Publications by authors named "Kohei Nishikaku"
Background: The Kumamoto strain of Japanese Brown (JBRK) cattle is a sub-breed of Wagyu and has a different genetic background than that of Japanese Black (JB) cattle. Bovine leukemia virus (BLV) is the pathogen causing enzootic bovine leukosis (EBL), the predominant type of bovine leukosis (BL). EBL is one of the most common bovine infectious diseases in dairy countries, including Japan.
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- Bovine leukemia virus (BLV) causes the growth of infected B lymphocytes and up to 5% of infected cows can develop enzootic bovine leukosis (EBL), which involves tumor cell proliferation.
- Using viral integration site analysis through a DNA capture-sequencing method, researchers discovered that identical tumor clones can be found in both blood and tumor tissue, suggesting that these cancer cells can circulate within the bodies of EBL-infected cattle.
- A new method called rapid amplification of integration sites (BLV-RAIS) was developed to efficiently diagnose EBL by detecting clonal expansion of BLV-infected cells, showing high diagnostic sensitivity and specificity, thus providing a reliable tool for early detection of EBL
Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is currently one of the most important pathogens affecting the cattle industry worldwide. Determining where and in which host it originated, and how it dispersed across continents will provide valuable insights into its historical emergence as the cattle pathogen. Various species in the genus were domesticated in Asia, where they also diversified.
View Article and Find Full Text PDFBovine leukemia virus (BLV) is an important pathogen associated with enzootic bovine leukosis. In this study, we performed PCR and sequencing analysis to characterize BLVgp51 sequences from formalin-fixed paraffin-embedded (FFPE) specimens made from 1974 to 2000 and successfully obtained BLV proviral genome sequences from 94% of the analyzed samples. Furthermore, from these samples, we reconstructed eight full-length and nearly full-length BLVgp51 sequences.
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- Bovine leukemia virus (BLV) causes enzootic bovine leukosis (EBL) and has a complicated relationship with the host genome, which isn't fully understood yet.
- A new DNA-capture-seq technique was developed to better understand how BLV integrates into the host genome and to identify clonal expansions of infected cells.
- The study found that BLV integration is prevalent in EBL tumor samples, highlighting the potential of this method for revealing the mechanisms behind BLV infection and its progression in cattle.
Bovine leukemia virus (BLV) is a causative agent of enzootic bovine lymphoma (EBL). BLV is prevalent worldwide, and ten genotypes have been classified based on the sequence of the envelope glycoprotein (gp51) gene. In this study, we present a simple and generally applicable PCR restriction fragment length polymorphism (PCR-RFLP) method to identify BLV genotypes.
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