Publications by authors named "Koga-Ito C"

Tracheal stenosis (i.e., the abnormal narrowing of the trachea) can occur due to a variety of inflammatory and infectious processes as well as due to therapeutic procedures undertaken by the patient.

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Oral mucositis associated with candidiasis can causes systemic candidemia, posing a risk to cancer patients administered antineoplastic therapy. Cold atmospheric pressure plasma jets (CAPPJs) have antifungal and anti-inflammatory properties. This study evaluated the effects CAPPJs in preventing systemic fungal dissemination in a murine model of oral mucositis associated with candidiasis.

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  • This study introduces an inexpensive plasma device that employs a 3D-printed design using a parallel-plate dielectric barrier discharge with a metallic mesh electrode.
  • The device operates on atmospheric air without requiring additional gas supply and significantly modifies the surface composition of polyethylene, reducing carbon levels and increasing oxygen after treatment.
  • It effectively reduces microbial load by over 99% within 1 to 10 minutes while maintaining Vero cell viability between 91% and 96%, indicating its potential for material surface modifications and infection control applications.
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This study aimed to determine the effects of low-temperature plasma jet produced in gas helium (LTP-helium) on cariogenic biofilms composedby and , and also by the combination of and . Biofilms were treated for 1, 3, 5, and 7 minutes. A 0.

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The primary aim of this study was to investigate the impact of treatment with low-temperature plasma (LTP) for varying exposure durations on a multispecies cariogenic biofilm comprising C. albicans, L. casei, and S.

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To evaluate the behavior of oral keratinocytes in the presence of Vitamin C (Vit C) and its anti-inflammatory potential. Oral keratinocytes were initially exposed to 0.1-2.

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  • Silicone endotracheal prostheses are good for medical use, but they often develop harmful biofilms that can cause inflammation and complications.
  • The study tested cold atmospheric plasma (CAP) to see if it could inhibit these harmful biofilms, and also assessed its effects on human bronchial cells.
  • Results showed that CAP significantly reduced biofilm viability without being harmful to the bronchial cells, suggesting it could be a useful method to prevent biofilm formation on silicone prostheses.
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  • This study investigates how cold atmospheric plasma (CAP) affects the structure and biochemical composition of fungal cell walls, which is not well-understood.
  • Researchers used atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) to analyze changes in cell morphology and composition after treating fungi with Helium-CAP, compared to the drug caspofungin.
  • The results showed significant changes in the fungi's surface roughness and shape, along with a decrease in glucans and an increase in chitin production, indicating that CAP alters the fungal cell wall composition.
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  • The failure of endodontic treatments is often linked to leftover microorganisms due to challenges in effectively removing biofilms, with traditional irrigation methods having limitations.
  • Non-thermal atmospheric pressure plasma (NTPP) emerges as a promising solution, with the potential for direct application on biological surfaces or through plasma-activated liquids, demonstrated in a review of 17 studies from 2007 to 2022.
  • NTPP exhibits strong antimicrobial properties, particularly with longer exposure times, and is most effective when used in combination with conventional antimicrobial solutions, although more standardized research is necessary to fully understand its application in endodontics.
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In this study, different plasma-activated liquids were evaluated for their antimicrobial effects against , as well as for their cytotoxicity on mammalian cells. The PALs were prepared from distilled (DIS), deionized (DI), filtered (FIL), and tap (TAP) water. Additionally, 0.

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  • Soft reliners and glazes are used in dental prosthetics to reduce pressure on tissues and treat conditions like dental stomatitis, sometimes incorporating medications.
  • The study focused on the antimicrobial effects of silver-coated silica nanoparticles added to these materials, leading to the creation of nanocomposites for testing.
  • Results showed that while non-functionalized nanoparticles reduced fungal growth at higher concentrations, functionalized nanoparticles performed better in tests, though improvements are needed to enhance their antifungal efficacy.
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The aim of this study was to evaluate the effect of the essential oil and its association with chlorhexidine on cariogenic microcosm biofilm composition and acidogenicity. Minimum inhibitory and bactericide concentrations from the essential oil and chlorhexidine were determined by broth microdilution assay. Microcosms (polymicrobial) biofilms were produced on glass coverslips, using inoculum from human saliva in McBain culture medium (0.

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Objective: The aim of this study was to assess the clinicopathological features of florid cemento-osseous dysplasia (FCOD)-related osteonecrosis highlighting their histopathological aspects and bone structure.

Methods: Twenty-two FCOD-related osteonecrosis cases were evaluated retrospectively. Osteonecrosis, osteomyelitis, bacterial colonization, bone resorption, reactive bone, osteon-like structure, lamellar bone, and basophilic lines were analyzed.

