Publications by authors named "Kohsaku Numa"
Article Synopsis
- The study investigates whether polishing can remove guttae in Fuchs endothelial corneal dystrophy (FECD) without needing Descemet stripping and if cultured corneal endothelial cell (CEC) transplants can help maintain reduced guttae post-surgery.
- A case series conducted at Kyoto Prefectural University Hospital followed 22 eyes over three years, focusing on changes in guttae after cultured CEC transplant therapy.
- Results showed that all 15 monitored eyes had guttae pre-op, with a notable reduction in guttae presence after surgical intervention, indicating that both procedures can effectively reduce guttae.
Article Synopsis
- The study aimed to investigate how UV-A radiation affects the aging characteristics (senescent phenotypes) of human corneal endothelial cells (hCEnCs).
- Researchers analyzed various factors like cell shape, growth rates, and specific aging markers in hCEnCs treated with UV-A, comparing results to those from cells subjected to ionizing radiation (IR).
- Findings showed significant overlap in gene and protein changes between UV-A and IR-induced senescent hCEnCs, indicating UV-A triggers similar aging responses while also influencing unique pathways.
Purpose: The ocular surface microenvironment changes with aging. However, it remains unclear if cellular senescence influences the ocular surface. We investigated the presence of p16-expressing senescent cells in healthy human conjunctiva.
View Article and Find Full Text PDFPurpose: This study aimed to investigate the senescent phenotypes of human corneal and conjunctival epithelial cells.
Methods: We examined cell morphology, senescence-associated β-galactosidase (SA-β-gal) activity, cell proliferation, and expression of senescence markers (p16 and p21). RNA sequencing analysis was conducted to compare gene expression profiles between senescent and non-senescent cells.
Article Synopsis
- Aging can cause cells in the eye to become "senescent," which means they stop working properly and can lead to diseases.
- When these cells age, they secrete harmful substances that can damage the eye's protective barrier, making things worse over time.
- In a study, old mice showed worse eye problems than younger ones, but treating them early helped prevent some of these issues, suggesting that getting rid of senescent cells might help our eyes stay healthier as we age.
Purpose: Dry eye disease (DED) is a common age-related ocular surface disease. However, it is unknown how aging influences the ocular surface microenvironment. This systematic review aims to investigate how the aging process changes the ocular surface microenvironment and impacts the development of DED.
View Article and Find Full Text PDFPurpose: To investigate the safety and efficacy of cultured human corneal endothelial cell (hCEC) injection therapy with mature differentiated (mature) cell subpopulations (SPs) for corneal endothelial failure (CEF).
Design: Comparative, interventional case series.
Methods: This study involved 18 eyes with CEF that underwent cultured hCEC injection therapy, categorized into 2 groups: (1) 11 eyes administered a relatively lower proportion (0.
Mitochondria as a Platform for Dictating the Cell Fate of Cultured Human Corneal Endothelial Cells.
Invest Ophthalmol Vis Sci
December 2020
Purpose: Aiming to clarify the role of mitochondria in cell fate decision of cultured human corneal endothelial cell (cHCEC) subpopulations.
Methods: The mitochondrial respiratory ability were examined with Mito stress and Mito fuel flex test assays using an extracellular flux analyzer (XFe24; Agilent Technologies; Santa Clara, CA) for human corneal endothelium tissues, mature cHCECs and a variety of cell state transitioned cHCECs. Tricarboxylic acid cycle and acetyl-coenzyme A-related enzymes was analyzed by proteomics for cell lysates using liquid chromatography-tandem mass spectrometry for cHCEC subpopulations.
Purpose: To report the safety and efficacy of a novel cell injection therapy using cultured human corneal endothelial cells (hCECs) for endothelial failure conditions via the report of the long-term 5-year postoperative clinical data from a first-in-humans clinical trial group.
Design: Prospective observational study.
Participants: This study involved 11 eyes of 11 patients with pseudophakic endothelial failure conditions who underwent hCEC injection therapy between December 2013 and December 2014.
Purpose: To clarify the expression profiles of ion channels and transporters of metabolic substrates among heterogeneous cultured human corneal endothelial cells (cHCECs) distinct in their effectiveness in reconstituting the corneal endothelium.
Methods: Integrated proteomics for cell lysates by liquid chromatography-tandem mass spectrometry was carried out from three aliquots of cHCECs enriched in either cluster of definition (CD)44-/+ (mature) cHCECs or CD44++/+++ cell-state transition (CST) cHCECs. The expression profiles of cations/anions, monocarboxylic acid transporters (MCTs), and solute carrier (SLC) family proteins, as well as carbonic anhydrases (CAs), were investigated.
Metabolites Interrogation in Cell Fate Decision of Cultured Human Corneal Endothelial Cells.
Invest Ophthalmol Vis Sci
February 2020
Purpose: Aiming to clarify the metabolic interrogation in cell fate decision of cultured human corneal endothelial cells (cHCECs).
Methods: To analyze the metabolites in the culture supernatants (CS), 34 metabolome measurements were carried out for mature differentiated and a variety of cHCECs with cell state transition through a facility service. Integrated proteomics research for cell lysates by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was performed for 3 aliquots of each high-quality or low-quality cHCEC subpopulations (SP).