[The effectiveness of propranolol therapy of subfold hemangioma in the children during the first year of life].

The objective of the present study was to estimate the effectiveness and tolerance of propranolol therapy prescribed to the children presenting with vascular hyperplasia of the larynx. The experience with propranolol therapy of 12 patients suffering vascular hyperplasia of the larynx (subfold hemangioma) is analysed. Nine of these children had been given systemic glucocorticoid therapy prior to the present study that failed to produce a clinically significant effect.

[Species composition of yeast-like fungi isolated from HIV-infected patients].

The strains of yeast-like fungi isolated from HIV-infected people in 1994-2005 were examined. It was found that a share of non-albicans strains increased up to 46.2%, in monoculture they were present in 40% of examined patients, Candida glabrata (21.

[Antimycotic sensitivity of yeast-like fungi isolated from HIV-infected patients].

Sensitivity of museum and fresh strains to antimycotics has been determined. 5-Fluorocytosine (86.2%) and amphotericine B (93.

[Investigation of fatty acid composition for identification of clinical isolates of Pseudomonas spp].

The use of commercial test-systems has not allowed to identify 11 atypical clinical strains of nonfermenting Gram-negative bacteria. The divergences arised not only under their species identification, but also under generic one. On the basis of studying the fatty acid composition of the cell wall all isolates were referred to Pseudomonas genus.

[The colicynogenic activity and anthibiotic sensitivity of collected and newly isolated strains of Shigella flexneri and Shigella sonnei].

Among the collection strains of S. flexneri 10.5; 5.

[Property of collection strains and new isolates of Shigella sonnei].

The vital ability, serological properties, antibioticograms and biochemical properties of collection strains and new isolates of Shigella sonnei have been studied. It was ascertained that the determined factors are sufficiently variable, thus they cannot be used as the independent basis for the classification of pathogens.

[Change of serological properties of museum strains of Shigella flexneri in different terms of storage].

The viability, purity, biological and serological properties of museum strains of Shigella flexneri which were kept in lyophilized state during above 30 years were studied. Primary serological characteristics were specified, it was established that type 2a is the predominant serotype independent of the years and regions of isolation. Some strains lost antigens as a result of the long-term preservation or reinoculation on agar media.

[Comparative characteristics of physico-chemical and regulating properties of aspartate kinases from three species of cyanobacteria].

Aspartokinases have been isolated from the cell-free extracts of Plectonema boryanum, Anabaena variabilis and Synechococcus cedrorum. Their physico-chemical characteristics and peculiarities of retroregulation by amino acids were studied. It has been shown that in P.

[Use of associative cultures of cyanobacteria for tertiary treatment of sewage from yeast and alcohol plants].

The associative cultures of cyanobacteria were selected which may be used for development of technology of tertiary treatment of sewage for yeast and alcohol production plants. Growth parameters of associations were studied during periodic and continuous cultivation. When studying the influence of flowing speed for some exponents of treatment it was shown that cultivation of cyanobacteria should be carried out with the change of volume not more than 0.

[The identification of freshly isolated strains, the causative agents of human candidiasis and the search for effective antifungal probiotics].

The species variety of yeasts of the genus Candida in children and patients with AIDS has been investigated. Composition of the studied species was found to be similar in newborns and in women with candidiasis. In order to create new probiotics with antifungal properties, the cluster analysis of antagonistic activity of some Bacillus strains against Enterobacteriaceae, Candida, normoflora and food-fermenting lactobacteria has been conducted.

[Key enzymes of biosynthesis of amino acids of the glutamic series in the virus-cell system Anabaena variabilis + A-1].

The influence of cyanophage A-1 reproduction on glutamate dehydrogenase (GDG) and glutamine synthetase (GS) in A. variabilis cells was studied. It was determined that the both enzymes are intensified by 70% and 30%, accordingly.

[The effect of electromagnetic field on the biosynthesis and various properties of extracellular proteinase and lectin from Bacillus subtilis 316 M].

The promoting effect of electromagnetic field (EMF) on biosynthesis and activity of extracellular proteinase and lectin in B. subtilis 316 M was observed. It caused 1.

[Effect of reproduction of the LPP-3 cyanophage on glutamate dehydrogenase and glutamine synthetase activity in the cyanobacterium Plectonema boryanum].