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The activation of water by non-thermal plasma creates a liquid with active constituents referred to as plasma-activated water (PAW). Due to its active constituents, PAW may play an important role in different fields, such as agriculture, the food industry and healthcare. Plasma liquid technology has received attention in recent years due to its versatility and good potential, mainly focused on different health care purposes.

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  • Antifungal resistance is becoming a significant issue in medicine, highlighting the need for new treatment options for fungal infections, particularly superficial candidiasis.
  • Cold atmospheric plasma (CAP) has shown promise as a treatment method, demonstrating effectiveness against biofilms in laboratory and clinical settings, but its interaction with traditional antifungals is not well understood.
  • The study found that CAP alone was more effective than traditional antifungals like nystatin and amphotericin B, and while CAP can work in combination with them, its standalone use yielded better results against biofilms.
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  • Traditional periodontal therapies have limitations, and research suggests that cold atmospheric plasma may serve as an effective adjunct treatment for periodontitis due to its anti-inflammatory and antimicrobial properties.
  • The study involved testing plasma treatments on bacterial growth in both planktonic and biofilm forms, as well as conducting in vivo experiments with mice to evaluate the effects of plasma on periodontal recovery.
  • Results indicated that plasma treatment can successfully inhibit bacterial growth without harming fibroblast viability, and appeared to enhance recovery of periodontal tissues, especially with multiple applications.
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The inhibitory and antibiofilm effects of (EOTv) and essential oils (EOHs) on cariogenic microorganisms were evaluated. The chemical characterization of EOTv was performed by gas chromatography/mass spectrometry. , , , , , and were used for agar diffusion assays and determination of minimal inhibitory and minimal bactericide concentrations.

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The increase in the prevalence of fungal infections worldwide and the rise in the occurrence of antifungal resistance suggest that new research to discover antifungal molecules is needed. The aim of this study was to evaluate the potential use of ellagic acid-cyclodextrin complexes (EA/HP-β-CD) for the treatment of oral candidiasis. First, the effect of EA/HP-β-CD on planktonic cells and biofilms was evaluated.

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Objective: This study aimed at assessing the oral prevalence ofCandida species in cystic fibrosis patients and the antifungal susceptibility of the isolates.

Design: One hundred patients aged 3-20 years old were included in the study and were divided into three groups: G1 (low severity disease): 25 cystic fibrosis patients with Shwachman-Kulczycki score (SK) between 100 and 71; G2 (high severity disease): 25 cystic fibrosis patients with SK score under 40; and G3 (control): 50 healthy patients age- and gender-matched to cystic fibrosis patients. Stimulated saliva samples were collected and the oral fungal concentrations were assessed.

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Objective: To evaluate the phenotypic features of the masticatory biomechanics in atypical subjects with Down syndrome (DS). Its influence was analysed on sleep disorders, body adiposity and its risks, and some physicochemical properties of saliva.

Methods: Seventy subjects were enrolled to assess masticatory biomechanical function and divided into two groups: DS and control groups.

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  • The study focuses on the interactions between two bacteria, Streptococcus mutans and Actinomyces naeslundii, to understand their biofilm relationships.
  • The researchers created both single and dual-species biofilms and assessed their growth, stress responses to chlorhexidine, and metabolic activities, like lactic acid production.
  • Findings indicated that dual-species biofilms showed reduced viability and lactic acid production, but had higher resistance to chlorhexidine, with unique cluster formations observed under microscopy.
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  • - The study investigated how cold atmospheric pressure plasma (CAPP) jet affects the growth, germination, and adherence of the fungus Trichophyton rubrum, which causes nail infections.
  • - Laboratory tests showed that exposing T. rubrum to plasma jet for 10 and 15 minutes completely stopped its mycelial growth and significantly reduced its ability to germinate and infect nail samples.
  • - Biochemical analysis confirmed these findings, indicating that a single 15-minute exposure to CAPP is a potentially effective treatment for fungal nail infections caused by T. rubrum.
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To investigate biopharmaceutical and antifungal properties of pure and complexed ellagic acid. Caco-2 cells cultured in a Transwell inserts were infected with to develop an model. Ellagic acid was complexed with cyclodextrins.

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Background: There are many comorbidities associated with Down syndrome (DS), including obstructive sleep apnea (OSA) and masticatory muscle alteration. Muscular hypotonia, in particular, of the masticatory and oropharyngeal muscles is one of the main characteristics of individuals with DS, resulting in impairments of speech, swallowing, and mastication in these individuals. In addition, total or partial obstruction of the airways during sleep can occur due to pharyngeal hypotonia, leading to snoring and to OSA.

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In the present study, we report the preparation of antifungal and non-cytotoxic polymer nanocomposites with potential application in biomedical materials. Dodecanethiol-protected silver nanoparticles (AgNPs-DDT) were synthesized by a reduction/precipitation method and dispersed in chloroform to obtain stable colloidal dispersions. PBAT-based nanocomposites containing 0.

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