CRIME-Q-a unifying tool for critical appraisal of methodological (technical) quality, quality of reporting and risk of bias in animal research.

Background: Systematic reviews within the field of animal research are becoming more common. However, in animal translational research, issues related to methodological quality and quality of reporting continue to arise, potentially leading to underestimation or overestimation of the effects of interventions or prevent studies from being replicated. The various tools and checklists available to ensure good-quality studies and proper reporting include both unique and/or overlapping items and/or simply lack necessary elements or are too situational to certain conditions or diseases.

Bio-integrated carbon capture and utilization: at the interface between capture chemistry and archaeal CO reduction.

Carbon capture and utilization (CCU) covers an array of technologies for valorizing carbon dioxide (CO). To date, most mature CCU technology conducted with capture agents operates against the CO gradient to desorb CO from capture agents, exhibiting high energy penalties and thermal degradation due to the requirement for thermal swings. This Perspective presents a concept of Bio-Integrated Carbon Capture and Utilization (BICCU), which utilizes methanogens for integrated release and conversion of CO captured with capture agents.

Relevance of extracellular electron uptake mechanisms for electromethanogenesis applications.

Electromethanogenesis has emerged as a biological branch of Power-to-X technologies that implements methanogenic microorganisms, as an alternative to chemical Power-to-X, to convert electrical power from renewable sources, and CO into methane. Unlike biomethanation processes where CO is converted via exogenously added hydrogen, electromethanogenesis occurs in a bioelectrochemical set-up that combines electrodes and microorganisms. Thereby, mixed, or pure methanogenic cultures catalyze the reduction of CO to methane via reducing equivalents supplied by a cathode.

Prognostic factors to predict postoperative survival in patients with recurrent glioblastoma.

Background: There are no generally accepted criteria for selecting patients with recurrent glioblastoma for surgery. This retrospective study in a Danish population-based cohort aimed to identify prognostic factors affecting postoperative survival after repeated surgery for recurrent glioblastoma and to test if the preoperative New Scale for Recurrent Glioblastoma Surgery (NSGS) developed by Park CK et al could assist in the selection of patients for repeat glioblastoma surgery.

Methods: Clinical data from 66 patients with recurrent glioblastoma and repeated surgery were analyzed.

The Selenoproteome as a Dynamic Response Mechanism to Oxidative Stress in Hydrogenotrophic Methanogenic Communities.

Methanogenesis is a critical process in the carbon cycle that is applied industrially in anaerobic digestion and biogas production. While naturally occurring in diverse environments, methanogenesis requires anaerobic and reduced conditions, although varying degrees of oxygen tolerance have been described. Microaeration is suggested as the next step to increase methane production and improve hydrolysis in digestion processes; therefore, a deeper understanding of the methanogenic response to oxygen stress is needed.

Continuous biomethanation of flue gas-carbon dioxide using bio-integrated carbon capture and utilization.

Biomethanation of carbon dioxide (CO) from flue gas is a potential enabler of the green transition, particularly when integrated with the power-to-gas chain. However, challenges arise in achieving synthetic natural gas quality when utilizing CO from diluted carbon sources, and the high costs of CO separation using amine-based solutions make large-scale implementation unfeasible. We propose an innovative continuous biomethanation system that integrates carbon capture and CO stripping through microbial utilization, eliminating expenses with the stripper.

High risk of ischaemic stroke amongst patients with hereditary haemorrhagic telangiectasia.

Background And Purpose: Hereditary haemorrhagic telangiectasia (HHT) is a genetic disease with fragile blood vessels and vascular malformations, potentially causing neurological manifestations, including stroke and cerebral abscesses. The study aimed to investigate neurological manifestations in the Danish HHT database, focusing on pulmonary arteriovenous malformations (PAVMs) as a risk factor for cerebral events.

Methods: Retrospective analysis of the Danish HHT database was conducted, cross-referencing neurological outcomes with the Danish Apoplexy Register for accuracy.

Meningioma animal models: a systematic review and meta-analysis.

Background: Animal models are widely used to study pathological processes and drug (side) effects in a controlled environment. There is a wide variety of methods available for establishing animal models depending on the research question. Commonly used methods in tumor research include xenografting cells (established/commercially available or primary patient-derived) or whole tumor pieces either orthotopically or heterotopically and the more recent genetically engineered models-each type with their own advantages and disadvantages.

Exploring increased hydraulic retention time as a cost-efficient way of valorizing residual biogas potential.

Effective substrate utilization with low residual methane yield in the digestate is crucial for the economy and sustainability of biogas plants. The composition and residual methane potential of 29 digestate samples from plants operating at hydraulic retention times of 13-130 days were determined to evaluate the economic viability of extended digestion. Considerable contents of fermentable fractions, such as cellulose (8-23%), hemicellulose (1-18%), and protein (13-22%), were present in the digestate dry matter.

Enhanced process control of trickle-bed reactors for biomethanation by vertical profiling directed by hydrogen microsensor monitoring.

Biomethanation is an emerging Power-to-X technology enabling CO valorisation to produce biomethane using renewable H. A promising reactor for facilitating biomethanation is the trickle bed reactor (TBR), however, these bioreactors are conventionally operated with a black-box approach, where the system is solely described by the input and output characteristics. This study employed a novel approach for process surveillance of internal dynamics in TBRs by installing multiple H microsensors along its vertical axis.

Reducing greenhouse gas emissions from pig slurry by acidification with organic and inorganic acids.

Methane (CH4) emission from pig slurry is a large contributor to the climate footprint of livestock production. Acidification of excreta from livestock animals with sulfuric acid, reduce CH4 emission and is practiced at many Danish farms. Possible interaction effects with other acidic agents or management practices (e.

