[Influence of maternal factor on motor behavior and lipid turnover in rats exposed to severe psychoemotional stress].

Adverse condition of rat development during the prenatal period (rearing by mother survived brain injury) or during the early post-natal period (cross fostering), causes impairment of motor behavior and a lipid turnover in adult rats. Such rats under severe stress showed decrease of adaptation (as expressed or low indexes of motor impellent behavior and lipid metabolism) in comparison with rats that were grown up by own mother. The psychoemotional stress caused the most expressed deficient behavior in males that were grown up by mothers with brain injury (decrease in serum level of HC and HDL and depressively - similar behavior).

[Hypolipidemic activity of trimethylglycine].

Hypolipidemic properties of a native trimethyl derivative of glycine, trimethylglycine (TMGl), were studied in hyperlipidemic rats and guinea pigs. The administration of TMGl to the hyperlipidemic rats and guinea pigs produced a pronounced hypolipidemic effect. A positive action of TMGl on the lipid profile of blood serum of the experimental animals was observed as manifested by a decrease in the level of cholesterol of low density lipoproteins (LDL) and an increase in the cholesterol level of high density lipoproteins (HDL).

[Disturbances in the function of cardiomyocyte ryanodine receptors of spontaneously hypertensive rats revealed by 4-chloro-m-cresol].

The rates of Ca2+ release from sarcoplasmic reticulum in response to the activation of ryanodine receptors by 4-chloro-m-cresol in cardiomyocytes of three rat lines: spontaneously hypertensive (SHR) and normotensive WKY, and Wistar rats during five weeks of their growth and development have been studied to reveal differences in the function of ryanodine receptors at different stages of hypertension. Whereas the efflux of Ca2+ from sarcoplasmic reticulum in Wistar and WKY rat cardiomyocytes decreased in response to 4-chloro-m-cresol, an abrupt rise in the rate of [Ca2+]i increase was observed in myocytes of spontaneously hypertensive rats after 17 days of development. A correlation between this phenomenon and the occurrence of genetic defect of ryanodine receptors in SHR seems improbable because we did not register any differences in the rates of Ca2+ release from sarcoplasmic reticulum by the action of 4-chloro-m-cresol in concentrations 0.

[Experience in treating chronic periodontal diseases with vilprafene (josamycin)].

Forty-two patients with chronic generalized periodontitis were treated. Thirty-five patients presented with exacerbation of the disease of second-third degree of severity, clinically manifesting with hyperemia, edema of the gingival mucosa with pyorrhea from the gingivodental pouches, and mobility of teeth of the second-third degree; exacerbation of the process with abscesses round 1-2 teeth was observed in 4 patients, abscesses in the periodontal bone in 2, and edematous hypertrophic gingivitis in 1 patient. All patients were treated with vilprafene.

[Effects of parathyroid hormone and parathyroid hypertensive factor on myocardial mechanical properties in normotensive rats].

After a 10-minute incubation, both the parathyroid hypertensive factor (PHF) and parathyroid hormone (PTH) reduced myocardial contractility and accelerated myocardial relaxation. Effects of the PTH and PHF on myocardial inotropic characteristics were of opposite directions.

[Ability to induce an experimental hypercholesteremia in rats with reduced ability for drinking conditioning].

A diet rich in cholesterol caused individual differences in its accumulation in the blood of unbred male rats. High sensitivity of rats to hypercholesterinemia is reciprocally related to drinking conditioning.

[Hypertensive activity of blood plasma of WKY rats with a calcium deficiency in drinking water].

The effects of low (8.0 mg/l) and normal (80 mg/l) Ca2+ water diets on systolic blood pressure (SBP) and hypertensive plasma activity were studied. At the end of the 16-week experimental period SBP in the low-Ca2+ group of animals was higher than in the control group after the water treatment: 161 sigma 9 and 120 +/- 4 mm Hg respectively.

[The hypolipidemic activity of natural substances].

Experiments on guinea pigs and rats have revealed that thiazole derivatives of isoflavone has a beneficial effects on disturbed lipid and lipoprotein metabolism. New phospholipid complexes enriched with vitamins have been demonstrated to produce a hypolipidemic effect. Sulfated chitosans decrease lipid infiltration of the liver and elevate serum high density lipoprotein levels in rats.

[Binding of cholesterol by apolipoproteins A-I and E].

It was shown that cholesterol can interact with some guanidine group-containing compounds (guanidine proper, arginine, metformine and dodecylguanidine bromide) as well as with the arginine-rich proteins--apoproteins A-1 and E. In the latter case this interaction results in the formation of cholesterol-apoprotein complexes. Analysis of such complexes revealed that one apo-A-1 molecule binds 17-22, whereas one apo-E molecule--30-35 sterol molecules, which approximately correspondence to the amount of arginine residues in these proteins.

[The role of guanidine groups of arginine residues of Na, K-ATPase and glutamate dehydrogenase in an interaction with cholesterol].

