Repeated immunosensing by a dithiobis(succinimidyl propionate)-modified SAW device.

The possibility of dithiobis(succinimidyl propionate) (DSP) for repeated immunoassay with a surface acoustic wave (SAW) immunosensor was explored. In the sensor, DSP was used to modify a gold-coated quartz sensing area of a SAW device by forming a self-assembled monolayer on the gold surface. In a model sandwich assay using mouse (mIgG) and anti-mouse (a-mIgG) antibodies, the primary antibody, mIgG, firstly reacted with N-hydroxysuccinimide ester groups of DSP and was immobilized on the SAW device to fabricate the SAW immunosensor.