Haematological and Chosen Biochemical Parameter Assessment of the Antioxidant System in Red Deer () Blood in Early and Late Pregnancy.

Introduction: The aim of this study was to investigate changes in haematological parameters and the antioxidant system in the early and late pregnancy of red deer ().

Material And Methods: Blood samples were collected from 30 red deer females 50 days after impregnation and 40 days before calving. Complete blood counts and stained blood smears were assessed.

The occurrence of dynamic structural disorders in the pharynx and larynx, at rest and during exercise, in horses diagnosed with mild and moderate Equine Asthma (Inflammatory Airway Disease).

Unlabelled: The goal of the present study was to establish the occurrence of structural disorders in the larynx and pharynx during treadmill exercise tests in horses diagnosed with Equine Asthma (EA). Investigation was performed in 29 horses, patients of the Equine Clinic of the Warsaw University of Life Sciences in Poland, admitted with poor exercise performance. Upper and lower airway examinations were performed in all patients revealing both mild to moderate Equine Asthma (13 horses), and no lower airway abnormalities (16 animals).

Serum paraoxonase 1 activity and lipid metabolism parameter changes in Dachshunds with chronic mitral valve disease. Assessment of its diagnostic usefulness.

Chronic mitral valve disease, which is frequently diagnosed in Dachshunds, leads to structural, hemodynamic and redox state changes in dogs. The aim of this study was to investigate serum paraoxonase 1 (PON1) activity and lipid metabolism in different disease stages. Standardized PON1 activity (PON1/HDL ratio) was lower in asymptomatic dogs, B1 and B2 Stages when compared to healthy ones and symptomatic Dachshunds in Stage C (ACVIM classfication).

Pulmonary adiaspiromycosis in the Eurasian beaver (Castor fiber) inhabiting Poland.

Adiaspiromycosis is a rare fungal infection caused by saprophytic fungi Emmonsia spp. (type Ascomycota) occurring especially in small free-living mammals. The aim of this study was to evaluate the occurrence of histopathological lesions asscociated with adiaspiromycosis in the Eurasian beaver inhabiting Poland.

ProANP as a screening biomarker for hypertrophic cardiomyopathy in Maine coon cats.

Unlabelled: The aim of this study was to determine if atrial natriuretic peptide can be used as an early screening tool for hypertrophic cardiomyopathy in Maine coon cats.

Animals: The study was performed in 43 Maine coon cats of both sexes, aged 11 to 92 months. Clinical and echocardiographic examinations were done and proANP serum concentrations were measured every three months over a period of one year (each cat had a total of five examinations).

Immunosuppression in Dogs During Mammary Cancer Development.

Cancer immunosuppression that facilitates tumor progression and metastasis evolves by development of an immunosuppressive network. The aim of this study was to assess this network in dogs with benign or malignant tumors with or without confirmed metastasis. The authors showed that the number of various T cell subpopulations was constant during tumor development; however the number of regulatory T cells (Tregs) was significantly higher in tumor-bearing dogs than in healthy individuals.

Serum paraoxonase 1 (PON1) activity and lipid metabolism parameters changes in different production cycle periods of Holstein-Friesian, Polish Red and Norwegian breeds.

We investigated the measurement of paraoxonase 1 (PON1), as a potential marker of redox state changes in dairy cows, its involvement in lipid metabolism and compared it with superoxide dismutase (SOD) activity changes. We also evaluated lipid metabolism parameters associated with dairy production. PON1 paraoxonase and arylesterase acitvities, SOD activity, beta-hydroxybutyrate (BHB), uric acid (UA), high density lipoprotein (HDL), low density lipoprotein (LDL), cholesterol and triglyceride concentrations were measured in Holstein-Friesian, Polish Red and Norwegian breeds serum in two production cycles.

Cytological evaluation of tracheal aspirate and broncho-alveolar lavage fluid in comparison to endoscopic assessment of lower airways in horses with recurrent airways obstruction or inflammatory airway disease.