Diagnosis, treatment, and outcome of primary CNS lymphoma-a single-center experience.

Background: Primary CNS lymphoma (PCNSL) is a highly aggressive non-Hodgkin lymphoma (NHL) that occurs in the CNS (e.g. brain, meninges, spinal cord, cerebrospinal fluid, or intraocular involvement) in the absence of systemic NHL.

Wood-Ljungdahl pathway utilisation during in situ H biomethanation.

Biogas production from organic waste is a waste-to-energy technology with the potential to contribute significantly to sustainable energy production. Upgrading of biogas using in situ biomethanation with hydrogen has the potential for surplus electricity storage, and delivery of biogas with a methane content of >90%, allowing for easier integration into the natural gas grid, as well as conversion to other products. Microbial communities in biomethanation reactors undergo changes, however, these changes are largely unexplored.

Hydrodynamic analysis of full-scale in-situ biogas upgrading in manure digesters.

The addition of hydrogen to anaerobic digesters is an emerging technique for the sustainable upgrading of biogas to biomethane with renewable electricity. However, it is critically dependent on the effective gas-liquid transfer of hydrogen, which is a sparingly soluble gas. Very little is known about the impact of liquid and gas flow and bubble size on gas-liquid transfer during H injection in full-scale anaerobic digesters.

A nuclear proteome localization screen reveals the exquisite specificity of Gpn2 in RNA polymerase biogenesis.

The GPN proteins are a conserved family of GTP-binding proteins that are involved in the assembly and subsequent import of RNA polymerase II and III. In this study, we sought to ascertain the specificity of yeast GPN2 for RNA polymerases by screening the localization of a collection of 1350 GFP-tagged nuclear proteins in WT or mutant cells. We found that the strongest mislocalization occurred for RNA polymerase II and III subunits and only a handful of other RNAPII associated proteins were altered in mutant cells.

Cellulolytic and Xylanolytic Microbial Communities Associated With Lignocellulose-Rich Wheat Straw Degradation in Anaerobic Digestion.

The enzymatic hydrolysis of lignocellulosic polymers is generally considered the rate-limiting step to methane production in anaerobic digestion of lignocellulosic biomass. The present study aimed to investigate how the hydrolytic microbial communities of three different types of anaerobic digesters adapted to lignocellulose-rich wheat straw in continuous stirred tank reactors operated for 134 days. Cellulase and xylanase activities were monitored weekly using fluorescently-labeled model substrates and the enzymatic profiles were correlated with changes in microbial community compositions based on 16S rRNA gene amplicon sequencing to identify key species involved in lignocellulose degradation.

Fluorescein-guided resection of cerebral metastases is associated with greater tumor resection.

Background: Sodium fluorescein (fluorescein) crosses a disrupted blood-brain barrier similarly to gadolinium contrast in contrast-enhancing cerebral tumors. When exposed to light with 560 nm wavelength during surgery, fluorescein emits a yellow-green fluorescent light that can be visualized through an operating microscope equipped with an appropriate emission filter. The distribution of the fluorescence correlates with the contrast on a gadolinium contrast-enhanced MRI.

The Flipped Classroom: An Innovative Approach to Medical Education in Ophthalmology.

Objective: The aim of the study is to report the creation of a flipped ophthalmology course and preclinical medical student perceptions and knowledge gains before and after a flipped ophthalmology course.

Design: The form of the study discussed is an observational study.

Subjects: The subjects involved in the study are second-year (U.

gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse.

A bacterial strain, designated WCA-9-b2, was isolated from the caecal content of an 18-week-old obese C57BL/6NTac male mouse. According to phenotypic analyses, the isolate was rod-shaped, strictly anaerobic, spore-forming, non-motile and Gram-stain-positive, under the conditions tested. Colonies were irregular and non-pigmented.

Stick or leave - Pushing methanogens to biofilm formation for ex situ biomethanation.

Biomethanation exploits the ability of methanogenic archaea to convert CO and renewable H from electrolysis to biomethane. Biofilm reactors are promising for biomethanation scale-up due to high CH productivity and low energy input for H gas-liquid mass transfer. Effects of operational conditions on biofilm dynamics remain largely uncharacterized but may increase reactor potentials further.

Patients', Pharmacy Staff Members', and Pharmacy Researchers' Perceptions of Central Elements in Prescription Encounters at the Pharmacy Counter.

: Studies suggest that the way pharmacy counselling takes place does not fully support patients in obtaining optimal medicine use. To understand the basis of current challenges in pharmacy counselling, we investigated which selected related cues, i.e.

Biogas upgrading with hydrogenotrophic methanogenic biofilms.

Hydrogen produced from periodic excess of electrical energy may be added to biogas reactors where it is converted to CH that can be utilized in the existing energy grid. The major challenge with this technology is gas-to-liquid mass transfer limitation. The microbial conversions in reactors designed for hydrogenotrophic methanogenesis were studied with microsensors for H, pH, and CO.

[Job] Locked and [Un]loaded: The effect of the Affordable Care Act dependency mandate on reenlistment in the U.S. Army.

One concern with employer-based health insurance is job lock or the inability for employees to leave their current employment for better opportunities for fear of losing benefits. We use the implementation of the Affordable Care Act's dependency mandate as a natural experiment. Data from the United States Army overcome some limitations in previous studies including the ability to examine workers with fixed contract expiration dates, uniform pay, and health coverage.

How Low Can You Go: Methane Production of at Low CO Concentrations.

Autotrophic hydrogenotrophic methanogens use H/CO as sole carbon and energy source. In contrast to H, CO is present in high concentrations in environments dominated by methanogens e.g.