H, C, and N resonance assignments of the amyloidogenic peptide SEM2(49-107) by NMR spectroscopy.

It has been shown that human seminal fluid is a major factor in enhancing HIV activity. The SEM2(49-107) peptide is a product of cleavage after ejaculation by internal prostheses of the semenogelin 2 protein, expressed in seminal vesicles. It is established that the peptide SEM2(49-107) forms amyloid fibrils, which increase probability of contracting HIV infection.

Crystal structure of the GDP-bound GTPase Era from Staphylococcus aureus.

GTPase Era from Staphylococcus aureus belongs to the TRAFAC superfamily of the TrmE-Era-EngA-EngB-Septin-like GTPases class and plays a significant role in the vital activity of this pathogenic microorganism as a maturation factor of the 30S ribosome subunit. However, the functions of this protein are not fully understood, making it a promising object for further study. Here, the 2.

Extent of N-Terminus Folding of Semenogelin 1 Cleavage Product Determines Tendency to Amyloid Formation.

It is known that four peptide fragments of predominant protein in human semen Semenogelin 1 (SEM1) (SEM1(86-107), SEM1(68-107), SEM1(49-107) and SEM1(45-107)) are involved in fertilization and amyloid formation processes. In this work, the structure and dynamic behavior of SEM1(45-107) and SEM1(49-107) peptides and their N-domains were described. According to ThT fluorescence spectroscopy data, it was shown that the amyloid formation of SEM1(45-107) starts immediately after purification, which is not observed for SEM1(49-107).

[ORF19.2286 Gene: Isolation and Purification of Deoxyhypusine Hydroxylase from the Human Pathogenic Yeast Candida albicans].

Candida albicans (C. albicans) is a fungal pathogen that causes infections of the wet body surfaces and the blood in immunocompromised patients or individuals with imbalanced microflora. Since the cases of clinically meaningful candidosis are on the rise, efficient С.

The data of heterologous expression protocol for synthesis of N, C-labeled SEM1(68-107) peptide fragment of homo sapiens semenogelin 1.

The semenogelin 1 protein is secreted in the seminal vesicles. After ejaculation it is split into small peptide fragments using internal proteases. It was shown that the fragments SEM1(45-107), SEM1(49-107), SEM1(68-107) (SEM1(86-107) form amyloid fibrils, which increase the possibility of HIV infection.

Summary and analysis of Russian and international requirements for the safe management of very low level radioactive waste and production waste containing very low active radionuclides.

This article gives an overview of Russian legislation and international recommendations on the safe management regulation of the accumulated very low level radioactive waste (RW), provides the comparison of Russian and international criteria for RW attribution to this or that class and also the existing radiation safety requirements. This article also deals with the difficulties in management of production waste containing radionuclides. Criteria and standards were selected from international (ICRP and IAEA publications) and Russian (Federal Law on Management of Radioactive Waste, Government Decree on Radioactive Waste Classification, The Basic Sanitary Rules of Radiation Safety) documents for comparative analysis.

In vitro Reconstitution of the S. aureus 30S Ribosomal Subunit and RbfA Factor Complex for Structural Studies.

Ribosome-binding factor A (RbfA) from Staphylococcus aureus is a cold adaptation protein that is required for the growth of pathogenic cells at low temperatures (10-15°C). RbfA is involved in the processing of 16S rRNA, as well as in the assembly and stabilization of the small 30S ribosomal subunit. Structural studies of the 30S-RbfA complex will help to better understand their interaction, the mechanism of such complexes, and the fundamental process such as 30S subunit assembly that determines and controls the overall level of protein biosynthesis.

Oligomerization of the antimicrobial peptide Protegrin-5 in a membrane-mimicking environment. Structural studies by high-resolution NMR spectroscopy.

Protegrin pore formation is believed to occur in a stepwise fashion that begins with a nonspecific peptide interaction with the negatively charged bacterial cell walls via hydrophobic and positively charged amphipathic surfaces. There are five known nature protegrins (PG1-PG5), and early studies of PG-1 (PDB ID:1PG1) shown that it could form antiparallel dimer in membrane mimicking environment which could be a first step for further oligomeric membrane pore formation. Later, we solved PG-2 (PDB ID:2MUH) and PG-3 (PDB ID:2MZ6) structures in the same environment and for PG-3 observed a strong d NOE effects between residues R18 and F12, V14, and V16.

Antimicrobial peptide protegrin-3 adopt an antiparallel dimer in the presence of DPC micelles: a high-resolution NMR study.

