Publications by authors named "Koel Sen Baksi"
Article Synopsis
- The study explored the use of circulating tumor DNA (ctDNA) as a "liquid biopsy" for children with solid tumors, focusing on its feasibility and clinical usefulness in real-time monitoring of their conditions.
- Out of 240 patients, plasma samples from 217 were analyzed, achieving a high success rate of 99.5% for extracting and quantifying cell-free DNA.
- The ctDNA analysis showed better mutation detection rates in non-CNS solid tumors and metastatic cases, suggesting its potential for tracking cancer progression in young patients.
Background: Pediatric papillary thyroid carcinoma (PTC) is clinically and biologically distinct from adult PTC. We sequenced a cohort of clinically annotated pediatric PTC cases enriched for high-risk tumors to identify genetic alterations of relevance for diagnosis and therapy.
Methods: Tumor DNA and RNA were extracted from FFPE tissue and subjected to next-generation sequencing (NGS) library preparation using a custom 124-gene hybridization capture panel and the 75-gene Archer Oncology Research Panel, respectively.