An Unusual Case of Pinna Squamous Cell Carcinoma Arising Shortly after Resection of Atypical Fibroxanthoma.

Objective: To report a unique case of pinna squamous cell carcinoma (SCC) arising shortly after resection of atypical fibroxanthoma (AFX) at the same site.

Case Report: An 81-year-old gentleman presented with a nodular right pinna lesion. This was excised, and histology confirmed AFX.

Airway management in patients with subglottic stenosis: experience at an academic institution.

We describe a pilot study investigating the airway techniques used in the anesthetic management of subglottic stenosis. We searched the electronic clinical information database of the University of Michigan Health System for cases of subglottic stenosis in patients undergoing surgery. Demographics, airway techniques, incidence of hypoxemia, and technique failure were extracted from 159 records.

T-loop phosphorylation stabilizes the CDK7-cyclin H-MAT1 complex in vivo and regulates its CTD kinase activity.

Cyclin-dependent kinase (CDK)7-cyclin H, the CDK-activating kinase (CAK) and TFIIH-associated kinase in metazoans can be activated in vitro through T-loop phosphorylation or binding to the RING finger protein MAT1. Although the two mechanisms can operate independently, we show that in a physiological setting, MAT1 binding and T-loop phosphorylation cooperate to stabilize the CAK complex of Drosophila. CDK7 forms a stable complex with cyclin H and MAT1 in vivo only when phosphorylated on either one of two residues (Ser164 or Thr170) in its T-loop.

Reciprocal activation by cyclin-dependent kinases 2 and 7 is directed by substrate specificity determinants outside the T loop.

Cyclin-dependent kinase 7 (CDK7) is the catalytic subunit of the metazoan CDK-activating kinase (CAK), which activates CDKs, such as CDC2 and CDK2, through phosphorylation of a conserved threonine residue in the T loop. Full activation of CDK7 requires association with a positive regulatory subunit, cyclin H, and phosphorylation of a conserved threonine residue at position 170 in its own T loop. We show that threonine-170 of CDK7 is phosphorylated in vitro by its targets, CDC2 and CDK2, which also phosphorylate serine-164 in the CDK7 T loop, a site that perfectly matches their consensus phosphorylation site.

Inhibition of transcription by the trimeric cyclin-dependent kinase 7 complex.

Cyclin-dependent kinase 7 (CDK7) can be isolated as a subunit of a trimeric kinase complex functional in activation of the mitotic promoting factor. In this study, we demonstrate that the trimeric cdk-activating kinase (CAK) acts as a transcriptional repressor of class II promoters and show that repression results from CAK impeding the entry of RNA polymerase II and basal transcription factor IIF into a competent preinitiation complex. This repression is independent of CDK7 kinase activity.

Cloning of an M. tuberculosis DNA fragment associated with entry and survival inside cells.

Mycobacterium tuberculosis infects one-third of the world's human population. This widespread infection depends on the organism's ability to escape host defenses by gaining entry and surviving inside the macrophage. DNA sequences of M.

A Review of reference group data from normal children for the halstead-reitan neuropsychological test battery for older children.

This article reviews data gathered from samples of normal children who were assessed with the Halstead-Reitan Neuropsychological Test Battery for Older Children (HRB-OC), ages 9-14 years. Graphic comparisons by age group indicate clear developmental trends for all measures except TPT-memory, TPT-localization, and Seashore Rhythm Test. Methods used to analyze variability of the measures suggested that the following may be unreliable: Tactual Performance Test, all timed measures, memory and localization tasks; Trail Making Test, Part B among younger children; Speech-Sounds Perception Test; and Seashore Rhythm Test.

The effects of head injury in children on neuropsychological and behavioural functioning.

In a prospective study 76 children were divided into three groups on the basis of severity of head injury as defined by the Glasgow Coma Scale and duration of increased intracranial pressure. The children were administered a neuropsychological test battery and behavioural ratings were made by parents and teachers at three intervals: time of hospital discharge and 3 and 9 months post-initial testing. There were cognitive deficits related to severity of injury with the greatest difference in abilities observed between the severe and the other two groups.

Speeded performance following head injury in children.

Fifty-one children who has sustained head injuries were divided into mildly, moderately and severely injured groups according to neurological criteria. The groups were matched for age, sex, and injury-test interval. Approximately 1 year after their injuries, patients were tested on speeded and nonspeeded measures of motor, visual-motor, and visual-spatial functioning as well as on the WISC-R.

Neuropsychological deficits following head injury in children.

Children who had sustained head injuries were divided into three groups on the basis of severity of injury defined according to neurological criteria, and their performance on a battery of neuropsychological tests was compared. The groups were matched for age and sex. Performance IQ and timed tests of motor speed, fine-motor coordination, tactual-spatial functions, and verbal fluency showed significantly greater deficits for severely injured patients than for those who were mildly or moderately injured.

Chronic cerebellar stimulation in cerebral palsy.

This report summarizes the experience with chronic cerebellar stimulation in cerebral palsy patients at the Children's Hospital of Eastern Ontario. From July, 1977, to September, 1978, 12 patients suffering from cerebral palsy underwent cerebellar implant for chronic cerebellar stimulation. Postoperative evaluation was made approximately 1 week, 3 months, 6 months, and 12 months after surgery by a group of specialists using speech, respiration, muscle tone, involuntary movements, salivation, and activities of daily living as parameters, and these findings were compared with the findings prior to surgery.

Changing patterns of soft drug use prior to and during pregnancy: a prospective study.

Two hundred and seventeen women were interviewed during various stages of pregnancy to determine the extent and changing patterns of alcohol, nicotine and marihuana use in the year before pregnancy and during each trimester of pregnancy. Nutritional intake did not vary among women in the various drug using categories. Before pregnancy, 18% of the women were heavy social drinkers.

Disulfide bond-modified trypsinogen. Role of disulfide 179-203 on the specificity characteristics of bovine trypsin toward synthetic substrates.

Disulfide 179-203 of trypsinogen was cleaved and the free sulfhydryls were modified by S-carboxymethylation, S-carboxyamidomethylation, or by S-aminoethylation. The enzymatic properties of the activated, modified zymogens toward specific and nonspecific trypsin substrates were studies. The three S-alkylated trypsins hydrolyzed N-benzoylarginine ethyl ester and N-tosyl-lysine methyl ester with Kcat values similar to those of trypsin but with Km values that were increased by 2 to 3 orders of magnitude.

Effects of pemoline on hyperactive boys.

Thirty boys rated as hyperactive by their teachers and parents received penoline or placebo in a double blind design. A variety of measures were taken during the nine week blind period. Behavior ratings were made on the Conners scale at 4 time intervals showing that a significant improvement in the drug group was observed by the teachers, but no significant change occurred in the ratings by the parents and physician.