Preserved tissue allografts in reconstructive surgery.

Results of treatment with three various kinds of allografts: lyophilized bone, deep frozen bone and cartilage preserved in physiological solution, all of them radiation-sterilized are presented. We believe that this presentation may be helpful in estimating the tissue bank's allografts and in establishing indications and contraindications in the application of allografts in surgery.The 'indices of coincidence' were compared in a group of 1014 patients after bone (lyophilized and radiation-sterilized) transplantation.

Allogenic preserved costal cartilage in reconstructive surgery.

Allogenic, preserved cartilage is often used for reconstruction of face. This study was undertaken to analyze the effects of cartilage transplantation. In analyzed group of 437 patients after cartilage transplantation, 42.

Bone banking for transplantation in orthopaedic reconstructions.

Results of orthopaedic reconstructions in 1014 patients with the use of preserved allogenic bone grafts are presented. The method of grafts preservation seems to be of significance in surgical applications; deep-freezing is more favorable in more comparison with lyophilization. The result of treatment after bone transplantation is dependent on numerous biological and clinical variables.

Therapeutic effects of transplantation of lyophilized and radiation-sterilized, allogeneic bone.

Lyophilized radiation-sterilized bone graft operations were analyzed according to 27 variables in 1014 patients with two-year follow-up results. In 91.3% of patients, the result of treatment was "very good" (full-health restoration) or "satisfactory" (restoration with some small disorders).

Biological properties of preserved human dura mater grafts from different-age donors, tested in xenogenic system of transplantation.

Selected biological properties of human mater grafts obtained from different-age donors and preserved with two methods were tested in a xenogenic system of transplantation. The Regional Lymph Node Blastic Cell Reaction Test revealed relatively low immunogenicity of the material. Microscopic analysis showed that trypsinized grafts are rebuilt at a slower rate and evoke less local reaction than the ethanol-stabilized grafts.

Some biological properties of preserved bovine fascia enriched with pulverized calf cartilage.

The immunogenicity of trypsinized and radiation-sterilized bovine fascia enriched with pulverized calf cartilage was tested by the regional lymph node blastic cell reaction. The influence of these grafts on the adjacent tissue and on regeneration processes was examined histologically. After transplantation into mice the grafts stimulated growth of new connective tissue and did not evoke an increase of the blastic cell reaction in the regional lymph nodes.

Some biological properties of bovine trypsinized fascia xenografts.

Trypsinized, lyophilized and radiation-sterilized bovine fascia grafts were used for treatment of muscle and peritoneal defects induced in mice. The survival rate, general and surgery region condition of normal and pregnant mice after transplantation were investigated. The influence of grafts on the adjacent tissues and on regeneration processes was also examined.

Investigations on immunogenicity of bovine fibrous connective tissue.

The immunogenicity of fibrous connective bovine tissue preserved by different methods was tested by a regional lymph node blastic cell reaction. The results were presented after transplantation of 10 experimental groups of grafts.

Immunogenicity of fresh and preserved cortical and cancellous allogeneic bone grafts as tested by modified migration inhibition test in mice.

Mice were grafted with allogeneic compact and cancellous bone. The bone used was fresh and preserved by either lyophilization or deep freezing. Preserved grafts were radiosterilized by a dose of 3.