SIRT1 and HSP90α feed-forward circuit safeguards chromosome segregation integrity in diffuse large B cell lymphomas.

Diffuse large B-cell lymphoma (DLBCL) is the most common aggressive non-Hodgkin lymphoma in adults, exhibiting highly heterogenous clinical behavior and complex molecular background. In addition to the genetic complexity, different DLBCL subsets exhibit phenotypic features independent of the genetic background. For example, a subset of DLBCLs is distinguished by increased oxidative phosphorylation and unique transcriptional features, including overexpression of certain mitochondrial genes and a molecular chaperone, heat shock protein HSP90α (termed "OxPhos" DLBCLs).

Can Assessment of Rheological Properties of Whole Blood and Plasma Be Useful in the Diagnosis of Tinnitus? A Pilot Study.

Tinnitus is a sensation of ringing in the ears in the absence of any physical source in the environment. Between 9-35% of adults experience some form of tinnitus. Common causes of tinnitus include noise, head injury, ototoxic substances, as well as disorders of blood and blood vessels.

Roughness of the Surface of Zirconia Reinforced Lithium Disilicate Ceramic Treated by Different Procedures.

Lithium disilicate and zirconia are the two most popular materials for aesthetic and dental prosthetic work; however, due to their limitations, a new material is being researched, namely zirconia-reinforced lithium disilicate, the surface of which is treated with different procedures to achieve the best possible surface properties. In this study, the surface of zirconia-reinforced lithium disilicate glass-ceramic was treated using different methods (conventional and laser) to determine the effects of the treatment procedures on the surface properties and surface roughness to achieve a higher strength of adhesion from the self-adhesive resin cement to zirconia-reinforced lithium disilicate. The treated surfaces were investigated using profilometry, X-ray diffraction and energy dispersive X-ray fluorescence.

Body Balance Analysis in the Visually Impaired Individuals Aged 18-24 Years.

Background: Proper body balance is maintained by both sensory, i.e., vision, hearing, vestibular, and proprioception, and motor nervous systems.

Self-Massage and Low-Level Laser in Treatment of Masseter Myalgia: Short-Term Effect.

Introduction: The aim of this study was to explore the efficiency of massage in the treatment of masseter myalgia compared with biostimulatory laser.

Materials And Methods: The instrument Diagnostic Criteria for Temporomandibular Disorders was used to select subjects and monitor treatment outcome. 54 subjects with masseter myalgia of more than 3 months in duration were invited to participated and 42 cases were available at the end and analyzed.

Influence of Different Surface Pretreatments of Zirconium Dioxide Reinforced Lithium Disilicate Ceramics on the Shear Bond Strength of Self-Adhesive Resin Cement.

Aim: To analyze the influence of different surface pretreatments of zirconium dioxide reinforced lithium disilicate ceramics on the shear bond strength of self-adhesive resin cement.

Materials And Methods: Eighty-four zirconium reinforced lithium disilicate disc Vita suprinity (Vita Zahnfabrick, Bad Säckingen, Germany) 14x12x2 mm specimens were fabricated according to the manufacturer's recommendations. The specimens were embedded in acrylic resin blocks and randomly divided in seven groups (n=12/each) accorrding to the treatment: Group 1- 10% hydrofluoric acid; Group 2- silane; Group 3- hydrofluoric and silane; Group 4- sandblasting with silane; Group 5- Er: YAG laser+ silane; Group 6- Nd: YAG laser + silane; and the control group, in which the specimens were not treated.

Inhibition of PIM Kinases in DLBCL Targets MYC Transcriptional Program and Augments the Efficacy of Anti-CD20 Antibodies.

The family of PIM serine/threonine kinases includes three highly conserved oncogenes, and , which regulate multiple prosurvival pathways and cooperate with other oncogenes such as . Recent genomic CRISPR-Cas9 screens further highlighted oncogenic functions of PIMs in diffuse large B-cell lymphoma (DLBCL) cells, justifying the development of small-molecule PIM inhibitors and therapeutic targeting of PIM kinases in lymphomas. However, detailed consequences of PIM inhibition in DLBCL remain undefined.

A comparison of the international protocols for the forensic assessment of conflict-related sexual violence victims.

International protocols for forensic investigations are often created by committee and in isolation. When field tested, the results of such tests are rarely reported to the wider forensic community. This study presents a comparative study of one such protocol, the International Protocol on the Documentation and Investigation of Sexual Violence in Conflict: Best Practice in the Documentation of Sexual Violence as a Crime or Violation of International Law.

Rebelled epigenome: histone H3S10 phosphorylation and H3S10 kinases in cancer biology and therapy.

Background: With the discovery that more than half of human cancers harbor mutations in chromatin proteins, deregulation of epigenetic mechanisms has been recognized a hallmark of malignant transformation. Post-translational modifications (PTMs) of histone proteins, as main components of epigenetic regulatory machinery, are also broadly accepted as therapeutic target. Current "epigenetic" therapies target predominantly writers, erasers and readers of histone acetylation and (to a lesser extent) methylation, leaving other types of PTMs largely unexplored.

Addressing the Lack of Forensic Scientific Evidence and Expertise in the "Trigger Mechanisms" of Genocide Investigations.

The impetus to begin a legal investigation or prosecution of the crime of genocide is a "trigger mechanism," which serves as the prima facie case against the accused state or actor. Unlike domestic cases of homicide, the trigger mechanisms for international genocide investigations to date have not included any forensic evidence nor have they sought input from forensic scientists. The jurists tasked with addressing the trigger mechanisms were fully capable of assessing forensic evidence but unable to generate it.

Audiological evaluation of patients with cleidocranial dysplasia (CCD).

