Bifidobacterium and Lactobacillus Probiotics and Gut Dysbiosis in Preterm Infants: The PRIMAL Randomized Clinical Trial.

Importance: The effects of probiotic interventions on colonization with resistant bacteria and early microbiome development in preterm infants remain to be clarified.

Objective: To examine the efficacy of Bifidobacterium longum subsp infantis, Bifidobacterium animalis subsp lactis (BB-12), and Lactobacillus acidophilus (La-5) probiotics to prevent colonization with multidrug-resistant organisms or highly epidemic bacteria (MDRO+) and to shape the microbiome of preterm infants toward the eubiotic state of healthy full-term infants.

Design, Setting, And Participants: The multicenter, double-blinded, placebo-controlled, group sequential, phase 3 Priming Immunity at the Beginning of Life (PRIMAL) randomized clinical trial, conducted from April 2018 to June 2020, included infants with gestational age of 28 to 32 weeks at 18 German neonatal units.

Resilience and stability of the CF- intestinal and respiratory microbiome during nutritional and exercise intervention.

Background: Impaired respiratory and intestinal microbiome composition is linked to cystic fibrosis lung disease severity. In people with cystic fibrosis (pwCF), regular exercise is recommended to delay disease progression and preserve a stable lung function. An optimal nutritional status is vital for best clinical outcomes.

Compounding Risks Caused by Heat Exposure and COVID-19 in New York City: A Review of Policies, Tools, and Pilot Survey Results.

The Coronavirus Disease 2019 (COVID-19) pandemic changed many social, economic, environmental, and healthcare determinants of health in New York City (NYC) and worldwide. COVID-19 potentially heightened the risk of heat-related health impacts in NYC, particularly on the most vulnerable communities, who often lack equitable access to adequate cooling mechanisms such as air conditioning (AC) and good quality green space. Here, we review some of the policies and tools which have been developed to reduce vulnerability to heat in NYC.

Meconium Microbiome of Very Preterm Infants across Germany.

Meconium constitutes infants' first bowel movements postnatally. The consistency and microbial load of meconium are different from infant and adult stool. While recent evidence suggests that meconium is sterile , rapid colonization occurs after birth.

BET bromodomain inhibitors regulate keratinocyte plasticity.

Although most acute skin wounds heal rapidly, non-healing skin ulcers represent an increasing and substantial unmet medical need that urgently requires effective therapeutics. Keratinocytes resurface wounds to re-establish the epidermal barrier by transitioning to an activated, migratory state, but this ability is lost in dysfunctional chronic wounds. Small-molecule regulators of keratinocyte plasticity with the potential to reverse keratinocyte malfunction in situ could offer a novel therapeutic approach in skin wound healing.

Containment of a Large SARS-CoV-2 Outbreak Among Healthcare Workers in a Pediatric Intensive Care Unit.

Objective: Healthcare workers (HCWs) are particularly exposed SARS-CoV-2 because they are critical in preventing viral transmission and treating COVID-19 patients. Within HCWs, personnel of intensive care units (ICUs) are at the forefront of treating patients with a severe course of COVID-19 infection and therefore represent an extremely vulnerable group. Thus, our objective is to contribute to establish means of infection control protecting HCWs in the frontline of the current pandemic.

Utilising Low Complexity CNNs to Lift Non-Local Redundancies in Video Coding.

Digital media is ubiquitous and produced in ever-growing quantities. This necessitates a constant evolution of compression techniques, especially for video, in order to maintain efficient storage and transmission. In this work, we aim at exploiting non-local redundancies in video data that remain difficult to erase for conventional video codecs We design convolutional neural networks with a particular emphasis on low memory and computational footprint.

Opportunities and Challenges for Filling the Air Quality Data Gap in Low- and Middle-Income Countries.

Given the millions of people suffering from air pollution, filling the air quality monitoring gap in low- and middle-income countries has been recognized as a global challenge. To meet this challenge and make it work will require private enterprise, multiple levels of government, international organizations, academia and civil society to work together toward the common goal of characterizing, understanding better, and then reducing, the air pollution that causes sickness and preventable death for millions of people each year in lowand middle-income countries around the world. This article offers concrete next steps on how to make progress toward increasing air quality monitoring using a combination of emerging technologies, adaptation to country-specific conditions, and building capacity towards the development of lasting institutions.

Detection of acute periodontal pain from physiological signals.

Objective: To investigate the feasibility of the detection of brief orofacial pain sensations from easily recordable physiological signals by means of machine learning techniques.

Approach: A total of 47 subjects underwent periodontal probing and indicated each instance of pain perception by means of a push button. Simultaneously, physiological signals were recorded and, subsequently, autonomic indices were computed.

Cost-effective large-scale expression of proteins for NMR studies.

We present protocols for high-level expression of isotope-labelled proteins in E. coli in cost-effective ways. This includes production of large amounts of unlabeled proteins and C-methyl methionine labeling in rich media, where yields of up to a gram of soluble protein per liter of culture are reached.

Inhibition of prenylated KRAS in a lipid environment.

RAS mutations lead to a constitutively active oncogenic protein that signals through multiple effector pathways. In this chemical biology study, we describe a novel coupled biochemical assay that measures activation of the effector BRAF by prenylated KRASG12V in a lipid-dependent manner. Using this assay, we discovered compounds that block biochemical and cellular functions of KRASG12V with low single-digit micromolar potency.

Transfection of insect cell in suspension for efficient baculovirus generation.

