[The use of synthetic glycoconjugates as components of the immunochromatographic test for rapid serological diagnosis of leprosy.].

The glycoconjugates with BSA (bovine serum albumin) were synthesized using a next saccharide: disaccharide derivative M.leprae PGL-1 (phenolic glycolipid-1); a complex of the disaccharide fragment and the branched hexasaccharide fragment LAM (lipoarabinomannan); diarabinofuranose fragment LAM. These glycoconjugates were used as antigenic components for leprosy rapid serotest construction in immunochromatographic format (leprosy LF serotest).

Two Isoforms of Yersinia pestis Plasminogen Activator Pla: Intraspecies Distribution, Intrinsic Disorder Propensity, and Contribution to Virulence.

It has been shown previously that several endemic Y. pestis isolates with limited virulence contained the I259 isoform of the outer membrane protease Pla, while the epidemic highly virulent strains possessed only the T259 Pla isoform. Our sequence analysis of the pla gene from 118 Y.

Rational design of antigens to improve the serodiagnosis of tick-borne borreliosis in central regions of Russia.

Tick-borne borreliosis (Lyme disease-LD) is caused by pathogenic Borrelia spirochetes that is transmitted through bite of Ixodes ticks to humans and animals. In the Russian Federation, borreliosis registered with an index of 6-7 per 100,000 people annually. In reality, LD morbidity in Russia is much higher because Russian strains develop less erythematous rashes compared to North American strains, thus missed by physicians in most of the early cases, and current serology tests have insufficient sensitivity as well.

[Biosafety provision on handling pathogenic biological agents on the concept of biorisk assessment and management].

The paper shows it urgent to realize the concept of biological risk assessment and management on handling pathogenic biological agents (PBA). It gives a number of objective reasons that impede development of a methodology to assess laboratory biological risks. A concept of continuous improvement (a process approach) is proposed for use as a biorisk management tool for biosafety assurance when handling PBA.

[Isolation, identification, and characteristics of the phytotoxin produced by the fungus Alternaria cirsinoxia].

An individual substance (20 mg/l) exhibiting phytotoxic properties, which, on the basis its spectral characteristics, was identified as zinniol, was obtained from the fungus Alternaria cirsinoxia. The nonspecific activity of this phytotoxin, with respect to plants of different families, was demonstrated. The minimum concentration (200 microg/ml) at which zinniol damages creeping thistle leaves and the median inhibition concentration (IC50) for rat embryonic fibroblasts (264 microg/ml) were determined.

[Microfungicid--a preparation based on trichoderma viride for plant diseases control].

A technology was designed for manufacturing a preparation based on Trichoderma viride Pers ex S.F. Gray that strongly suppresses the development of causative agents of certain plant diseases and displays a growth-stimulating activity.

[Comparative study of the changes in lactate dehydrogenase isoenzymatic spectra in the course of organogenesis in mice].

The dynamics of changes in the lactate dehydrogenase (LDH) spectra during organogenesis in CBA mice has been studied by means of ultramicroelectrophoresis. The embryonic period of development is characterized by the predominance of cathodic isozymes (LDH-5 and LDH-4) in all the organs under study. The increase of anodic isozymes (LDH-1 and LDH-2) takes place in the heart, kidneys and brain as the development proceeds.

[Repression and derepression of lactate dehydrogenase loci during mouse development].

The ultramicroelectrophoretic method was applied to the study of the lactic dehydrogenase (LDH) spectrum alterations during the developmental phases of mice: ovulated ova--zygote--blastocyte--embryo--oocyte--ovulated ova. Only H-subunits were found in the embryo cells before the 5th day of development. After this M-subunits appeared indicating derepression of LDH-A locus.