Objective: The description of clinical manifestation of hearing problems in cleidocranial dysplasia (CCD) remains limited and incomplete, since CCD constitutes a rare congenital disorder. The study aims to provide a complex panel analysis of the auditory system in patients suffering from the disease.

Patients And Methods: The study group consisted of 4 children with CCD (aged: 12-15), who underwent orthodontic treatment.

Healthcare Utilization and Patients' Perspectives After Receiving a Positive Genetic Test for Familial Hypercholesterolemia.

Background: The MyCode Community Health Initiative (MyCode) is returning actionable results from whole exome sequencing. Familial hypercholesterolemia (FH) is an inherited condition characterized by premature cardiovascular disease.

Methods: We used multiple methods to assess care in 28 MyCode participants who received FH results.

The Use of the Chromatin Immunoprecipitation Technique for In Vivo Identification of Plant Protein-DNA Interactions.

Two-hybrid systems allow for the identification of proteins that physically interact in the context of biological processes. In the cases where these proteins interact with DNA it is essential to define their binding properties with specific regions of the genome to shed light on the intricate gene regulatory networks that modulate the biological response of interest. The chromatin immunoprecipitation (ChIP) protocol described here provides a powerful means to identify the DNA-binding sites of transcription factors, proteins involved in chromatin remodeling processes, or histone marks that modulate gene expression in eukaryotes and specifically in plants like the model species Arabidopsis thaliana.

Highly Charged Rydberg Ions from the Coulomb Explosion of Clusters.

Ion emission from a nanoplasma produced in the interaction of intense optical laser pulses with argon clusters is studied resolving simultaneously charge states and recoil energies. By applying appropriate static electric fields we observe that a significant fraction of the ions Ar^{q+} (q=1-7) has electrons with binding energies lower than 150 meV; i.e.

High performance charge-state resolving ion energy analyzer optimized for intense laser studies on low-density cluster targets.

We report on a versatile ion analyzer which is capable to resolve ion charge states and energies with a resolution of E/ΔE = 100 at 75 keV/nucleon. Charge states are identified by their characteristic deflection in a magnetic field, whereas the ion energies are independently determined by a time-of-flight measurement. To monitor the signals a delay-line detector is used which records ion impact positions and times in each laser shot.

β-carbonic anhydrases and carbonic ions uptake positively influence Arabidopsis photosynthesis, oxidative stress tolerance and growth in light dependent manner.

Carbonic anhydrases (CAs) catalyse reversible interconversion of CO and water into bicarbonate and protons and regulate concentration of CO around photosynthetic enzymes. In higher plants the CAs are divided into three distinct classes α, β and γ, with members off each of them being involved in CO uptake, fixation or recycling. The most abundant group is βCAs.

Chromatin and epigenetics in all their states: Meeting report of the first conference on Epigenetic and Chromatin Regulation of Plant Traits - January 14 - 15, 2016 - Strasbourg, France.

In January 2016, the first Epigenetic and Chromatin Regulation of Plant Traits conference was held in Strasbourg, France. An all-star lineup of speakers, a packed audience of 130 participants from over 20 countries, and a friendly scientific atmosphere contributed to make this conference a meeting to remember. In this article we summarize some of the new insights into chromatin, epigenetics, and epigenomics research and highlight nascent ideas and emerging concepts in this exciting area of research.

Surgical, functional and audiological evaluation of new Baha(®) Attract system implantations.

Bone-anchored hearing aids are well-established solutions for treatment of hearing-impaired patients. However, classical systems with percutaneous abutments have disadvantages concerning aesthetics, hygiene and adverse soft tissue reactions. The study aimed to evaluate surgical, functional and audiological results of a new Baha(®) Attract system, in which the sound processor is attached by magnetic force.

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo.

Intricate gene regulatory networks orchestrate biological processes and developmental transitions in plants. Selective transcriptional activation and silencing of genes mediate the response of plants to environmental signals and developmental cues. Therefore, insights into the mechanisms that control plant gene expression are essential to gain a deep understanding of how biological processes are regulated in plants.

Gabapentin-induced changes of plasma cortisol level and immune status in hysterectomized women.

Aim: We have examined the effects of gabapentin (GBP) on stress-related changes of cortisol and catecholamines in patients who underwent hysterectomy because of uterine fibrinoids. Additionally, we have observed the effect of GBP on the immune status in the acute stress response to surgery.

Methods: Sixty patients scheduled for an abdominal hysterectomy were randomly assigned to the GBP administration 1h before surgery (n=30 pts), or to the placebo group (n=30 pts).

Prosthetic modalities used to treat cleft palate patients in a university clinic: a 10-year review.

Cleft palate patients are not usually seen in general dental clinics, but this congenital anomaly is one of the most frequent of cases. General dental practitioners are usually unwilling or/and not sufficiently trained to treat such patients for whom rehabilitation and interdisciplinary cooperation is often needed. The aim of this study was to determine the incidence of prosthetic modalities most frequently used by licensed prosthodontists for prosthetic rehabilitation of cleft palate patients.

Denture relining as an indicator of residual ridge resorption.

Aim: Residual ridges change in shape and reduce in size due to alveolar bone resorption. Therefore, the purpose was to investigate changes in denture base thickness (DBT) and interocclusal rest space (IRS) with regard to the denture relining procedure.

Methods: The IRS and DBT were measured (before and after denture relining) in the case of 74 patients and their removable dental prostheses by using a precise sliding gauge.

Patient's satisfaction with removable dentures after relining.

Aim: To reveal patients' satisfaction with their old, usually poorly fitting dentures and the effect of denture relining to the patient's satisfaction.

Methods: Thirty seven denture wearers participated in study by filling out questionnaires about their satisfaction with dentures before and after relining. In that way the data indicating the subjective patient's experience and satisfaction were collected.