[Composite biodegradable materials based on polyhydroxyalkanoate].

Conditions for the processing and mixing of biodegradable polymers at temperatures less than their thermal destruction (130-150 degrees C) using standard equipment have been identified. The structure of the polyhydroxybutyrate/valerate (PHB/V) copolymer has been revealed and peculiarities of the crystal phase formation at different monomer ratios have been investigated. It was shown that pure PHB with molecular mass 180-270 kDa has elastic module approximately 1.

[Gene expression profiles of protein kinases and phosphatases obtained by hybridization with cDNA arrays: molecular portrait of human prostate carcinoma].

Hybridization with cDNA arrays was used to obtain expression profiles of 263 protein-tyrosine kinase (PTK), protein-tyrosine phosphatase (PTP), dual-specific phosphatase (DuSP), and other genes for the normal prostate tissue, primary prostate carcinomas (PC) of 84 patients, 7 xenografts, and 5 carcinoma cell lines. Analysis of 96 profiles revealed eight clusters of genes coexpressed in PC (coefficient of correlation r > 0.7).

[Molecular portrait of human kidney carcinomas: the gene expression profiling of protein-tyrosine kinases and tyrosine phosphatases which controlled regulatory signals in the cells].

Hybridization with cDNA arrays was used to obtain expression profiles of 214 protein-tyrosine kinase, protein-tyrosine phosphatase, dual-specific phosphatase, and other genes for kidney carcinomas (KC) and normal kidney tissues of 34 patients and for seven carcinoma cell lines. Computer analysis revealed three clusters of genes coexpressed in KC. A proliferating-cell gene cluster included MET, VIM, MYC, TOP2A, PCNA, etc.

[Effect of insulin on lipid peroxidation in the erythrocyte membranes of patients with insulin dependent diabetes mellitus].

The spin-probe techniques has been used to study insulin-membranes interaction in erythrocytes by insulin dependent diabetes mellitus. The lipid peroxidation has been initiated by FeSO4-ascorbate. It was found the insulin defend the lipids in control by peroxidation, but no effect in insulin dependent diabetes mellitus erythrocytes.

[Structural organization of red blood cell membranes in pregnant women with gestosis].

Activated lipid peroxidation (LPO) was found to occur with decreased antioxidative activity (AOA) in gestosis, by deteriorating the structural and functional properties of cell membranes. This enhanced the permeability of the phospholipid bilayer and altered the function of membranous proteins and the synthesis of biologically active agents, by involving the systems responsible for microcirculatory homeostasis. Enhanced membrane hydrophilicity and increased LPO and AOA are typical of the second and third trimesters of normal pregnancy.

[Average daily values of the isotopic carbon composition in expired air and urea in human urine in normal states and in some endocrine disorders].

Daily average carbon isotope composition of CO2 of expired air and urine urea in patients being in different hormonal-metabolic states was determined. The observations were carried out under conditions of clinic to provide standard diet, and the same daily and food taking regimes. In all persons, a substantial increase enrichment in 12C of CO2 of expired air (by 3-6%) and 13C of urine urea (by 3-5%) relative to carbon of food was revealed.

[Short-period oscillations of isotopic carbon in CO2 from expired air in different human functional states].

The examination of diurnal variations in carbon isotope composition of CO2 under different hormonal-metabolic states in man revealed short-period inphase oscillations with a period of about 2-3 h. The oscillations occur throughout 24 h and their period is little dependent on the functional state of the organism. In endocrine pathologies, substantial differences in oscillation amplitude are observed.

[Mechanisms of functional disorders of the membrane receptor apparatus in pregnant women with insulin-dependent diabetes mellitus].

Studies of membrane receptor system in patients with insulin-dependent diabetes mellitus revealed that insulin resistance in pregnant patients with type I diabetes is caused by disordered cellular sensitivity to threshold physiological and submaximal insulin doses, whereas the maximal doses of the hormone normalize glucose consumption by the cells. High insulin doses intensify lipid peroxidation, normalize the status of membranous proteins, reduce the number of thiol groups, reduce AOA level in membranes, and, hence, reduce membranous capacity to bind active peroxide radicals. Structural and functional changes in red cell membranes are associated with reduced affinity of insulin receptors, reduction in the number of insulin-binding sites in membranes, this disordering intracellular effects of insulin.

[Recording of surface potential changes in erythrocyte membranes from patients with insulin-dependent diabetes mellitus using a spin probe method].

Changes in surface potential of erythrocyte membranes were studied by a positively charged analog as a spin probe. Insulin-dependent diabetes mellitus (IDDM) patients demonstrated a significant increase (by 16 +/- 2.0 mV) in negative surface potential as compared to the control.

[Daily changes in the carbon isotope composition of CO2 in expired air of persons with various metabolic disorders].

Daily variations of the aspiration CO2 carbon isotope content (CIC) were studied at three metabolic states: norm, insulin-dependent diabetes (IDD), and obesity. The groups of subjects were studied in a clinic during 24 hours. Analysis of the delta 13C daily curves of the exhaled air CO2 allowed us to find the link between variations of CIC with daily rhythms in the organism and with metabolic shifts at IDD and obesity.

[Fetoplacental hormone complex measurements to predict fetal and neonatal viability in insulin-dependent diabetes mellitus].

A dynamic follow-up of 79 pregnant patients with insulin-dependent diabetes mellitus was carried out. Specific fetoplacental hormonal profiles were detected and analyzed in complex with glycemic levels, this permitting the authors obtain new prognostic criteria of fetal and newborn viability. The results helped optimize the policy of following up such patients and the developed prediction regulations allowed preventive measures aimed at improvement of the future baby health status to be carried out before clinical manifestation of fetal intrauterine suffering.

[The determination of microalbuminuria by immunoenzyme analysis].

The authors have defined the optimal conditions for inhibitory type enzyme immunoassay of microalbuminuria. The range of albumin detection is 30 to 250 micrograms/ml. The optimal time of antigen binding with antibody is 45 min, the lower threshold level of albumin detection 0.

[Structural changes in erythrocyte membranes in diabetes mellitus using spin labelled fatty acids].

Microviscosity and polarity of erythrocyte membranes of the blood of patients suffering from diabetes were studied by ESR using spin-labeled fatty acids. Structural changes were discovered 0.6-0.

[Hormonal-metabolic disorders in children with diabetes mellitus in the preclinical stage of diabetic retinopathy].

A relationship was established between alterations in vessels of the fundus of the eye and diabetes mellitus standing, the character of metabolic disorders, hereditary load with regard to cardiovascular diseases. The risk factors of the development of the preclinical stage of diabetic retinopathy are as follows: hereditary load with regard to cardiovascular diseases, parship, an increase of the content of lactate, a reduction of the content of 2,3-diphosphoglycerophosphate, a rise of the content of glycosylated hemoglobin, high content of cholesterol esters in red blood cell membranes, of free cholesterol and malonic dialdehyde in blood serum, a decline of the level of phospholipids and a rise of cardiolipin content in red blood cell membranes, and increase of blood serum cortisol.

[Effect of glucagon and short-term fasting on the interrelationship of cellular and humoral immune responses].

The effect of subcutaneous administration of 25 micrograms/kg of glucagon and 2-day fasting (as a natural model of hyperglucagonemia) on the accumulation of antibody-forming splenocytes and the expression of a delayed-type hypersensitivity reaction (DTHR) in response to intraperitoneal immunization with sheep erythrocytes at various doses were studied in experiments on random-bred albino mice. A suppressing effect of the hormone on antibody genesis at optimum doses and its stimulating effect at suboptimum doses were revealed. The latter effect took place against a background of DTHR suppression.