The effect of cyanophage LPP-3 reproduction on glutamate dehydrogenase and glutamine synthetase (GS) in P boryanum cells have been studied. It was determined that the both reactions are intensified by 135% and 220%, accordingly. Isoenzymes of GS were purified from native and infected cell of cyanobacteria.

[Alanine dehydrogenase of the cyanobacterium Plectonema boryanum in the early period of cyanophage LPP-3 development].

It has been studied how reproduction of LPP-3 in Plectonema boryanum cells influences the alanine dehydrogenase activity. It has been found that immediately after the virus adsorption the enzyme activity falls by 50% and the anabolic reaction is blocked. Physicochemical properties of the enzyme vary as well.

[Aspartate kinase from the cyanobacteriium Plectonema boryanum infected with the cyanophage LPP-3].

The effect of development of cyanophage infection on the activity of aspartate kinase of Plectonema boryanum has been studied. It has been determined that activity of aspartate kinase increased during early period of reproduction of cyanophage. It coincided in time with the increase of the level of amino acids of aspartate family.

[Alanine dehydrogenase from the cyanobacterium Plectonema boryanum].

Alanine dehydrogenase activity of Plectonema boryanum cells was studied. It was found, that only NAD(H) may be used as the coenzyme. Kinetical parameters for substrates and coenzymes were determined.

[Aspartate kinase complex of Anabaena variabilis during the early period of development of cyanophage A-1].

Aspartate kinase activity in cells of A. variabilis has been studied in the dynamics of development of virus infection. An early period of reproduction of cyanophage A-1 has been determined to be conjugated with the increase of biosynthesis of amino acids from aspartate family.

[Aspartate kinase activity of Cyanobacteria Anabaena variabilis].

Aspartate-kinase (ASK) activity of filamentous cyanobacteria A. variabilis and its dependence on physico-chemical factors and substrate concentration in the reaction mixture have been studied. Three isoenzymes ASK-1, ASK-2 and ASK-3 which differ in the values of pH-optima, molecular weight, isoelectric points and effector of retro-inhibition have been isolated by ion-exchange chromatography.

[Isolation, purification and various properties of aspartate kinase from the cyanobacterium Plectonema boryanum].

The enzymic activity of aspartate kinase from filamentous cyanobacteria Plectonema boryanum at the logarithmic phase of growth has been studied. Aspartate kinase was purified eleven times and extracted in functionally homogeneous state from the cell-free extract by the methods of ammonium sulphate salting-out, ion-exchange chromatography and isoelectric focusing. Some physical and chemical characteristics of the enzyme have been studied: pI-7.

[Effect of an electromagnetic field on subtilisin from Bacillus subtilis 316 m].

The influence of electromagnetic field on the subtilisin of Bacillus subtilis strain 316 M preparation has been studied. It is the increase 30-60% of the proteolytic activity during 60-100 min treatment was observed. The effect of activation is stable for a month at +4 degrees C.

[Characteristics of hydrolase biosynthesis in Bacillus mesentericus grown on various media].

The dynamics of the consumption of major carbon and nitrogen sources and the biosynthesis of hydrolytic enzymes were studied in Bacillus mesentericus grown on semisynthetic media. Conditions were chosen that provide the obtaining of the culture liquid with predominantly proteolytic or amylolytic activity. The replacement of maltose with native starch resulted in more intensive accumulation of the biomass and hydrolytic enzymes, and in more rapid (by 3-5 hr) transformation from the logarithmic to the stationary growth phase.

[Hydrolase biosynthesis by morphological variants of Bacillus mesentericus as dependent on the storage conditions].

A method used to prepare the inoculum (a strain of Bacillus mesentericus, a producer of a complex of hydrolytic enzymes) has been studied for its effect on the activity of proteinases and amylases under submerged cultivation in fermenters. Optimal conditions for the culture storage and inoculum cultivation are developed to obtain standard enzymic preparations.

[Various physico-chemical properties of the amylolytic complex from Bacillus mesentericus].

Some physico-chemical properties of the Bacillus mesentericus amylolytic complex were studied, and optimal conditions of starch hydrolysis (pH 7.5-8.0; 45 degrees C) were found.

[Use of starch-containing media for cultivation of Bacillus mesentericus].

When cultivating Bacillus mesentericus to produce proteinases it is advisable to use more available and cheap carbon sources--native maize meal or potato starch--instead of maltose; the products of their complete hydrolysis inhibit the biosynthesis of enzymes.