[Characteristics of the effect of implantation of the human ApoA1 gene on the condition of hepatocytes in rats of varying age].

The results obtained show the essential changes in functional state of hepatocyte's plasmatic membrane due to the implantation of human ApoA1 gene to the rat liver. The changes in phospholipid composition, hyperpolarization, increase in activity of membrane bound enzymes, cytochrome P-450 and biosynthesis of liver total proteins have been found. The essential changes characterizing cell effect were more marked in the adult rats, and membrane effect in the old ones.

[Effect of partial hepatectomy on the invertor mechanism of regulation of the Na+,K+-ATPase activity in hepatocytes of rats of various ages].

Age peculiarities of partial hepatectomy effect on the hepatocytes plasma membrane Na+, K(+)-ATPase activity and its insulin-induced stimulation has been studied. It has been shown that partial hepatectomy does not change basal Na+, K(+)-ATPase activity in adult rats. In old partial hepatectomised rats Na+, K(+)-ATPase activity is slightly higher than in control old rats, although this increase is not statistically significant.

Na,K-ATPase and Ca-ATPase activities of the myocardial sarcolemma in aging rats after aorta coarctation: role of invertors.

In this work we used aorta coarctation as a model of myocardial hypertrophy. We studied the role of intracellular regulators of plasma membrane status-invertors-in the mechanisms of changes of membrane enzyme activities in the emergency stage of myocardial hypertrophy. We used Wistar rats of various ages: adult (6-8 months) and old (26-28 months) rats.

Effects of partial hepatectomy on the plasma membrane status and the invertor mechanism of the hepatocyte Na,K-ATPase activity regulation in rats of various age.

The experiments were performed on adult (6-8 months) and old (22-24 months) Wistar rats. Insulin induced plasma membrane hyperpolarization and hepatocyte Na,K-ATPase activation in adult but not in old sham-operated rats. Partial hepatectomy had no effect on the invertor mechanism of Na,K-ATPase activity regulation in the liver of adult rats, while pronounced changes took place in old animals 4 weeks after partial hepatectomy.

[The effect of the implantation of the human apo A-I gene on the function of the hepatocyte plasma membrane in rats].

Evidences are given that implantation of human apo A-I gene to experimental animals induces essential changes in the functional state of hepatocyte plasmatic phospholipid composition, hyperpolarization development, as well as changes in the Na, K-ATPase and adenylate cyclase activities. The presence of generalized cell reaction is evidenced from the fact than biosynthesis of summary proteins gets enhanced during the above changes.

Changes in the plasmatic membrane characteristics during microsomal monooxygenase induction in the liver of adult and old rats.

The experiments on adult (6-8 months) and old (24-26 months) male Wistar rats have shown that treatment of animals with phenobarbital results in a significant increase in hepatic microsomal enzyme content, plasmatic membrane Na+, K(+)-ATPase activities and the elevation of hepatocyte membrane potential value. It is presumed that the changes in plasmatic membrane characteristics during microsomal monooxygenase induction are related to the synthesis of specific intracellular factors (invertors). This assumption was verified by the experiments with 'cellular hybrid' system (cytosol--plasmatic membranes).

[The morphological evaluation of a heart ventricle made from the latissimus dorsi muscle in an experiment].

In experiment of 16 animals the morpho-functional transformations taking place in skeletal-muscular transplant while various methods of artificial heart ventricle creation and his training application were studied. It was established that, as a rule, in autotransplant the reversible structural changes are taking place, what stipulates the necessity of keeping of the "latent" period 14-21 days long between the operation conducted and the beginning of training stimulation of heart ventricles, manufactured of a skeletal muscle.

[The content of free amino acids and biogenic amines in the urine of hypertension patients].

Contents of free amino acids and some biogenic amines were studied in hypertonic patients at the age of 40-50 years. The study demonstrates quantitative changes in levels of free amino acids and some biogenic amines caused by disorders of protein metabolism. The data obtained may be used for diagnosis of hypertonic diseases.

Plasmic membranes of hepatocytes and adrenocorticocytes in rats of different ages: effect of testosterone.

Experiments on adult (6-8-month-old and 26-28-month-old) Wistar rats revealed the hyperpolarization of plasmic membranes and activation of Na,K-ATPase of adrenocorticocytes in animals of both age groups and of hepatocytes of adult rats. No effect of testosterone was observed on the level of membrane potential and the activity of Na,K-ATPase of hepatocytes of old rats. The effect of testosterone was prevented by inhibitors of protein biosynthesis (actinomycin D and cycloheximide) and a specific inhibitor of Na,K-ATPase (ouabain), but not by K(+)-channel blocker 2-aminopyridine.

[Membrane mechanisms of testosterone action].

The experiments on adult Wistar rats have shown that testosterone administration provoke hyperpolarization of hepatocytes and adrenocorticocytes plasmatic membranes. It was discovered that this hyperpolarization was caused by cell Na, K-ATPase activation. Inhibitors of protein biosynthesis prevent both testosterone-induced hyperpolarization and Na, K-ATPase activation.

[The fatty acid test in patients with the initial stages of hypertension].

It was established that initial stages of hypertensive disease are accompanied by quantitative changes of the content of free fatty acids. An important diagnostic criterion in the pathogenesis of this disease are unsaturated fatty acids. Their concentration was studied in erythrocytes using gaseous-fluid chromatography.

[Polyunsaturated fatty acids in the diagnosis of cardiovascular diseases].

Blood plasma and red cell membrane levels of polyunsaturated fatty acids were measured by gas chromatography in patients with Stage I essential hypertension, borderline arterial hypertension, functional classes II-IV unstable angina pectoris, and unstable angina. The findings suggest that insufficient linolenic acid level in blood plasma and red cell membranes and, in the majority of cases, elevated concentrations of linoleic acid are among lipid metabolism disorders in cardiovascular patients. This fact permits a conclusion that measurements of blood plasma and red cell membrane levels of polyunsaturated fatty acids may be an additional diagnostic criterion permitting an assessment of the disease severity and elucidation of the pathogenesis of coronary disease and arterial hypertension.

[Blood vessels and nerve apparatus of the heart and small intestine during continuous experimental bypass shunting].

In 140 animals (mongrel dogs of different age and sex) by means of histological, neurohistological, histochemical, histoenzymatic and electron microscopic methods, character and dynamics in changes of nerve elements and blood vessels of the heart and the small intestine have been studied under the conditions of revascularization at aorto-coronary and aorto-mesenteric shunting. The changes revealed are mainly reactive in their character, perform defensive, compensatory-adaptive response of the organs to the orative trauma and to an adequate but slightly changed blood stream. The degree of acuteness and the time when these changes disappear depends on duration of postocclusive ischemia, on the organs' resistivity to its effect on the operative trauma.