Subunits Med12 and Med13 of Mediator Cooperate with Subunits SAYP and Bap170 of SWI/SNF in Active Transcription in .

SAYP and Bap170, subunits of the SWI/SNF remodeling complex, have the ability to support enhancer-dependent transcription when artificially recruited to the promoter on a transgene. We found that the phenomenon critically depends on two subunits of the Mediator kinase module, Med12 and Med13 but does not require the two other subunits of the module (Cdk8 and CycC) or other subunits of the core part of the complex. A cooperation of the above proteins in active transcription was also observed at endogenous loci, but the contribution of the subunits to the activity of a particular gene differed in different loci.

Optimization strategies and advances in the research and development of AAV-based gene therapy to deliver large transgenes.

Adeno-associated virus (AAV)-based therapies are recognized as one of the most potent next-generation treatments for inherited and genetic diseases. However, several biological and technological aspects of AAV vectors remain a critical issue for their widespread clinical application. Among them, the limited capacity of the AAV genome significantly hinders the development of AAV-based gene therapy.

Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in .

The chromatin remodeler SWI/SNF is an important participant in gene activation, functioning predominantly by opening the chromatin structure on promoters and enhancers. Here, we describe its novel mode of action in which SWI/SNF factors mediate the targeted action of an enhancer. We studied the functions of two signature subunits of PBAP subfamily, BAP170 and SAYP, in .

[The effect of different methods of photohemotherapy on the rheological properties of the blood in patients with ischemic heart disease].

The authors made an analysis of results of examination of 41 patients with ischemic heart disease treated by the standard medicamentous therapy and when using different methods of photohemotherapy against its background. It was established that medicamentous therapy during 2 weeks failed to result in a substantial improvement of rheological properties of blood, while its combination with photohemotherapy could give a considerable positive effect coinciding with clinical improvement of the patient's state. Shorter terms are required to correct hemorheological indices when autotransfusions of photomodified blood are used.

[The significance of the association of erythrocytic antigens in rheumatism].

A study is presented of ABO system, rhesus, MN, P, Lewis factor in 78 patients with rheumatism. Taken separately their information value was low. At the same time in patients with O (I), A (II), B (III) blood groups association of antigens were often met that allows to isolate among healthy persons groups of high risk as related to rheumatism with the purpose of realizing primary prophylaxis.

[An experimental infection of the seal Phoca sibirica with the morbilli virus].

A Baikal seal (Phoca sibirica) was experimentally infected with Baikal seal morbillivirus (BSMV) isolated from Baikal seals during an epizootic in 1987-1988. The seal was infected with BSMV with an infectious titer of 10(7.0) TCD50/ml, and daily observations of the animal clinical condition were made.

[Injuries of the heart].

The authors had 53 patients with heart injuries under observation; 52 patients were operated on for vital indications. The heart wound was sutured, the concomitant injuries to other organs were removed, and the pericardial and pleural cavities were drained. Air-tightness of the injured heart muscle was attained in all cases.

[Traumatic injuries and diseases of the diaphragm].

The article analyses experience in diagnosing the pathological condition and treating 125 patients with acute injury to the diaphragm (61 patients) and chronic traumatic diaphragmatic hernias (64 patients). The authors indicate the therapeutic and diagnostic means in this pathological emergency due to the acute trauma of the chest and abdomen or incarceration of the abdomen. The diagnostic errors and their consequences are analysed.

[The isolation of the morbilli virus from the Baikal seal Phoca sibirica and its preliminary characterization].

A morbillivirus was isolated from the organs of a seal (Phoca sibirica) which had died during 1987-1988 epizootic in Baikal. This Baikal seal morbillivirus (BSM) was adapted to Vero cell cultures in which it induced a cytopathic effect developing to complete destruction of the monolayer. Typing of BSM was done by indirect immunofluorescence test and enzyme immunoassay using antibodies to distemper and measles viruses.

[Experimental anthrax infection in laboratory animals with differing species susceptibility to the causative agent].

In guinea pigs and noninbred white mice, infected subcutaneously with anthrax which resulted in their death, characteristic generalized infection with the hematogenic contamination of their organs and the signs of intoxication and shock could be observed. In inbred white rats (Fisher 344) the invasion and dissemination of B. anthracis are relatively slightly pronounced, the phenomena of intoxication and shock being clearly prevalent.

[Specific intoxication in anthrax infection].

The pathomorphological picture of experimental B. anthracis infection in white rats (strain Fisher-344) essentially corresponds to experimental anthracic intoxication with very moderately pronounced morphological manifestation of B. anthracis invasion.

[Anthrax toxin dynamics in the body].

Anthrax toxin introduced subcutaneously into white rats Fisher-344 caused specific intoxication accompanied by characteristic pathomorphological changes and, when studied by the immunoperoxidase method in histological sections, could be detected in the blood stream (in plasma) and in the cytoplasm of macrophages (in the lungs and the spleen). The content of the preparation introduced into the animals in the blood stream is directly related to its toxicity, being probably indicative of the relative functional failure of the macrophagal system.

[Microelement composition of the hair of workers in the ceramic paint industry].

By means of atomic absorption spectrophotometry, the neutron-activation analysis and chemicospectral procedure the content of 33 trace elements in the hair samples of 48 workers of various professions at the shop of ceramic paints was analyzed. Elevated cadmium and lead concentrations were identified in the air. Increased concentrations of lead, cadmium, tellurium, arsenic, chromium, cobalt, antimony, silver, vanadium, manganese, selenium, aluminum, titanium were also identified in workers' hair.

[Experimental characteristics of anthrax intoxication].

In experimental anthrax intoxication, a highly important stage of its pathogenesis consists in microcirculatory disturbances with the phenomena of blood sludge, accompanied by the increased permeability of blood vessels not only for plasma, but also for red blood cells. These disturbances result in perivascular hemorrhages, hemorrhagic infiltrations, edema and cavitary transudates. Pulmonary edema and, as a consequence, the accumulation of fluid in pulmonary alveoli and the respiratory tract are of particular importance and, probably, can be considered the basic cause of the ensuing acute and fatal asphyxia.