Impact of familiarity with the format of the exam on performance in the OSCE of undergraduate medical students - an interventional study.

Background: Assessments, such as summative structured examinations, aim to verify whether students have acquired the necessary competencies. It is important to familiarize students with the examination format prior to the assessment to ensure that true competency is measured. However, it is unclear whether students can demonstrate their true potential or possibly perform less effectively due to the unfamiliar examination format.

3D-cultivation of NSCLC cell lines induce gene expression alterations of key cancer-associated pathways and mimic conditions.

This work evaluated gene expression differences between a hanging-drop 3D NSCLC model and 2D cell cultures and their relevance by comparison to patient-derived data from The Cancer Genome Atlas. Gene expression of 2D and 3D cultures for Colo699 and A549 were assessed using Affymetrix HuGene 1.0 ST gene chips.

The influence of stromal cells and tumor-microenvironment-derived cytokines and chemokines on CD3CD8 tumor infiltrating lymphocyte subpopulations.

The tumor microenvironment has been identified as a major mediator of immunological processes in solid tumors. In particular, tumor-associated fibroblasts are known to interact with tumor infiltrating immune cells. We describe the influence of fibroblasts and tumor-microenvironment-derived cytokines on the infiltration capacity of CD3CD8 cytotoxic T lymphocyte subpopulations using a multicellular 3D co-culture system.

Development of a 3D angiogenesis model to study tumour - endothelial cell interactions and the effects of anti-angiogenic drugs.

The tumour microenvironment and tumour angiogenesis play a critical role in the development and therapy of many cancers, but in vitro models reflecting these circumstances are rare. In this study, we describe the development of a novel tri-culture model, using non-small cell lung cancer (NSCLC) cell lines (A549 and Colo699) in combination with a fibroblast cell line (SV 80) and two different endothelial cell lines in a hanging drop technology. Endothelial cells aggregated either in small colonies in Colo699 containing microtissues or in tube like structures mainly in the stromal compartment of microtissues containing A549.

Evaluation of assays for drug efficacy in a three-dimensional model of the lung.

Background: The focus of the outlined work is the establishment of a three-dimensional lung model for various drug-screening applications.

Methods: The non-small cell lung cancer (NSCLC) cell line Colo699 was cultivated as monolayer (2D) on plates for 5 days or as microtissues (3D) using a hanging-drop system for 5 and 10 days. Cells and microtissues were treated with afatinib (10-80 µM), cisplatin (100-800 µM) or vinorelbine (25-200 µM) for 24 or 48 hours (h).

The impact of metformin and salinomycin on transforming growth factor β-induced epithelial-to-mesenchymal transition in non-small cell lung cancer cell lines.

The epithelial-to-mesenchymal transition (EMT) is highly involved in the development of metastases. EMT transforms epithelial carcinoma cells into mesenchymal-like cells, characterized by increased cell migration and invasiveness. Transforming growth factor β (TGFβ) appears to be crucial in this process.

Infiltration of lymphocyte subpopulations into cancer microtissues as a tool for the exploration of immunomodulatory agents and biomarkers.

Introduction: The interaction between the immune system and malignant diseases is a proven key target for cancer therapy. We describe an innovative 3D cell culture system comprising both immune and cancer cells to evaluate their interaction and immune cell infiltration to provide an innovative in vitro screening of immunomodulatory agents and biomarkers.

Methods: 3D tumor microtissues were cultivated using a hanging drops system.

Development of an innovative 3D cell culture system to study tumour--stroma interactions in non-small cell lung cancer cells.

Introduction: We describe a novel 3D co-culture model using non-small cell lung cancer (NSCLC) cell lines in combination with lung fibroblasts. This model allows the investigation of tumour-stroma interactions and addresses the importance of having a more in vivo like cell culture model.

Methods: Automation-compatible multi-well hanging drop microtiter plates were used for the production of 3D mono- and co-cultures.

Patients with cancer and family caregivers: management of symptoms caused by cancer or cancer therapy at home.

People are diagnosed with cancer sooner nowadays thanks to increased awareness and improvements in cancer screenings. Patients are able to live longer due to cancer treatment regimens; however, they suffer the consequences of living with cancer and therapy-related symptoms. Symptom management is challenging for both patients and family caregivers.

[Mentally disturbed offenders in Flanders].

In the course of the past 25 years in Flanders there has been a real breakthrough as far as forensic psychiatry is concerned. New types of treatment have become available for mentally disturbed offenders, whether they are in prison or in the community. A new Belgian law on mentally disturbed offenders with diminished responsibility confirms that 'internment' is still regarded as a protective measure and an alternative to punishment.