Baculovirus (BV) mediated insect cell expression system utilizes transfection as a first step to introduce recombinant baculovirus DNA into insect cells. Many labs are still relying on the conventional liposome based transfection method in adherent culture. Here we describe a more efficient method that can replace the existing method.

Changing the Conversation with Home Care Clients.

Traditional home care delivery involves executing tasks for clients within a limited timeframe. Five years ago, when surveys of clients of Toronto Central Community Care Access Centre (TC CCAC) showed the lowest client experience levels across the 14 CCACs in Ontario, TC CCAC and its contracted Service Provider Organizations developed and implemented Changing the Conversation, a philosophy and framework that focuses on asking clients "what is most important" to them and then using that information as the basis for how care is delivered. Changing the Conversation has made a measurable difference to clients' experiences and has now expanded to other parts of the province and the country.

Why participation matters for air quality studies: risk perceptions, understandings of air pollution and mobilization in a poor neighborhood in Nairobi, Kenya.

Objectives: With high urbanization rates, Sub-Saharan Africa is facing growing problems of poor air quality in its cities. We make a case for participatory approaches in air quality studies especially including those living in poor neighborhoods who may be particularly at risk from this trend.

Study Design: We used collaboration with a community based organization, interviews, focus group discussions and a community forum.

Optimization of a Dibenzodiazepine Hit to a Potent and Selective Allosteric PAK1 Inhibitor.

The discovery of inhibitors targeting novel allosteric kinase sites is very challenging. Such compounds, however, once identified could offer exquisite levels of selectivity across the kinome. Herein we report our structure-based optimization strategy of a dibenzodiazepine hit 1, discovered in a fragment-based screen, yielding highly potent and selective inhibitors of PAK1 such as 2 and 3.

Affordable uniform isotope labeling with (2)H, (13)C and (15)N in insect cells.

For a wide range of proteins of high interest, the major obstacle for NMR studies is the lack of an affordable eukaryotic expression system for isotope labeling. Here, a simple and affordable protocol is presented to produce uniform labeled proteins in the most prevalent eukaryotic expression system for structural biology, namely Spodoptera frugiperda insect cells. Incorporation levels of 80% can be achieved for (15)N and (13)C with yields comparable to expression in full media.

Auto-inducing media for uniform isotope labeling of proteins with (15)N, (13)C and (2)H.

Auto-inducing media for protein expression offer many advantages like robust reproducibility, high yields of soluble protein and much reduced workload. Here, an auto-inducing medium for uniform isotope labelling of proteins with (15)N, (13)C and/or (2)H in E. coli is presented.

Insect cell culture in reagent bottles.

Growing insect cells with high air space in culture vessel is common from the early development of suspension cell culture. We believed and followed it with the hope that it allows sufficient air for optimal cell growth. However, we missed to identify how much air exactly cells need for its growth and multiplication.

Thriving public-private partnership to fortify cooking oil in the West African Economic and Monetary Union (UEMOA) to control vitamin A deficiency: Faire Tache d'Huile en Afrique de l'Ouest.

Background: In sub-Saharan Africa, more than 42% of children are at risk for vitamin A deficiency, and control of vitamin A deficiency will prevent more than 600,000 child deaths annually. In the West African Economic and Monetary Union (UEMOA), an estimated 54.3% of preschool-age children are vitamin A deficient and 13% of pregnant women have night blindness.

Influence of molecular ordering on electrical and friction properties of ω-(trans-4-stilbene)alkylthiol self-assembled monolayers on Au(111).

The electrical and friction properties of ω-(trans-4-stilbene)alkylthiol self-assembled monolayers (SAMs) on Au(111) were investigated using atomic force microscopy (AFM) and near edge X-ray absorption fine structure spectroscopy (NEXAFS). The sample surface was uniformly covered with a molecular film consisting of very small grains. Well-ordered and flat monolayer islands were formed after the sample was heated in nitrogen at 120 °C for 1 h.

Identity theft in community mental health patients: two case reports.

Identity theft is a serious problem in the United States, and persons with enduring mental illnesses may be particularly vulnerable to becoming victims of this crime. Victims of identity theft experience a variety of consequences that include financial loss and serious emotional distress. Little is known about the impact of identity theft on individuals with mental illnesses.

Designing a non-volatile imaging switch for mass-persistent, chemically amplified photolithography: a model study.

An acid catalysed rearrangement that transforms a bicyclic lactone into a phenolic carboxylic acid has been tested for potential use in chemically amplified microlithographic imaging.

Timing and localization of movement-related spectral changes in the human peri-Rolandic cortex: intracranial recordings.

Event-related spectral power (ERSP) was measured from intracranial EEG and used to characterize the time-course and localization of the Rolandic mu rhythms in 12 patients during the delayed recognition of words or faces (DR) and the discrimination of simple lateralized visual targets (LVD). On each trial, the subject decided whether to make manual response (Go) or not (NoGo). ERSP increased on both Go and NoGo trials in peri-Rolandic regions of all subjects with a peak latency of approximately 330-ms poststimulus and duration of 260 ms during the DR task.

Early widespread cortical distribution of coherent fusiform face selective activity.

Intracranial EEG was analyzed from 16 pharmaco-resistant epilepsy patients. Subjects participated in memory tasks for faces and/or words. Face-selective coherence increases were found between the fusiform gyrus and temporal, parietal, and frontal cortices at 160-230 ms poststimulus onset.