Publications by authors named "Kohei Miyata"

Background/aim: Age-related decline in the number of ovulations and ovum quality are major causes of female infertility, and stem cells have been reported to be effective in tissue regeneration. However, current therapeutic modalities are inadequate. This study investigated the effects of adipose-derived mesenchymal stem cells (ASCs) on ovarian functions in aged mice.

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Many countries, including Japan, are experiencing declining birth rates. Assisted reproductive technologies have consistently demonstrated good results in resolving infertility. Although the development of fertilized eggs into blastocysts has been recognized as a crucial step in assisted reproductive technologies, the involved mechanisms are currently unclear.

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The purpose of our research is to develop functional additives that enhance mucosal absorption of biologics, such as peptide/protein and antibody drugs, to provide their non-to-poor invasive dosage forms self-managed by patients. Our previous in vivo and in vitro studies demonstrated that the intranasal absorption of biologics in mice was significantly improved when coadministered with oligoarginines anchored chemically to hyaluronic acid via a glycine spacer, presumably through syndecan-4-mediated macropinocytosis under activation by oligoarginines. The present mouse experiments first revealed that diglycine-L-tetraarginine-linked hyaluronic acid significantly enhanced the intranasal absorption of sulpiride, which is a poor-absorptive organic compound with a low molecular weight.

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  • A study aimed to enhance postpartum hemorrhage (PPH) management by examining how obstetric history and ultrasound findings can predict retained products of conception (RPOC) in patients with severe PPH.* -
  • The research analyzed data from 56 patients, using logistic regression to identify predictors of severe PPH, focusing on factors like second-trimester miscarriage, clinical suspicion timing, and ultrasound measurements.* -
  • Results indicated that ultrasound measurement (long-axis SIZE) was a significant predictor of severe PPH, and combining various predictive factors improved the accuracy of identifying patients at risk, suggesting a potential clinical screening tool.*
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  • This study investigates the lifespan and ideal concentration of ultrafine bubbles (UFBs) for their use in biological applications, noting their promising properties.
  • The research utilized a generator to create UFBs and measured their size and concentration over time, finding a decrease in concentration of about 30% in the first two weeks but stability up to 6 months.
  • Results indicated that UFBs could be effectively generated for cell culture systems, as their concentration and size can be controlled and they significantly influence cell properties.
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  • Previous research showed that L-octaarginine linked to hyaluronic acid can improve the absorption of protein drugs under 22 kDa when taken intranasally.
  • This study explored its use for larger antibody drugs, showing that the bioavailability of ranibizumab (48 kDa) increased from 0.67% to 6.2% when combined with the hyaluronic acid derivative.
  • Results indicated that this absorption enhancement mechanism relies on syndecan-4-induced macropinocytosis, making it effective for various antibody sizes, with no involvement from clathrin or caveolin-1 in the uptake process.
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Hypoxia-inducible factor 1α (HIF1α) is a transcription factor that regulates angiogenesis under hypoxic conditions. To investigate the posttranscriptional regulatory mechanism of HIF1α, we performed a cell-based screening to reveal potential cis-elements and the regulatory RNA-binding proteins that act as trans-factors. We found that LIN28A promoted HIF1α protein expression independently of the downregulation of microRNA let-7, which is also directly mediated by LIN28A.

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  • - The study investigates how cell-penetrating peptides attached to polymers can enhance the absorption of biologics through mucosal routes, highlighting past mouse experiments that showed significant improvements in mucosal absorption of protein drugs using hyaluronic acid modified with l-octaarginine.
  • - Results revealed that somatropin had a bioavailability of 19% when applied to lung mucosa but only about 1% with nasal administration, which increased to 46% when paired with the absorption enhancer.
  • - The effectiveness of the absorption enhancer varied by route; while oral and rectal administration saw improved somatropin absorption, intragastric use did not yield positive results, suggesting the pulmonary route is optimal for administering biologics.
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  • * Researchers tested participants at various breathing rates (7.5 to 120 breaths per minute) and found that at lower rates, movements were synchronized in a stable pattern, while higher rates led to different coordination patterns.
  • * The results indicate that while thoracic and abdominal movements are closely linked at slower frequencies, their coordination can vary significantly with increased breathing rates, highlighting the need to understand breath control in a coordinated manner.
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Endometriosis, which exhibits enigmatic pathological features such as stromal fibrosis and proliferation of ectopic epithelial cells, is known as a refractory disease. Mesenchymal stem cells modulate the fibrosis in stromal tissues through their trophic and immunomodulatory properties. To investigate the potential of stem cells in treating endometriosis, we examined the secondary morphology and molecular alterations in endometriosis-like lesions after the administration of adipose tissue-derived stem cells (ASCs) to an experimental murine model of endometriosis.

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Temporal prediction ability is vital for movement synchronization with external rhythmic stimuli (sensorimotor synchronization); however, little is known regarding individual variations in temporal prediction ability and its neural correlates. We determined the underlying neural correlates of temporal prediction and individual variations during auditory-motor synchronization. We hypothesized that the non-primary motor cortices, such as the premotor cortex and supplementary motor area, are the key brain regions that correlate individual variations in prediction ability.

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Methamphetamine (METH), which is used to improve the alertness of narcoleptic patients, elicits autonomic physiological responses such as increases in body temperature, blood pressure and heart rate. We have shown that orexin synthesizing neurons, which have an important role in maintaining wakefulness, greatly contribute to the regulation of cardiovascular and thermoregulatory function. This regulation is partly mediated by glutamatergic as well as orexinergic signalling from the orexin neurons.

