Quantitative evaluation of reversed-phase packing material based on calcium carbonate microspheres modified with an alternating copolymer.

Considering the vulnerability of silica gel to alkaline mobile phases, a highly alkaline stable stationary phase for HPLC is required to separate basic compounds with high separation efficiency. To address this issue, we have developed a high alkaline stable packing material (CaCO-PMAcO) based on mesoporous calcium carbonate microspheres modified with poly(maleic acid-alt-1-octadecene). In this study, we report further investigation of the separation performance of CaCO-PMAcO column by systematically evaluating the effects of particle size and chromatographic conditions.