Publications by authors named "Ko-ichi Hamano"
Relaxin and insulin-like growth factor (IGF)-I have pronounced effects on the male and female reproductive tracts. The aim of this study was to investigate the effects of relaxin and IGF-I on the motility, capacitation, acrosome reaction, cholesterol efflux and utilization of glucose in porcine spermatozoa. Swim-up separated spermatozoa that had been washed twice were incubated at 37°C for 1 or 4 h in modified Tyrode's albumin lactate pyruvate (mTALP) medium supplemented without (control) or with relaxin (20 ng/mL) or IGF-I (20 ng/mL) or both (10 + 10 ng/mL).
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- Relaxin is a peptide hormone that significantly enhances sperm motility and stimulates the acrosome reaction in boar spermatozoa when incubated with specific concentrations.
- The study found that treatment with relaxin (20 or 40 ng/ml) led to higher percentages of acrosome-reacted live sperm and improved glucose metabolism, as indicated by increased incorporation and oxidation rates of labeled glucose.
- Overall, relaxin positively affects sperm function, contributing to both motility and energy utilization during incubation.
Porcine relaxin is a peptide hormone belonging to the insulin super family that has a variety of biological functions. The present experiment was designed to investigate the effects of relaxin on sperm function and on in vitro fertilization (IVF) of porcine oocytes. Porcine spermatozoa were washed, swum-up, and incubated for 1-4 h in mTALP medium supplemented with 0, 20 or 50 ng/ml porcine relaxin.
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