Impact of low and high temperatures on aerobic granular sludge treatment of industrial wastewater.

The goal of this study was to unravel the impact of high and low temperatures (T) on glycogen-accumulating microorganisms (GAOs) which were stimulated in an aerobic granular sludge plant fed with industrial wastewater, which is derived from the cleaning of trucks transporting chocolate and beer. Among GAOs, Candidatus Competibacter (Ca. Competibacter) was the most abundant.

Performance of an aerobic granular sludge membrane filtration in a full-scale industrial plant.

This study quantifies the hydraulic performance of a pilot-scale ultrafiltration system integrated into a full-scale industrial aerobic granular sludge (AGS) plant. The treatment plant consisted of parallel AGS reactors, Bio1 and Bio2, with similar initial granular sludge properties. During the 3-month filtration test, a chemical oxygen demand (COD) overloading episode took place, affecting the settling properties, morphology, and microbial community composition in both reactors.

Is building up substrate during anaerobic feeding necessary for granulation?

For a successful granulation process in activated sludge systems, the stimulation of slow growing organisms such as glycogen accumulating microorganisms (GAOs) is a key factor. Here we show that the introduction of an anaerobic feast followed by an aerobic famine phase successfully transforms bulking sludge, caused by the abundance of genus Kouleothrix, to a hybrid floccular-granular sludge. Two sequencing batch reactors (SBRs) were operated for 228 days treating the same industrial wastewater derived from the cleaning of trucks transporting liquid food (the cargo consists of approximately 70% chocolate and 30% beer).

Pressure- and temperature-induced unfolding and aggregation of recombinant human interferon-gamma: a Fourier transform infrared spectroscopy study.

The effect of hydrostatic pressure on the secondary structure of recombinant human interferon-gamma (rhIFN-gamma) and its biologically inactive truncated form rhIFN-Delta C15 has been studied using Fourier-transform IR (FTIR) spectroscopy. In situ observation of the pressure-induced changes using the diamond anvil cell shows that the alpha-helical structure is mainly transformed into disordered structure at high pressure. Increasing pressure also induces the formation of a gel.