ALDOSTERONE SYNTHASE GENE С-344Т POLYMORPHISM AS A RISK FACTOR OF EARLY LEFT VENTRICULAR REMODELING IN YOUNG HYPERTENSIVE PATIENTS WITH OBESITY.

The purposes of the research were to study the prevalence of C-344T polymorphism and the distribution of aldosterone synthase gene (CYP11B2) genotypes, to analyze the association of aldosterone concentration with aldosterone synthase gene genotypes, to study the features of left ventricular hypertrophy (LVH) by echocardioscopy and identify their association with different genotypes of the aldosterone synthase gene in young patients with arterial hypertension (AH), depending on the presence or absence of concomitant obesity (or overweight). 123 young patients with essential AH (18-44 years old) were examined, the average age was (32,83±0,58) years old, the male/female ratio was 72/51 respectively. All patients were divided into 3 groups: group 1 (n=41) with normal body weight; group 2 (n=40) with overweight; group 3 (n=42) -with obesity.

[ORGAN-SPECIFIC AND ORGAN-NONESPECIFIC AUTOANTIBODIES AND DAMAGE OF ORGANS AND SYSTEMS AT SJOGREN'S SYNDROME].

The aim of the study was to determine the presence of various organ-specific and organ - nonspecific autoantibodies and their association with Sjogren's syndrome - one of the most pervasive autoimmune disorders that affects entire body. The etiology of Sjogren's syndrome is not clearly understood. It may be due combination of factors: inherited, hormonal, infection from virus.

Comparison of structures and cytotoxicity of mupirocin and batumin against melanoma and several other cancer cell lines.

To determine the computer-predicted anticancer activity of mupirocin and to compare its activities with those determined for another polyene antibiotic, batumin. Molecular docking, cytotoxicity assays, cell microscopy and cell cycle progression were studied in cancer and nontumorigenic cell lines. Cytotoxicity of mupirocin against several cancerous cell lines was detected with the highest one (IC = 5.

Promising anticancer activity of batumin: a natural polyene antibiotic produced by Pseudomonas batumici.

Aim: To determine the computer-predicted anticancer activity of antibiotic batumin.

Materials & Methods: Cytotoxicity assays, cell morphology microscopy and cell cycle progression were studied in cancer and nontumorigenic cell lines. An in vivo experiment on Lewis lung carcinoma (3LL)-transplanted mice was conducted to evaluate potential antimetastatic.

Mitochondrial genomes reveal an east to west cline of steppe ancestry in Corded Ware populations.

From around 4,000 to 2,000 BC the forest-steppe north-western Pontic region was occupied by people who shared a nomadic lifestyle, pastoral economy and barrow burial rituals. It has been shown that these groups, especially those associated with the Yamnaya culture, played an important role in shaping the gene pool of Bronze Age Europeans, which extends into present-day patterns of genetic variation in Europe. Although the genetic impact of these migrations from the forest-steppe Pontic region into central Europe have previously been addressed in several studies, the contribution of mitochondrial lineages to the people associated with the Corded Ware culture in the eastern part of the North European Plain remains contentious.

Еcology, Systematics and Antibiotic Activity of Pseudomonas batumici and Alteromonas macleodii in Connection with Analysis of their Genome Structure.

New data about ecology, systematics and synthesis of biologically active substances of the type strain Pseudomonas batumici UCM-321 producing the antibiotic batumin, highly effective against staphylococci, and of Alteromonas macleodii strains, as representatives of marine species widely inhabiting the world ocean, was obtained based on a complex analysis of their biological properties and genomic structure. Analysis of taxonomic data indicated that P. batumici is a novel species.

[FATTY ACID COMPOSITION ALTEROMONAS-LIKE BACTERIA ISOLATED FROM THE BLACK SEA WATER].

Alteromonas macleodii strains isolated from the Black sea water were similar in their fatty acids composition with the type strain of this species. Analysis of lipid composition of 10 A. macleodii strains isolated from the deep and surface water layers in different World ocean regions including the Black sea water has shown that the deep and surface isolates of this species formed two groups different in their fatty acids profiles.

Prospects of a new antistaphylococcal drug batumin revealed by molecular docking and analysis of the complete genome sequence of the batumin-producer Pseudomonas batumici UCM B-321.

Meticillin-resistant Staphylococcus aureus (MRSA) is a serious public health threat causing outbreaks of clinical infection around the world. Mupirocin is a promising anti-MRSA drug, however mupirocin-resistant strains of S. aureus are emerging at an increasing rate.

[The taxonomic study of the strain Bacillus sp. UCM B-7404--phytopathogenic fungae antagonist].

The polyphasic taxonomic analysis of strain Bacillus sp. UCM B-7404 active against phytopathogenic fungi and producing extracellular phytohormones, lytic enzymes and lipopeptide antifungal compounds has been carried out. The basic cell wall fatty acids presented by branched iso- and anteiso- C15:0 and C17:0 acids, contained 65-77% of the average pool.

Taxonomic analysis of the Streptomyces sp. 2435 strain, a producer of antimicrobial substances.