Cholesterol was studied in experiments in vitro for its effect on the activity of Na, K-ATPase of the synaptic brain membranes of rats and a crystalline preparation of glutamate dehydrogenase from the liver mitochondria of a bull. Cholesterol decreased the activity of the above enzymes. When blocking guanidine groups of arginine residues of Na, K-ATPase and glutamate dehydrogenase the inhibiting action of cholesterol was absent.

[Participation of apolipoprotein E in the transport of cholesterol esters].

It is established in the in vitro experiments that subfraction of HDL3 is able of accepting cholesterol from the atherosclerosis-afflicted aorta intima. Apoprotein E has no effect on the acceptance of cholesterol from the intima by HDL3 particles. The role of the protein under its joint incubation with the aorta intima and HDL3 is reduced to the uptake of cholesterol esters from HDL3-particles enriched by cholesterol.

[Isolation and regeneration of the protoplasts of the streptomycete producers of actinomycins C and X].

Protoplasts of S. michiganensis, S. chrysomallus and Streptomyces sp.

[Effect of a hypercapnic stimulus on the postinspiratory phase of the respiratory cycle in the anesthetized cat].

The technique of differentiation of the envelope of total activity of the diaphragm motoneurons in anesthetized cats revealed that augmentation of the chemostimulus induced a linear growth of the latetinspiration inhibition which increased after bilateral vagotomy. Duration of the postinspiration phase does not depend on the level of activation of the chemoreceptors and lung stretch receptors. Vagotomy induces a discrete elongation of the postinspiration phase due, apparently, to effects from the structures of rostral pons.

[Inhibition of lipoprotein lipolysis by polyarginine and evaluation of the mechanism of its interaction with lipoprotein lipase].

It was found that polyarginine (Mr 40 000-60 000) is a strong inhibitor of the lipoprotein lipase activity in vivo and in vitro. The inhibitory effect in vivo was observed after a single intravenous injection of 0.85-3.

[Interaction of peptides with cholesterol].

Interaction of cholesterol with apoproteins A-I and E was studied in absence of phospholipids in vitro. As shown by two methods - ultracentrifugation in density gradient of KBr and an extraction technique - an apoprotein E molecule bound 30-35 molecules of cholesterol and a molecule of A-I bound 17-22 molecules of the sterol. If the primary structure of apoprotein A-I and the fragments of apoprotein E are involved in consideration, the hydrophilic reaction appears to occur between the hydroxyl group of cholesterol and a guanidine group of the protein arginine residues as well as hydrophobic reaction - between side aliphatic chain of the sterol and the branched-chain amino acid, present in the protein at a distance of 4 amino acid residues from the arginine residue.

[The role of the structural organization of apoprotein E in cholesterol binding].

The ability of some proteins to bind cholesterol was accompanied by a decrease of turbidity of aqueous cholesterol suspensions and correlated with a quantity of arginine residues in them. Maximum clearing of aqueous cholesterol suspensions at the addition of proteins containing equimolar arginine concentrations was observed in the presence of apoproteins E and A-I. Optical rotatory dispersion spectra of apoprotein E, polyarginine and histone H3 have shown the influence of sterol on the secondary structure of apoprotein E only.

[Mathematical model of the mechanism of respiratory rhythmogenesis].

Contemporary ideas of the mechanism of respiratory rhythmogenesis are considered. A mathematical model of these mechanisms is described as well as the results of its study underlying a proposed scheme of neuronal network which is able to maintain a regular alternation of respiratory phases within the wide range of physiological states. Three neuronal pools constitute the basis of the proposed network: the inspiratory neurons I alpha and I beta (the former excite the latter) and the added pool of expiratory neurons Ee which can be excited by the I beta neurons and inhibits the I alpha neurons.

[Interaction of cholesterol with polypeptides and amino acids].

Using absorbance spectra at 185-225 nm, the interaction of cholesterol and its methyl and acetic esters with some amino acids, guanidine, metformine and two polypeptides-polyarginine and polylysine was studied. In aqueous and alcoholic media (pH 6-7) the largest shift of the absorption maximum in the presence of non-esterified cholesterol was observed in the compounds containing guanidinio groups (guanidine, metformine, arginine, polyarginine) and, in a lesser degree, the compounds containing epsilon-amino groups (lysine, polylysine). The cholesterol esters produced a marked shift in the absorption maxima of arginine, lysine, polyarginine and polylysine in a spirituous medium (as compared to an aqueous medium).

[Effect of a hyperbaric nitrogen-oxygen atmosphere on respiratory center activity].

In anesthesized cats inspiratory activity of the respiratory center at first increased and then decreased with increases in total pressure of the nitrogen-oxygen atmosphere to 7, 11, and 21 atm (at normal PO2). The effort made by respiratory muscles increased gradually. These changes were augmented by hypercapnia.

[Responses of the respiratory system to changes in gas density at different inspiratory flow rates].

The effect of altered gas density on the respiratory function was investigated in relation to the velocity of gas flows in airways. In the normal man breathing of normoxic He-O2 (with a density of 0,34 as compared to air) slightly decreased and inhalation of SF6-O2 (with a density of 4.2) significantly increased the metabolic rate of 1 1 ventilation.