Publications by authors named "Kolbasova O"

Article Synopsis
  • The study examined a caprine arthritis encephalitis (CAE) virus isolate from goats in Mordovia, Russia, detected through blood monitoring for lentivirus presence.
  • The positive PCR result was confirmed by nucleotide sequencing, revealing that the isolate shares a genetic similarity with other small ruminant lentiviruses in the GenBank database.
  • Phylogenetic analysis placed the "Mordovia-2018" isolate in the same cluster as a previously identified isolate from Tver region in 2008, and the study detailed the successful isolation of the virus using blood monocyte cells in a lamb cell culture.
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African swine fever virus (ASFV) is an extremely genetically and phenotypically heterogeneous pathogen. Previously, we have demonstrated that experimental inoculation of pigs with an attenuated strain, Katanga-350 (genotype I, seroimmunotype I) (ASFV-Katanga-350), can induce protective immunity in 80% of European domestic pigs against the homologous virulent European strain Lisbon-57. At least 50% of the surviving pigs received protection from subsequent intramuscular infection with a heterologous virulent strain, Stavropol 01/08 (genotype II, seroimmunotype VIII) (ASFV-Stavropol 01/08).

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The African swine fever virus (ASFV) is the cause of a recent pandemic that is threatening the global pig industry. The virus infects domestic and wild pigs and manifests with a variety of clinical symptoms, depending on the strain. No commercial vaccine is currently available to protect animals from this virus, but some attenuated and recombinant live vaccine candidates might be effective against the disease.

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African swine fever virus causes hemorrhagic disease in swine. Attenuated strains are reported in Africa, Europe, and Asia. Few studies on the diagnostic detection of attenuated ASF viruses are available.

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The facultative intracellular pathogen is of major veterinary importance in small ruminants. Nevertheless, details of interactions with cells of small ruminants are not fully established. To study the potential of to infect sheep cells, we used the finite sheep kidney cell line (shKEC), which was infected with the wild-type strain EGDe.

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Introduction: Bats are an epidemiologically important natural reservoir of viruses of various taxonomic groups, including causative agents of especially dangerous infections of humans and animals. Considering the relevance of arbovirus infections, it seems advisable to study the spectrum of the sensitivity of cells derived from bats inhabiting and migrating on the territory of the Russian Federation to causative agents of vector-borne diseases of animals.The study aimed to obtain a diploid strain of cells from renal tissue of bats Pipistrellus nathusii and to investigate its biological characteristics, as well as to assess its permissiveness for bluetongue (BTV); Rift Valley fever (RVFV); lumpy skin disease (LSDV); rabbit myxoma (Myxomatosis cuniculi); rabbit, or Shope fibroma (RFV); African horse sickness (AHSV) and African swine fever (ASFV) viruses.

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is a widespread facultative intracellular pathogen. The range of natural hosts that supporting persistence in the environment has not been fully established yet. In this study, we were interested in the potential of to infect cells of bats, which are being increasingly recognized as a reservoir for microorganisms that are pathogenic to humans and domestic animals.

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The infections caused by small ruminant lentiviruses include diseases, such as Maedi-Visna (MV) and caprine arthritis-encephalitis (CAE). According to phylogenetic findings and their common origination, small ruminant lentiviruses were divided into Groups A, B, C, D, and E. Cultivation of the lentiviruses displayed the cytopathic effect of the CAE virus strain 75 G-63 in the primary culture of goatling synovial membrane cells, which was shown by monolayer destruction and polynuclear cell formation; this was uncharacteristic for M-88, K-796, and Tverskoy strains.

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