Publications by authors named "Kohei Umehara"
The stress-induced 70 kDa heat shock protein (Hsp70) functions as a molecular chaperone to maintain protein homeostasis. Hsp70 contains an N-terminal ATPase domain (NBD) and a C-terminal substrate-binding domain (SBD). The SBD is divided into the β subdomain containing the substrate-binding site (βSBD) and the α-helical subdomain (αLid) that covers the βSBD.
View Article and Find Full Text PDFArticle Synopsis
- TALEN (Transcription Activator-Like Effector Nuclease) is a popular tool for genome editing, utilizing a series of TAL-repeats that recognize specific DNA bases through unique mutations.
- A modified version of TALEN, called VT-TALE, has mutations at non-RVD positions, which enhances its effectiveness in genome editing by improving target recognition and specificity compared to the standard CT-TALE.
- Research shows that VT-TALE exhibits greater superhelical motion, facilitated by altered hydrogen bonding between TAL-repeats, leading to better engagement with target DNA sequences.