A tendency to dimerize in the presence of lipids was found for the protegrin. The dimer formation by the protegrin-1 (PG-1) is the first step for further oligomeric membrane pore formation. Generally there are two distinct model of PG-1 dimerization in either a parallel or antiparallel β-sheet.

Structure of pravastatin and its complex with sodium dodecyl sulfate micelles studied by NMR spectroscopy.

The aim of this work was to study the mechanisms of interaction between pravastatin and cell membranes using model membranes (sodium dodecyl sulfate micelles) by nuclear magnetic resonance spectroscopy methods. On the basis of the nuclear magnetic resonance experiments, it was established that pravastatin can form intermolecular complexes with sodium dodecyl sulfate micelles by the interaction of its hydrophilic groups with the polar surface of the micelle. Conformational features of pravastatin molecule were also studied.

[Prevention of eventration in diffuse suppurative processes in small pelvis].

The study was devoted to the problem of postoperative eventrations in gynaecological practice. Coaptation removable sutures were applied in order to prevent similar complications. Operations were performed in the cases of desolate condition of purulent disease of the uterus and uterine appendages with diffuse suppurative peritonitis.

[Supervision over selection and exploitation of individual protective skin devices on objects and territories serviced by Federal Medical Biologic Agency].

The article covers main principles underlying sanitary epidemiologic supervision over selection and exploitation of individual protective skin devices on objects of Federal Agency on Nuclear Energy. The authors consider radiation hazards influencing humans; provide specific medical and technical requirements to individual protective skin devices, which are monitored during preventive and current sanitary epide miologic supervision and determining peculiarities of use of individual protective skin devices by personnel working in radiation-hazardous conditions.

Comparison of diets in two native Chukotka populations and prevalence of ischemic heart disease risk factors.

Epidemiological studies of nutrition and the prevalence of ischemic heart disease risk factors were carried out comparing native Markovskaya populations living in the tundra and coastal areas. There was lower prevalence of IHD risk factors among 30-59 year old males in coastal villages than in a comparable population residing in the tundra. This finding can be partially explained by analysis of the diets of both regions.

[Characteristics of the nutrition and blood lipids of the native residents of Chukot Peninsula and the Buryat ASSR].

Simultaneous epidemiologic investigations of representative samplings of the male population, aged from 30 to 59 years, were carried out in Chukot Peninsula and the Buryat ASSR in 1981-1982 and 1985-1986 years. The programme of the study included standard questioning to reveal angina of effort according to the WHO Cardiologic Questionnaire, ECG recording at rest, arterial pressure measuring (twice), anthropometry, biochemical blood assay (the content of total cholesterol, triglycerides and high density lipoprotein cholesterol). Actual nutrition was studied by the method of "daily reproduction" in 104 residents of Markovskaya tundra (85.

[Nutrition characteristics and the incidence of dyslipoproteinemia and ischemic heart disease among the population of the Buryat ASSR].

The paper presents the data on dietary habits, blood lipid composition, and prevalence of coronary heart disease in native and non-native populations from Buryatia. They were obtained by using epidemiological (assessment of amounts of the food ingredients consumed) and biochemical (measurement of blood levels of total cholesterol, triglycerides, and high density lipoprotein cholesterol) methods. Differences were demonstrated in dietary habits between the native and non-native populations from Buryatia.

[Nutrition and levels of risk factors for ischemic heart disease in 30- to 59-year-old men of Novosibirsk and resident newcomers of Chukotka].

Data on actual diets and major coronary risk factors are presented for residents of a district in Novosibirsk and nonaboriginal residents of Chukotka. Epidemiologic (quantitative assessment of dietary habits, questioning to detect angina of effort), biochemical (assays of plasma cholesterol, triglycerides, high density lipoprotein cholesterol) and physiologic (arterial BP, height and weight measurements) methods were used. Differences are demonstrated between the diets common for Novosibirsk and nonaboriginal Chukotka residents, which show correlation to differences in the incidence of major coronary risk factors.

[Nutrition and risk factors of ischemic heart disease in men of the Chukot Autonomous Region].

The relationship between the nutritional status and the major risk factors of CHD has been studied in the indigenous and nonindigenous inhabitants of the Chukot Autonomous Region. The nutrition of the indigenous tundra inhabitants who show a significantly lower risk factors as compared with nonindigenous citizens living there for over ten years is characterized by a significantly higher proportion of protein of animal origin, a lower calorie intake in general and that of fat, in particular, a lower consumption of starch-containing products and a high consumption of refined sugar. A larger proportion of polyunsaturated fatty acids in food is markedly associated with reduced levels of cholesterol, beta-lipoprotein cholesterol and the diastolic blood pressure in Chukchi inhabitants living on the seacoast as against Chukchi living on the mainland.