Development of an Ex Vivo Porcine Eye Model for Exploring the Pathogenicity of .

, a widely distributed free-living amoeba found in various environments, is an opportunistic pathogen responsible for causing keratitis, a condition that may lead to blindness. However, identifying the pathogenicity of is challenging due to its complex life cycle, ability to adapt to different environments, variable virulence factors, and intricate interactions with the host immune system. Additionally, the development of an effective model for studying pathogenicity is limited, hindering a comprehensive understanding of the mechanisms underlying its virulence and host interactions.

Enhancement of capsular hypermucoviscosity in Klebsiella pneumoniae by Acanthamoeba.

Background: Acanthamoeba and Klebsiella pneumoniae are both environmental commensals. Recently, clinical harm caused by hypermucoviscous K. pneumoniae has been observed.

Comparison of Microbial Sampling Sites and Donor-Related Factors on Corneal Graft Contamination.

Grafts used for corneal donation should be sterile to avoid transplantation failure and secondary infection. However, there are no clear and globally accepted specifications from eye banks on microbial sampling sites. The objective of this study was to analyze microbial contamination of corneal grafts collected from different sampling sites.