Publications by authors named "Kloke A"

Contamination of foods is a public health hazard that episodically causes thousands of deaths and sickens millions worldwide. To ensure food safety and quality, rapid, low-cost and easy-to-use detection methods are desirable. Here, the LabSystem is introduced for integrated, automated DNA purification, amplification and detection.

View Article and Find Full Text PDF

Assay automation is the key for successful transformation of modern biotechnology into routine workflows. Yet, it requires considerable investment in processing devices and auxiliary infrastructure, which is not cost-efficient for laboratories with low or medium sample throughput or point-of-care testing. To close this gap, we present the LabTube platform, which is based on assay specific disposable cartridges for processing in laboratory centrifuges.

View Article and Find Full Text PDF

Cyclic electrodeposition of platinum and copper enables the fabrication of high surface area electrodes (roughness factors of >3000) by multiple alternation of alloy co-deposition and dealloying of copper from the just-fabricated alloy layers. The underlying processes, resulting electrode structures, and their applicability to potentially implantable glucose fuel cells are discussed.

View Article and Find Full Text PDF

Porous platinum is of high technological importance due to its various applications in fuel cells, sensors, stimulation electrodes, mechanical actuators and catalysis in general. Based on a discussion of the general principles behind the reduction of platinum salts and corresponding deposition processes this article discusses techniques available for platinum electrode fabrication. The numerous, different strategies available to fabricate platinum electrodes are reviewed and discussed in the context of their tuning parameters, strengths and weaknesses.

View Article and Find Full Text PDF
Article Synopsis
  • The study explores the use of laccase enzymes from Trametes versicolor for enhancing the performance of biofuel cell cathodes through direct electron transfer from carbon electrodes.
  • Comparative tests showed that laccase significantly improves open circuit potentials and overall performance of various carbon-based electrodes, with current densities varying based on electrode type at a specific voltage.
  • Findings suggest that carbon nanotubes and porous carbon tubes perform worse than graphite felt and carbon nanofibers when adjusted for surface area, indicating the need for future research into enzyme interaction with electrode surfaces and potential improvements in electrode fabrication.
View Article and Find Full Text PDF

Often, reproducible investigations on bio-microsystems essentially require a flexible but well-defined experimental setup, which in its features corresponds to a bioreactor. We therefore developed a miniature bioreactor with a volume in the range of a few millilitre that is assembled by alternate stacking of individual polycarbonate elements and silicone gaskets. All the necessary supply pipes are incorporated as bore holes or cavities within the individual elements.

View Article and Find Full Text PDF

Contamination of soil by toxic substances, in particular from hazardous waste and air pollution, is not only an important environmental medical problem but also has far-reaching effects on the community. Environmental toxicological assessment is based on the different types of use taking the paths of ingestion into consideration. In the following article the scheme by Eikmann and Kloke systematizes this approach.

View Article and Find Full Text PDF