The aim of the present study was to compare the grade of discharge accumulation in the tracheal lumen, area of tracheal bifurcation, main bronchi and the tracheal septum thickness with the cytology of the tracheal aspirate (TA) and broncho-alveolar lavage fluid (BALF) in horses with recurrent airways obstruction and inflammatory airway disease from those horses. This study was conducted on 96 horses with RAO, 139 horses with IAD and 10 control horses. In all the horses, both clinical and endoscopic examinations were performed.

Histological Evaluation of Selected Organs of the Eurasian Beavers (Castor fiber) Inhabiting Poland.

There is a general scarcity of data on the histological structure of major organs in the Eurasian beaver (Castor fiber). This study presents the histological characteristics of beaver organs such as the liver, spleen, cardiac muscle, lungs and kidneys. Tissue samples were collected from 21 beavers and analysed.

Immunohistochemical evaluation of superoxide dismutase (Cu/Zn SOD) concentrations in erythrocytes of dairy cattle and farm-raised deer by a computer-assisted analysis of microscopic images.

The effectiveness of the immunohistochemical method in determining Cu/Zn SOD concentrations in red blood cells of dairy cattle and farm-raised deer was evaluated by a computer-assisted analysis of microscopic images and scanning technique. Superoxide dismutase (Cu/Zn SOD) concentrations in erythrocytes were determined in smears of whole blood samples collected from 16 Polish Holstein-Friesian cows and 22 farm-raised deer in spring. Mouse anti-bovine SOD (Cu-Zn) monoclonal antibodies (2F5, Serotec) were used in 1:50 dilution.

Usefulness of the assessment of discharge accumulation in the lower airways and tracheal septum thickening in the differential diagnosis of recurrent airway obstruction (RAO) and inflammatory airway disease (IAD) in the horse.

The aim of the present study was to use endoscopic evaluation to compare the grade of accumulation of discharge in the lower airways of horses with recurrent airway obstruction (RAO) and inflammatory airway disease (IAD), and to estimate the usefulness of endoscopic evaluation in differential diagnosis of these diseases. Endoscopic evaluation consists of: the amount of discharge in the lower part of the trachea and tracheal bifurcation, and the assessment of tracheal septum thickness. This study was performed on 248 horses, 10 were healthy, 97 were diagnosed as RAO, and 141 were diagnosed as IAD.

Serum paraoxonase-1 activity of dairy Holstein-Fresian cows in different lactation stages--preliminary study.

The objective of this study was to investigate paraoxonase-1 (PON-1) activity in different lactation stages. The study was conducted on Holstein--Friesian dairy cows in 2nd and 3rd lactation. A significant decrease in paraoxonase activity was found in the postpartum period and during peak of lactation.

Hematological parameters in relation to age, sex and biochemical values for mute swans (Cygnus olor).

The knowledge of the correct morphological and biochemical parameters in mute swans is an important indicator of their health status, body condition, adaptation to habitat and useful diagnostic tools in veterinary practice and ecological research. The aim of the study was to obtain hematological parameters in relation to age, sex and serum biochemistry values in wild-living mute swans. We found the significant differences in the erythrocyte count, hematocrit, hemoglobin concentration and erythrocyte sedimentation rate in relation to age of mute swans.

TransEndoscopic Gastric SubMucosa Islet Transplantation (eGSM-ITx) in pigs with streptozotocine induced diabetes - technical aspects of the procedure - preliminary report.

Background: Islets and pancreas transplantation have become standard treatments of patients with diabetic complications. However pancreas transplantation is associated with high incidence of complications and the long-term results of islet transplantation are still unsatisfactory. Loss of pancreatic islets grafts is caused not only by immunological reactions but also due to the site of grafting and IBMIR.

Concentration of ascorbic acid in the blood of cows with subclinical mastitis.

The aim of the investigations was evaluation of ascorbic acia concentration in the blood or cows in the subclinical form of mastitis. The research was conducted on 56 cows. The cows were divided into 4 groups: A, B, and C with subclinical form of mastitis caused by Staphylococcus aureus, Streptococcus agalactiae and Escherichia col, and D control.

