptides in plasma by absorption in UV spectral region].

A method for determining the cumulative concentration of tyrosine- and tryptophan-containing peptides (TSP and TPP) in blood plasma: 0.2 ml of blood plasma is added 0.1 ml of 1.

[Effect of osmolar concentration of culture media on exocytosis in isolated presynaptic nerve endings of rat brain ].

The effect of hypotonic and hypertonic shock on exocytosis in rat brain synaptosomes was studied using the fluorescent dye acridine orange. It was shown that an increase in medium osmolarity leads to calcium-independent exocytosis. The response of the probe was directly proportional to the amount of osmolithes added.

Effect of low pH on glutamate uptake and release in isolated presynaptic endings from rat brain.

The effect of acidification of the incubation medium on the membrane potential and glutamate uptake and release was studied in isolated presynaptic neuronal endings (synaptosomes) from rat brain. Using the fluorescent probe diS-C3-(5), a rapid depolarization of plasma membrane was detected at pH 6.0, most probably as a result of the inhibition of the sodium pump and potassium channel blockade.

Characteristics of the hypoosmosis-induced calcium response in isolated nerve terminals of rat brain.

Background: Cerebral edema contributes significantly to morbidity and death in many common neurological disorders. Ca2+ ions play a important role in the development of pathophysiological reactions in nerve cells. The aim of our research was to elucidate the mechanism of the osmotically-induced entrance of Ca2+ into nerve terminals (synaptosomes) and to estimate the involvement of endoplasmic reticulum Ca2+ stores in intrasynaptosomal Ca2+-dependent processes in hypoosmotic swelling.

Fluorescent probing of the ligand-binding ability of blood plasma in the acute-phase response.

The acute-phase response alters the composition of carrier proteins in plasma, which may affect the blood deposition and transport of biomediators and drugs. The effect of the acute-phase response on the ligand binding ability of plasma was studied in leukemic children with and without systemic inflammation (sepsis and septic shock). To target different transport proteins, differentially charged fluorescent dyes were used: anionic ANS (8-anilinonaphthalene-1-sulfonate), uncharged Nile red, and cationic Quinaldine red.

Effects of nitric oxide donors on Ca2+-dependent [14C]GABA release from brain synaptosomes: the role of SH-groups.

Nitric oxide (NO) modulates processes of synaptic transmission at pre- and postsynaptic levels. In the present work we studied the mechanisms of action of NO on [gamma-14C]amino-n-butyric acid ([14C]GABA) release in rat cortical synaptosomes. NO donors--S-nitroso-L-cysteine and hydroxylamine (but not sodium nitroprusside)--inhibited the neurotransmitter efflux in a concentration range from 10 microM to 1 mM.

[Assessment of body intoxication as a balance between accumulation and binding of toxin in plasma].

A novel approach to assessing endogenic intoxication (EI) as an imbalance between toxin accumulation and binding by albumin in blood plasma is proposed. The intoxication criterion (IC) is determined by the ratio of the content of medium-weight molecules (MWM) and effective albumin concentration (EAC): IC = MWN/EAC. In children with oncohematological diseases the development of EI is associated with a 4-fold drop of MWM content and a twofold decrease of EAC, and hence, the imbalance between these two values increases 9-10 times.

Bioenergetic response of isolated nerve terminals of rat brain to osmotic swelling.

The swelling of nerve terminals of rat brain in a hypotonic medium (230 mOsm) induced the potential-independent entrance of 45Ca2+ into synaptosomes and intrasynaptosomal mitochondria that changed the energy status of synaptosomes, the rate of O2 consumption and the content of ATP being decreased. The ratio ATP/ADP decreased from 6.5 +/- 0.

Different modes of ozone-induced lipid oxidation in Candida utilis yeast cells and isolated membrane preparations.

Significant differences in the development of ozonolysis of lipids in membrane preparations and intact cells of the Candida utilis yeast were revealed. First, unlike isolated membranes, in which lipid modifications can be initiated by low ozone doses (< 0.5 micromol O3/mg protein) and develop proportionally to the treatment dose, in intact yeast cells, even the most ozone-sensitive sterols and nitrogen-containing phospholipids (phosphatidylcholine and phosphatidylethanolamine) did not undergo oxidative destruction at doses up to 6.