A Combination of Distinct Vascular Stem/Progenitor Cells for Neovascularization and Ischemic Rescue.

Background: Peripheral vascular disease remains a leading cause of vascular morbidity and mortality worldwide despite advances in medical and surgical therapy. Besides traditional approaches, which can only restore blood flow to native arteries, an alternative approach is to enhance the growth of new vessels, thereby facilitating the physiological response to ischemia.

Methods: The Actin/R26 Rainbow reporter mouse was used for unbiased in vivo survey of injury-responsive vasculogenic clonal formation.

The Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans.

Molecular characterization of cell types using single-cell transcriptome sequencing is revolutionizing cell biology and enabling new insights into the physiology of human organs. We created a human reference atlas comprising nearly 500,000 cells from 24 different tissues and organs, many from the same donor. This atlas enabled molecular characterization of more than 400 cell types, their distribution across tissues, and tissue-specific variation in gene expression.

Engaging Pharmacists to Crowdsource a Fine-grained Medication Risk Scale: An Initial Measurement Study Using Paired Comparisons of Medications.

A coarse classification of medications into two risk categories, one for high-risk medications and one for all others, allows people to focus safety improvement work on medications that carry the highest risks of harm. However, such coarse categorization does not distinguish the relative risk of harm for the majority of medications. To begin to develop a more fine-grained measurement scale for the relative risk of harm spanning many medications, we performed an experiment with 18 practicing pharmacists.

Hybrid bag of approaches to characterize selection criteria for cohort identification.

Objective: The 2018 National NLP Clinical Challenge (2018 n2c2) focused on the task of cohort selection for clinical trials, where participating systems were tasked with analyzing longitudinal patient records to determine if the patients met or did not meet any of the 13 selection criteria. This article describes our participation in this shared task.

Materials And Methods: We followed a hybrid approach combining pattern-based, knowledge-intensive, and feature weighting techniques.

Telomere shortening is a hallmark of genetic cardiomyopathies.

This study demonstrates that significantly shortened telomeres are a hallmark of cardiomyocytes (CMs) from individuals with end-stage hypertrophic cardiomyopathy (HCM) or dilated cardiomyopathy (DCM) as a result of heritable defects in cardiac proteins critical to contractile function. Positioned at the ends of chromosomes, telomeres are DNA repeats that serve as protective caps that shorten with each cell division, a marker of aging. CMs are a known exception in which telomeres remain relatively stable throughout life in healthy individuals.