Ascitic tumor cell cycle metabolism and energetics.

Data are presented on Ehrlich ascitic tumor cells energetic metabolism, activities of the glycolytic enzymes and the pentose phosphate pathway enzymes, contents and synthesis rates of the macromolecules at various cell cycle stages. An attempt was made to correct the direct measurement by taking into consideration a systematic error introduced in the experiment by incomplete cells synchronization. Cell metabolism activation sharply increased at two mitotic cycle stages.

Intracellular ratio between monovalent cations (Na+/K+), and transcriptional activity of genes in tumour cells.

The effects of the intracellular Na+/K+ ratio on transcriptional activity of ribosomal, c-fos, beta-actin and histone genes of Ehrlich ascites tumour cells and P-388 leukaemia cells have been investigated. Optimum values of the Na+/K+ ratio for selective transcription of genes are shown, they are in accordance with the Na+/K+ ratio and mRNA content of respective genes during the cell cycle. Differential activation and repression of these genes do not correlate with the total synthesis rate of RNA during the cell cycle.

[Effects of intracellular Na+/K+ ratio on expression of c-fos oncogene and beta-actin gene in ascitic cells of leukemia P-388 and Ehrlich tumor].

The intracellular Na+/K+ ratio was studied for its effect on expression gene beta-actin and oncogene c-fos and activity of these genes during the mitotic cycle in ascites cells of leukemia P-388 and Ehrlich tumour. It was established that gene beta-actin was activated at high and low ratios of Na+/K+, while c-fos only at high ones. The expression of these genes during the mitotic cell cycle was due to changes in the intracellular Na+/K+ ratio.

[Effects of intracellular Na+/K+ ratio on histone gene expression in ascitic cells of leukemia P-388 and Ehrlich cancer].

The intracellular Na+/K+ ratio was studied for its effect on histone genes expression in Ehrlich carcinoma and leukemia P-388 ascites cells. After equilibration with certain Na+/K+ ratio buffers at 0-4 degrees C the cells were treated during 1 h at 37 degrees C with ouabain added to the corresponding medium. Then total RNAs were extracted and analyzed by the reaction of blot-hybridization with histone genes cluster in plasmid p604.

[The effect of inhibitors of ionic passive transport on the structure of a cell population of Ehrlich's ascites carcinoma].

Triampur is a drug, containing triamterene and dichlotiazide, which inhibits passive influx of Na+ through cell membrane. Triampur was injected intraperitoneally to mice with the Ehrlich ascite carcinoma. The drug was injected in the exponential phase of tumor growth with 2 hour intervals within 24 hours.

[Use of the method of nucleus sedimentation fractionation for studying the dynamics of cell populations in the regenerating liver].

In different periods after partial hepatectomy (0, 11, 18, 24, 48 and 72 hours) isolated liver cell nuclei were separated according to their sizes using free sedimentation at 1 g in the exponential 0.1-1.0 M concentration gradient of sucrose at 4 degrees C.

[Analysis of the statistical characteristsics of the distribution of NK/Ly lymphoma cells obtained by laser flow cytometry].

Distributions in volumes of NK/Ly ascite lymphoma cells in different periods after inoculation were studied by laser cytometry. With the age of tumour the primary peak of distribution shifts to an area corresponding to large cells, but from the sixth day there appears an additional peak of distribution due to an accumulation of a pool of nonproliferating quiscent G0(R1) cells. The obtained data are in good agreement with results of sedimentation fractionation of NK/Ly lymphoma cells.

Isolation of low molecular protein P-9 from nuclei of NK/Ly lymphoma and Guerin carcinoma.

Protein with molecular weight 9000 (protein-9) was found in nuclei of resting cells of NK/Ly lymphoma and Guerin carcinoma by electrophoresis in polyacrylamide gel. Method of preparative isolation P-9 concluding in fractionation of nuclei from phenol extract by acetone at different pH and following electrophoresis in polyacrylamide gel was made. Electrophoretically homogeneous preparation of protein P-9 from nuclei of NK/Ly lymphoma and Guerin carcinoma was obtained.

[Isolation and physico-chemical properties of P-9 protein, marker for the resting cells of lymphoma NK/Ly and Gerene carcinoma].

A fraction of tumour cells in the state of proliferative rest was prepared by sedimentation from aged Ehrlich tumor and lymphoma NK/Ly. These cells do not incorporate [3H]-thymidine and contain fewer proteins and RNAs than the proliferating cells. The incorporation of [3H]-uridine by these cells makes up to 3.

[Cell population structure and nuclear macromolecular synthesis at different stages of the cell cycle in Guerin's carcinoma according to the data from the sedimentation fractionation of isolated nuclei].

For separating isolated nuclei of the Geren carcinoma, according to phases of the cell cycle, the sedimentation fractionation technique in steep gradients of sucrose at 1 g was used. Four peaks were displayed on a sedimentogram, corresponding to the four bands of sedimentating nuclei. From the results of DNA determination and of thymidine-3H incorporation in "pulse chase" experiments, the three peaks of the sedimentogram are identified as those corresponding to nuclei being in G1- and G2-periods of cycles of diploid and tetraploid tumour subpopulations, and ranges between the peaks are identified as nuclear fractions corresponding to S-periods of cycles of both the subpopulations.

[Lactate dehydrogenase and malate dehydrogenase isoenzymes at different stages of the mitotic cycle in Ehrlich tumor cells].

LDH is represented in the tumor almost exclusively by an extremely slow migrating isozyme LDH-5. There is actually no LDH-1, LDH-2, LDH-3 in the spectrum, while LDH-4 is in small amounts. MDH is represented by cytoplasmic isozyme (S-MDH) and a mitochondrial fraction (m-MDH).