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Cell-penetrating peptides such as oligoarginines are one of promising tools that improve mucosal absorption of poorly membrane-permeable biologics. We have already demonstrated that conjugation of L-octaarginine to hyaluronic acid via a tetraglycine spacer resulted in a 3-fold enhancement of nasal absorption of somatropin (Mw: ca. 22.

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We have developed an aggregate of D-octaarginine immobilized at multiple points on a co-polymer of N-vinylacetamide and acrylic acid. Previous studies revealed that immunoglobulin G and A were induced when mice were inoculated with influenza virus antigens under coadministration with the D-octaarginine-immobilized polymers as a mucosal vaccine adjuvant. Infection experiments demonstrated that mice vaccinated with a mixture of inactivated influenza viruses and the polymers were protected from infection with mouse-adapted infectious viruses.

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  • Intra-amniotic infection (IAI) is linked to preterm birth and poor outcomes, prompting a study on vaginal microbiota as a risk assessment tool for chorioamnionitis (CAM) in preterm labors.
  • The study analyzed samples from 83 pregnant women, identifying two groups: those with CAM and those without, using a random forest algorithm to develop a predictive CAM scoring method (PCAM).
  • Results showed that the CAM group had greater microbial diversity and experienced shorter gestational periods, with a higher risk of developmental disorders in infants at age three compared to the non-CAM group.
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Introduction: Clinical prediction of foetal inflammatory response syndrome (FIRS) is highly necessary. We have previously reported that miR-4535 and miR-1915-5p are potential biomarkers for severe chorioamnionitis based on the results of microRNA array analysis. Therefore, we evaluated the relationship between foetal morbidity of infection and miR-4535, miR-1915-5p, interleukin (IL)-6, or 16S rDNA copy number levels in amniotic fluid from pregnant women with chorioamnionitis.

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Background: This study was performed to investigate the clinical significance of miR-4535 and miR-1915-5p in severe chorioamnionitis.

Materials & Methods: Amniotic fluid samples from 37 patients with severe chorioamnionitis were subjected to miRNA array analysis and ddPCR™. Diagnostic values were assessed using the receiver operating characteristic curve.

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Face-to-face imitation is a unique social interaction wherein a shared action is executed based on the feedback of the partner. Imitation by the partner is the feedback to the imitatee's action, resulting in sharing actions. The neural mechanisms of the shared representation of action during face-to-face imitation, the core of inter-subjectivity, are not well-known.

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Let-7 is an evolutionary conserved microRNA that mediates post-transcriptional gene silencing to regulate a wide range of biological processes, including development, differentiation, and tumor suppression. Let-7 biogenesis is tightly regulated by several RNA-binding proteins, including Lin28A/B, which represses let-7 maturation. To identify new regulators of let-7, we devised a cell-based functional screen of RNA-binding proteins using a let-7 sensor luciferase reporter and identified the tRNA pseudouridine synthase, TruB1.

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Because work songs are ubiquitous around the world, singing while working and performing a task with a coactor is presumably beneficial for both joint action and individual task performance. The present study investigated the impact of interpersonal rhythmic vocal interaction on interpersonal phase relations and on individual motor timing performance, which was evaluated by a synchronization-continuation paradigm requiring whole-body movement with or without visual contact. Participants repeated the syllable "" or remained silent in a manipulation of vocal interaction, and they were oriented toward or away from their partner to manipulate visual interaction.

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Our previous mouse studies demonstrated that mean bioavailability of exendin-4, which is an injectable glucagon-like peptide-1 (GLP-1) analogue whose molecular weight (Mw) and isoelectric point (pI) are ca. 4.2 kDa and 4.

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A rare case of palpebral cellulitis with simultaneous frontal sinusitis and osteomyelitis is reported. A healthy 45-year-old man presented with left upper eyelid swelling. He was given intravenous meropenem at the local hospital, but he failed to improve.

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Affective communication, communicating with emotion, during face-to-face communication is critical for social interaction. Advances in artificial intelligence have made it essential to develop affective human-virtual agent communication. A person's belief during human-virtual agent interaction that the agent is a computer program affects social-cognitive processes.

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Mucosal vaccination, which secretion of immunoglobulin A (IgA) on the mucosa is accompanied by induction of immunoglobulin G (IgG) in the blood, is one of the most effective ways to circumvent influenza epidemics caused by incorrect prediction of epidemic viral strains or viral mutation. Secreted IgA is expected to prevent hosts from being infected with heterologous viruses because this antibody cross-reacts to strains other than those used for immunization. Our previous mouse experiments revealed that intranasal IgA with cross-reactivity was induced through nasal inoculation with inactivated whole viral particles of the H1N1 A/New Caledonia/20/99 IVR116 (NCL) strain in the presence of hyaluronic acid modified with tetraglycine-l-octaarginine.

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Background/aim: Initial treatment of endometrial cancer with surgery and platinum and taxane-based chemotherapy is often successful, but it remains unclear as to whether certain types of the disease relapse. The aim of this study was to identify the clinical features of recurrence in patients without residual tumour in endometrial cancer.

Patients And Methods: Clinical features, histological type, and time to recurrence were analyzed in 640 endometrial cancer patients without residual tumours.

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