The Dome Technique, an Option for Massive Anterosuperior Medial Acetabular Bone Loss: A Retrospective Case Series.

Background: Acetabular bone loss in revision total hip arthroplasty can be very challenging even for fellowship-trained surgeons. Although it is uncommon, massive anterosuperior medial defects may be encountered, but treatment options have been limited and better ones are needed.

Questions/purposes: The primary purpose of this case series is to describe a novel surgical treatment, which we call the dome technique, that can be used to address these challenging defects.

Preliminary Report: Pediatric Femur Fractures: Single Incision Intramedullary Stabilization Technique.

Background: Pediatric femoral shaft fractures are common injuries that have seen a trend toward surgical stabilization. Traditionally these fractures have been stabilized with flexible intramedullary nails performed through a 2-incision technique. We proposed that a single incision technique would offer equivalent outcomes with the potential for improved cosmesis and a reduced infection risk.

Multicentre evaluation of the Boehringer Mannheim/Hitachi 717 analysis system.

We conducted an European multicentre trial to assess the performance of the new Boehringer Mannheim/Hitachi 717 analysis system. The photometer response was linear up to an absorbance of 2.8.

Demonstration of a combined deficiency of xanthine oxidase and aldehyde oxidase in xanthinuric patients not forming oxipurinol.

Genetic heterogeneity has been suggested in xanthinuria from the hitherto unexplained ability of some patients with this hereditary disorder to convert allopurinol to its active metabolite oxipurinol--an activity generally attributed to xanthine oxidase. This study provides evidence that the enzyme aldehyde oxidase is also deficient in xanthinuric patients not converting allopurinol to oxipurinol, whereas a xanthinuric patient with normal formation of oxipurinol had normal aldehyde oxidase activity. It is concluded that the enzyme aldehyde oxidase is the principal enzyme responsible for the formation of oxipurinol in man.

Isocratic ion-pair high-performance liquid chromatographic methods for the determination of uroporphyrin and coproporphyrin type II and IV isomers in human urine.

Urinary porphyrins of porphyric patients were isolated as their methyl esters by using a simple, modified thin-layer chromatographic system. Existing methods for the isocratic ion-pair high-performance liquid chromatographic separation of uroporphyrin and coproporphyrin isomers were decisively improved by elevating the column temperatures, changing the types of columns used and modifying the eluent compositions. These techniques were applied to the determination of the isomeric distribution of uroporphyrins and coproporphyrins isolated from urines of patients in the acute or latent phase of acute intermittent porphyria.

Digitalis intoxication and treatment with digoxin antibody fragments in renal failure.

Severe digitalis intoxication today is preferentially treated by intravenous infusion of Fab fragments of digoxin antibodies (Digitalis Antidot BM). The kinetics of Fab fragments in the circulation are well known when kidney function is normal or slightly impaired. There are no data available, however, in complete renal failure.

Evaluation of a new chemiluminescence immunoassay for the determination of human chorionic gonadotropin in serum.

A new commercially available chemiluminescence immunoassay for the quantitative measurement of human chorionic gonadotropin and its beta-subunit in serum was compared with the enzyme immunoassay used in our routine laboratory. Human chorionic gonadotropin was determined in serum from pregnant women, as well as from women with abortus imminens, suspected ectopic pregnancies or with molar pregnancies. The new human chorionic gonadotropin assay was also evaluated in combination with an automatic sample processor for distributing samples to the antibody-coated wells of the microtitre plates.

Detection of non-typical porphyrin isomers in human urines by ion-pair reversed-phase high-performance liquid chromatography.

An improved ion-pair reversed-phase high-performance liquid chromatographic system has been developed for the separation of uroporphyrin isomers I, II and III, whereas the isomers III and IV could not be resolved. Application of this method to the analysis of urines from porphyric patients indicated the presence of small amounts of the non-typical uroporphyrin isomer II. The questionable presence of the isomer IV was confirmed by acid-catalyzed decarboxylation to the corresponding coproporphyrin isomers, which were completely separated by a modified ion-pair method at elevated column temperatures.

Investigation of the performance of the ES 600 Enzymun-Test system. A multicentre study.

The ES 600 sample-selective multibatch analyser was subjected to a multicentre evaluation in six laboratories in accordance with ECCLS guide-lines. During the 3-month trial, five Enzymun-Test diagnostics (T4, TBG, Digoxin, CEA and TSH)1) were measured at 25 degrees C. The study yielded the following results: 1.