Role of antioxidants in the protection against oxidative stress in cattle--trace elements and enzymatic mechanisms (Part 3).

The most important enzymatic mechanisms which protect an organism against oxidative stress are superoxide dismutase (SOD), peroxidase (Px), e.g. glutathione peroxidase (GSH-Px) and ascorbate peroxidase, catalase and glutathione reductase.

Role of the antioxidants in the protection against oxidative stress in cattle--nonenzymatic mechanisms (Part 2).

Antioxidative status consists of two mechanisms: nonenzymatic and enzymatic mechanisms. Nonenzymatic mechanisms are composed of antioxidants, scavengers of free radicals, transition metal ions, sequester transition metal ions, albumins, ceruloplasmin, and metallothioneins. On the other hand, enzymatic mechanisms are composed of superoxide dismutase (SOD), peroxidase, catalase and reductase.

Role of transition metals ion and reactive oxygen species in biological oxidation in cattle (part 1).

Technological advances in last decades of XX centuries were well utilised in the studies of biological oxidation processes. Biological oxidation can lead to the oxidative stress and subsequently to the cell damage of animal organisms leading to many diseases. Free radical processes taking place in cattle under pathological conditions.

Antioxidant parameters of horses according to age, sex, breed and environment.

Antioxidant defences interact to form an integrated system. There is no comprehensive and uniform view on issues concerning the antioxidant status in horses. Therefore, the aim of the present study was to estimate antioxidant parameters in horses of different age, sex and breed as well as environment and relationship between different antioxidants.

Effect of Baypamun on blood leucocytes in normal and dexamathasone treated goats.

The aim of this study was to determine the effect of Baypamun on selected lymphocyte subpopulations and granulocyte phagocytic activity mediated by lectin-like receptors in goats of normal immune status and in goats experimentally immunosuppressed with dexamethasone. Eighteen goats in total were used. Blood samples were collected 24 h before immunomodulation and 5 and 10 days after immunomodulation.

The effect of lecithin supplementation on the biochemical profile and morphological changes in the liver of rats fed different animal fats.

Sixty-four Wistar rats were divided into 8 equal groups and kept for 36 days in individual boxes. Three of the groups were given full synthetic diets containing various animal fats: beef tallow, pork fat or fish oil. A control group was fed the diets without the fat.

Flow cytometry analysis of milk and peripheral blood cells from goats during lactation.

Cells from goat's milk and peripheral blood taken during the lactation period were analysed by flow cytometry. The investigated cells were populations of leucocytes, lymphocyte subpopulations (T, T-helper, T-cytotoxic, B, WC1-N2) and all MHC class II positive cells. Labelling of cells was performed on whole blood and milk cell suspensions.

The effect of the addition of oil preparation with increased content of n-3 fatty acids on serum lipid profile and clinical condition of cats with miliary dermatitis.

The aim of this study was to determine the influence of an oil preparation with increased n-3 fatty acids content on the serum lipid profile and clinical condition of cats with miliary dermatitis. The studies were performed on 22 cats of various breeds and both sexes, aged between 3 and 7 years, that had been fed a balanced diet, based on beef meat for the previous 6 months. The animals were divided into four groups: I, five clinically healthy cats; II, five clinically healthy cats, receiving 1 ml of oil preparation with food for 6 weeks; III, seven nonpruritic cats with miliary dermatitis, without treatment; and group IV, five nonpruritic cats with miliary dermatitis, receiving 1 ml of oil preparation per day with food for 6 weeks.

The effect of persistent chlorinated hydrocarbons on the secretion of estradiol and progesterone by bovine granulosa cells in vitro.

The role of persistent chlorinated hydrocarbons (PCH) in the reproductive disorders in ruminants is not well documented. In the present study we have examined the effect of these compounds and their metabolites on the secretion of estradiol (E2) and progesterone (P4) by bovine granulosa cells in vitro. Granulosa cells were isolated from large follicles (> or = 8 mm diameter) by gently washing the internal follicle wall.