Publications by authors named "Kniazeva O"

Current 'Atlantification' of the Arctic Ocean affects benthic communities leading to the changes in their structure and abundance. Such areas as Svalbard that are seasonally affected by Atlantic and Arctic water masses may give a possibility to preliminary estimate the response of benthic communities to short-term environmental changes and to evaluate their sensitivity. We have sampled Kongsfjorden for modern benthic foraminifera in three different seasons.

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Glaciers in the fjords of Svalbard have been receding over last decades. Tempelfjorden, a typical glaciated fjord in West Spitsbergen (78°24'06″ N, 17°02'30″ E), has been sampled in summer 1995 and 2001-2007 for modern benthic foraminifera. We have normalized the abundances and unified the taxonomy of all these published and unpublished data sets and then compiled the record of foraminiferal assemblages changing over years into a comprehensive database.

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The basic principles of the design of direct multiparticle models and the results of multiparticle computer simulation of electron transfer by mobile protein carriers in the photosynthetic membrane of a chloroplast thylakoid are presented. The reactions of complex formation of the protein plastocyanin with the protein cytochrome f and the pigment-protein complex of photosystem I, as well as of the protein ferredoxin with the protein FNR and photosystem 1 are considered. The role of diffusion and electrostatic interactions is discussed, and the effect of the shape of the reaction volume and ionic strength on the rate of electron transport are discussed.

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A multiparticle computer model of plastocyanin-cytochrome f complex formation in the thylakoid lumen has been designed, which takes electrostatic interactions of proteins and the thylakoid membrane into account. The Poisson-Boltzmann formalism was used to determine the electrostatic potential field generated by the electrical charges of the proteins and the thylakoid membrane for different ionic strength values. The role of electrostatic field of the thylakoid membrane in plastocyanin-cytochrome f complex formation was determined.

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Correlation was investigated between blood-plasma levels of C3(H2)O (conformation pattern of C3 component of the complement) and tumor-associated marker CA-125 in patients with ovarian cancer before and after chemotherapy. Since a drop in CA-125 level after chemotherapy was associated with similar changes in C3(H2)O fraction, it seems reasonable to suggest that change in conformation of C3 is a response of the immune system to cancer.

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Storage-related differences in C3 level of blood complement and its hydrolized form--C3(H2O)--were identified in Hodgkin's disease patients and healthy donors, by immunoenzymatic analysis using murine monoclonal antibodies. Both C3 and C3(H2O) levels in blood serum of patients varied with time and were significantly different from those in health subjects; they correlated with EDTA concentration. After a second thawing of plasma in patients, there were no traces left of C3(H2O).

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Mouse monoclonal IgG1-antibodies (MA) against melittin were used for the investigation of human IgG-C1q binding. The ELISA method, allowing one to determine the dissociation constants of IgG-C1q complex, based on the competition of two IgG (investigating human IgG and MA, labelled by peroxidase) for C1q binding was developed. The method was used to show that IgG in 50% of cases of chronic lymphoid leukemia possessed lesser complement-binding activity than normal human IgG.

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A total of 878 children from the regions with unfavourable radiation situation were investigated in varying periods after the catastrophe. The data obtained were compared with the results of the study of 317 children from "pure" regions. Morphological, metabolic and ultrastructural changes were detected in blood cells of the children from radioactively contaminated regions after the Chernobyl catastrophe.

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