Publications by authors named "Koki Amano"
In influenza A virus-infected cells, newly synthesized viral neuraminidases (NAs) transiently localize at the host cell Golgi due to glycosylation, before their expression on the cell surface. It remains unproven whether Golgi-localized intracellular NAs exhibit sialidase activity. We have developed a sialidase imaging probe, [2-(benzothiazol-2-yl)-5-(non-1-yn-1-yl) phenyl]-α-D-N-acetylneuraminic acid (BTP9-Neu5Ac).
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- The study examined the decline of Comamonas testosteroni R2's ability to degrade phenol in chemostat conditions and its aerobic growth in batch settings, identifying physiological and transcriptomic changes.
- It was found that the accumulation of metabolites led to decreased enzymatic activities for phenol and catechol degradation and a significant up-regulation of genes for nitrogen transport, particularly ammonium uptake, under collapse conditions.
- The research demonstrated that amino acids like L-arginine can reverse growth inhibition by balancing carbon and nitrogen metabolism, indicating that this imbalance contributed to the strain's collapse.
The coexisting mechanism of a synthetic bacterial community (SBC) was investigated to better understand how to manage microbial communities. The SBC was constructed with three kinds of phenol-utilizing bacteria, Pseudomonas sp. LAB-08, Comamonas testosteroni R2, and Cupriavidus sp.
View Article and Find Full Text PDFDraft Genome Sequence of Phenol-Degrading Variovorax boronicumulans Strain c24.
Microbiol Resour Announc
September 2020
We report the draft genome sequence of strain c24, which was isolated from a soil-inoculated chemostat culture amended with phenol as a sole carbon and energy source. The genome data will provide insights into phenol and other xenobiotic compound degradation mechanisms for bioremediation applications.
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