The study of the taxonomic status of the antimicrobial substances producer strain Streptomyces sp. 2435 was conducted The nucleotide sequence of 16S rRNA gene of the strain was determined and deposited in the Genbank (No JN129837) database. Results of morphological, biochemical and cell wall fatty acids content analyses, evaluation of biosynthesis features of Streptomyces sp 2435, together with the phylogenetic analysis have provided the basis to identify this strain as Streptomyces albus.

[Antifungal and antiviral substances of Pseudomonas chlororaphis subsp. aureofaciens strains--components of gaupsin].

Phenazine-1-carboxylic, 2-hydroxy-phenazine-carboxylic acid and 2-hydroxy-phenazine active against phytopathogenic fungi were detected in fermentation broth of Pseudomonas chlororaphis subsp.aureofaciens strains UCM B-111 and UCM B-306--components of insectofungicide biopreparation gaupsin using chromato-mass-spectrometric methods; strain B-306 produced antifungal antibiotic pyrrolnitrin together with phenazines. Supernatants of fermentation broth of P chlororaphis subsp.

[Geno- and phenotypic characteristic of Bacillus strains--components of endosporin].

Endosporin is used in veterinary for the prophylaxis and treatment of disbacteriosis, intestinal infections, festering wounds and postpartum pyoinflammatory complications in agricultural animals. The probiotic is based on two Bacillus strains which inhibit growth of a broad spectrum of pathogenic microorganisms and synthesise proteolytic enzymes and other biologically active secondary metabolites, particularly - polysaccharides. The activity of these two strains was supplementary.

Peculiarities of Alteromonas macleodii strains reflects their deep/surface habitation rather than geographical distribution.

The phenotypic, chemotaxonomic and genetic peculiarities of 5 deep strains of Alteromonas macleodii (isolated from Adriatic and Ionian Sea water from a depth of 1,000-3,500 m) and 5 strains of the same species isolated from the surface layer of Aegean, Andaman, Black Sea and Atlantic Ocean water near the British shore have been studied. Electron microscopy has shown that the deep strains' cells were, on average, two times longer (2.1±0.

Does the applicability of Bacillus strains in probiotics rely upon their taxonomy?

The taxonomic position and biological activities of two Bacillus strains used in veterinary probiotics were studied in this work. These microorganisms inhibit growth of a broad spectrum of pathogenic cultures. They synthesize proteolytic enzymes and other biologically active metabolites, and to some extent, supplement each other with probiotic activities.

Pseudomonas batumici sp. nov., the antibiotic-producing bacteria isolated from soil of the Caucasus Black Sea coast.

Four novel strains of saprophytic bacteria were isolated from the soil samples collected in the moist subtropics region (the Black Sea coast of the Caucasus) and studied using methods of polyphasic taxonomic analysis. Microorganisms were Gram-negative, oxidase positive, aerobic, rod-shaped motile bacteria that produced antibiotic named batumin with high and selective activity against staphylococci; its total formula was C30H48N2O7 Phylogenetic analysis of 16S rRNA gene sequences (1376 bp, accession number in Genbank--JF306642) indicated that the isolates belonged to the gamma-Proteobacteria formed a separate branch within the genus Pseudomonas and had 98% 16S rRNA gene sequence similarity with Pseudomonas gingeri. The latter essentially differed from the studied strains in its phenotypic characteristics.

In vitro antifungal activity of phenylheptatriyne from Bidens cernua L. against yeasts.

In vitro antifungal activity of phenylheptatriyne from Bidens cernua L. (Asteraceae) was studied using broth macrodilution method against 125 strains of yeasts including 104 clinical and other isolates of Candida spp. (C.

[Analysis of relation between structure and antibiotic activity of Batumin].

Antimicrobial activity of six derivatives and products of chemical modification of antistaphylococcal antibiotic Batumin and one natural analog of this antibiotic formed by the strain-producer has been investigated. Not a single substance, which could exceed Batumin or be equal to it as to activity and selectivity in respect of staphylococci was detected among the studied compounds. It was shown that a unique antimicrobial effect of Batumin was determined by several peculiarities of its molecule: presence of double bonds, group--CONH2, as well as carboxyl and hydroxyl groups.

[Antimicrobial spectrum of antibiotic batumin].

Antibacterial activity of antibiotic batumin, isolated from Pseudomonas genus has been studied using CLSI standard methods. Batumin was highly active against all studied strains belonging to 10 species of Staphylococcus genus (minimal inhibitory concentration (MIC)--0.25-0.

[Influence of cultivation conditions on the antibiotic activity in "Pseudomonas batumici"--batumin producer].

The optimum parameters of cultivation of "Pseudomonas batumici"--a producer of antistaphylococcal antibiotic batumin has been established. The periodical process of culture development and antibiotic synthesis has been studied. Conditions of batumin biosynthesis are the temperature 23-25 degrees C, aeration 0.

[The epidemiological surveillance of poliomyelitis in the Ukraine].

Correlation has been revealed between the circulation of polioviruses among the population and environmental objects. A rise in the circulation of polioviruses, type I, was caused by a decrease in collective immunity to this virus type. Water supply and sewage system purification was found ineffective with respect to enteroviral contamination.