Performance of the Elecsys Rubella IgG assay in the diagnostic laboratory setting for assessment of immune status.

Rubella in early pregnancy bears a high risk for congenital defects (e.g., cataracts, hearing loss, and heart disease) and for long-term sequelae in the newborn.

Cytomegalovirus (CMV) seroprevalence in pregnant women, bone marrow donors and adolescents in Germany, 1996-2010.

In Germany, studies on the IgG seroprevalence in pregnancy and in women of childbearing age are rare. Therefore, we retrospectively evaluated the CMV IgG seropositive rate in 40,324 pregnant women as well as in 31,093 female and male bone marrow donors over 15 consecutive years (1996-2010). Furthermore, the result of a study conducted in 1999 investigating 1,305 healthy adolescents with known ethnicity was included.

Evaluation of a commercial IgG/IgM Western blot assay for early postnatal diagnosis of congenital toxoplasmosis.

The aim of this study was to evaluate a commercial Western blot IgG/IgM assay for use in the early serological diagnosis of congenital toxoplasmosis. This assay compares the immunological profile of mother and infant and allows differentiation between passive transmitted maternal antibodies and newly synthesized antibodies of the infant within the first 3 months of life. Over a 6-year period (1995-2001), the sera from 169 mothers and their 175 offspring (6 had twins) were examined for specific anti- Toxoplasma gondii IgG, IgM and IgA antibodies with an enzyme-linked immunosorbent assay or an immunosorbent agglutination assay.

Seroprevalence study of herpes simplex virus type 2 among pregnant women in Germany using a type-specific enzyme immunoassay.

In a German seroepidemiological study to determine the proportion of pregnant women infected with herpes simplex virus type 2 (HSV-2) and at risk of transmitting the infection to the newborn during delivery, IgG antibodies to HSV-2 in 1999 sera collected from pregnant women in 1996-1997 were measured using an automated type-specific enzyme immunoassay (Cobas Core HSV-2 IgG EIA; Roche Diagnostics, Switzerland). The seroprevalence of HSV-2 was 8.9%, and control studies with a type-common HSV assay measuring antibodies to HSV-1 and HSV-2 revealed that 20.

Rubella IgG total antibody avidity and IgG subclass-specific antibody avidity assay and their role in the differentiation between primary rubella and rubella reinfection.

The total rubella IgG antibody avidity ELISA, as described by Hedmann for differentiation between acute primary rubella and reinfection, persisting or non-specific IgM antibodies were evaluated in 212 sera of four categories of patients with known history (n = 149) and in one group (n = 39) of patients with a history of unclear nature. The development of the IgG ratio (avidity) from low (less than 30%) to higher levels (greater than 30%) was determined in 146 sera from 96 cases with acute primary rubella between days 1 to 256 after onset of exanthem, with a borderline between low (less than 30%) and high (greater than 30%) avidity after four weeks after onset of exanthem. From seven days to 14 years following vaccination, the IgG ratio increased more slowly following vaccination, with a useful borderline at 2 1/2 months.

Detection of IgM antibodies against rubella virus: comparison of two indirect ELISAs and an anti-IgM capture immunoassay.

A commercial antibody capture enzyme immunoassay (Rubenz M) was compared to two commercial indirect enzyme immunoassays (Enzygnost IgM, Rubazyme-M) for the detection of rubella-specific IgM. Five hundred and fifty-two sera collected between the day of onset and 272 days after the onset of the exanthem of primary rubella were tested. Rubenz M was more sensitive early and late after the onset of the exanthem than the two indirect ELISAs.

Comparison of various serological methods and diagnostic kits for the detection of acute, recent, and previous rubella infection, vaccination, and congenital infections.

The antibody development after natural rubella infection and rubella vaccination has been followed in 802 sera from 493 patients and 71 sera from 22 vaccinees. Also examined were 67 sera from 28 infants with rubella embryopathy and sera from 50 children with presumed prenatal infection. In addition, 777 sera from 641 patients tested for routine rubella diagnosis were studied.

Comparison of the performance and reproducibility of various serological methods and diagnostic kits for the detection of rubella antibodies.

For rubella antibody detection performance and reproducibility of standard laboratory methods and newer assay systems have been evaluated. IgM antibody detection in sucrose density gradient ultracentrifugation (SDG) was compared with two commercial ELISAs and the non-commercial anti-my-hemadsorption immunosorbent technique. The two ELISAs proved to be more sensitive than the SDG even if done with a long incubation hemagglutination inhibition test (HAI) to increase sensitivity.