A multicentre evaluation of the Boehringer Mannheim/Hitachi 704 analysis system.

The selective multitest Boehringer Mannheim/Hitachi 704 analysis system was examined according to the ECCLS guidelines in a multicentre evaluation involving four laboratories. Ten routine parameters, covering most of the application settings of the instrument, were measured in the respective laboratory at temperatures 25, 30 or 37 degrees C. The trial lasted four months and gave more than 40,000 data.

Analytical performance of the random access analyser Hitachi 737. A multicentre evaluation.

The selective multitest analyser Hitachi 737 was examined according to the ECCLS guidelines in a multicentre evaluation involving 4 laboratories. Twenty routine parameters, including the electrolytes, sodium, potassium and chloride, were measured at 37 degrees C. All of the measured values were included for evaluation without correcting for outliers.

[Immunophenotyping of lymphocyte subpopulations using monoclonal antibodies in an analytical flow cytometer: evaluation of the method and reference values].

Analytical flow cytometers which are easy to operate, and monoclonal antibodies specific for cell surface determinants of lymphocytes, offer a simple and rapid method for the identification of the immunological phenotype of lymphocyte subsets. We investigated mononuclear cells isolated from EDTA-blood bv density gradient centrifugation. The intraassay precision (n = 12) of the determination by means of monoclonal antibodies directed against T-cells (Leu 4), T-helper/inducer-cells (Leu 3 a, b), T-suppressor/cytotoxic cells (Leu 2 a) and cells of the 'NK'-phenotype (Leu 7) varied between 2.

[Computer-assisted findings on protein electrophoresis on cellulose acetate film].

Until a short time ago efficient computer assisted reporting of electrophoretograms was not possible owing to the lack of appropriate technology. By integrating a fully mechanized electrophoresis system into the laboratory data processing system of a centralized institute of clinical chemistry, the separation and the interpretation of results can be performed for 300 samples per day. The interpretation is based upon available patient data (specified clinical diagnoses, previous results), calculated fractions and analysis of the electrophoretic curve in the area of albumin and the beta- and gamma-globulin fraction.

A data processing system adapted to the special needs of the emergency laboratory.

A data processing system for the emergency laboratory was integrated in our clinical laboratory computer system, its prime objective being the service requirements of the laboratory. It included the possibility of simultaneous optical reading of request forms and on-line capturing, processing, and printing of laboratory test data. Priority request forms, which allow the clinician to specify the interval by which emergency test results must be available, are registered by an optical reader and arranged according to urgency by the computer.

Analysis of free stool porphyrins by high-performance liquid chromatography.

The determination of stool porphyrins is necessary for the diagnosis of some porphyrias in clinical laboratories. Quantitative methods for the analysis of faeces for porphyrins are unpleasant and difficult to perform. An extraction and ion-pair reversed-phase high-performance liquid chromatographic procedure is described for the separation and determination of individual free stool porphyrins.

[Optimization of sample splitting in a centralized clinical chemical institute (author's transl)].

Mechanized sample splitting machines controlled by a laboratory data processing system have been realized in only a few centralized laboratories. Bottlenecks and mistakes in sample processing are avoided by means of direct machine readable identification and parallel splitting of the secondary tubes. Our previous experience has shown that a strategy for sample splitting has to go far beyond these basic functional requirements.

Cluster-oriented discriminant analysis; taxonomic classification of the thyroid function.

A computer assisted procedure for the diagnosis of thyroid diseases, based on seven clinical chemical parameters, is proposed. The population studied consisted of 592 consecutive outpatients with a tentative diagnosis of thyroid disease. Thyroxine, triiodothyronine, T3 uptake test, TSH before and after TRH application, its difference and thyroxine binding globulin have been determined.

Evaluation of a new valproic acid enzyme immunoassay and comparison with a capillary gas-chromatographic method.

A new homogeneous immunoassay (EMIT) for valproic acid was evaluated. Besides testing the manual version of this enzyme immunoassay, we also developed two mechanized procedures for centrifugal analyzers (the CentrifiChem and the COBAS system), which take less time and are more precise than the manual method. Within-assay precision (CV) was 4.

Diagnosis of porphyrias by ion-pair high-performance liquid chromatography.

Ion-pair reversed-phase high-performance liquid chromatography together with fluorescence detection is useful in the analysis of urinary porphyrin carboxylic acids. The sensitive and quantitative detection facilitates the clinical diagnosis of porphyrias. The method described permits the detection of porphyrins down to 0.