Channels at the catalytic site of glycogen phosphorylase b: binding and kinetic studies with the beta-glycosidase inhibitor D-gluconohydroximo-1,5-lactone N-phenylurethane.

Regions of low packing density in the vicinity of the catalytic site of glycogen phosphorylase b are described with the aid of a computer program that generates a contour map in which the contour level is inversely proportional to the packing density in the protein. It is shown that, although there is no direct route from the catalytic site to the surface, there are two possible channels that could allow access for substrates following conformational changes in the enzyme. The first channel, channel 1, leads from the catalytic site to the surface close to the nucleoside inhibitor site and requires movements of residues 280-285 and Arg 569 in order to obtain access.

The isolation and characterisation of human atrial natriuretic factor produced as a fusion protein in Escherichia coli.

Human atrial natriuretic factor [ANF(1-28)] has been isolated from a fusion protein produced in Escherichia coli. ANF(1-28) was linked to a naturally occurring E. coli protein, chloramphenicol acetyltransferase, via unique cleavage sequences susceptible to either human thrombin digestion, or the chemical action of 2-(2-nitrophenylsulphenyl)-3-methyl-3'-bromoindolenine (BNPS-skatole).

Changes in heart rate following eyes-closed alpha enhancement.

Subjects (n = 20 females 18 to 26 yr. old) who had been trained in an eyes-closed alpha-enhancement task were grouped according to performance and changes in heart rate were compared. Subjects who showed a facility for producing alpha also evidenced a small decrease in heart rate while a small increase in heart rate while a small increase in heart rate was shown by the low alpha-output group.

Effects of true and inverted feedback on integrated occipital alpha.

Ambient illumination had no effect on baseline levels of alpha of 20 female university students who had their eyes closed, and significant increases from these levels were obtained through true but not inverted feedback procedures. These results indicate that alpha enhancement can be obtained in a manner that is not explicable in terms of disinhibition processes.

Resting levels of alpha and the Eysenck Personality Inventory.

The number of microvolt seconds of alpha rhythm emitted during a five-minute period were collected for 30 male and 30 female subjects. This measure of alpha abundance was then compared in subjects who scored high and in those who scored low on the E and N scales of the Eysenck Personality Inventory. Subjects who scored low on the E scale tended to have a significantly greater number of microvolt seconds of alpha than did subjects who scored high on the scale.

Focal positive spikes in electroencephalography.

Focal positive spikes and sharp waves in clinical EEG are uncommon (about 1.3 per 1,000 EEGs in this series) and encountered chiefly in early life. All of out seven patients suffered from a convulsive disorder.

Heart rate, muscle tension, and alpha production of transcendental meditators and relaxation controls.

A group of transcendental meditators, and a group of control subjects instructed to relax, were compared with respect to degree of relaxation reached as measured by changes in heart rate, tension of the frontalis muscle, and occipital alpha production. The only significant changes were decreases in these measures over time in the control subjects. The changes in the controls were probably the consequence of sleep or the onset of sleep.

A comparison of EMG feedback and progressive muscle relaxation training in anxiety neurosis.

Two similar groups of adult psychiatric patients carrying the diagnosis of anxiety neurosis were compared in their response to different methods of training in deep muscle relaxation. One group received EMG feedback and the other a modification of the Jacobson Progressive Relaxation method. The frontalis muscle was chosen as the target for feedback training and for the measurement of tension reduction in both groups, for the reason that this muscle has been shown to reflect the general muscle tension level in anxious patients.

Subjective aspects of alpha enhancement.

Early reports on enhancing occipital alpha through feedback noted that subjects reported the experience as being pleasant and relaxing. This paper reports the subjective experiences of 140 subjects who participated in four studies which examined the alpha enhancement phenomenon. Under both eyes-open and eyes-closed conditions, approximately 50 per cent of the subjects reported that alpha enhancement was "pleasant' and 50 per cent "unpleasant/neutral'.