Value based maternal and newborn care requires alignment of adequate resources with high value activities.

Background: Evidence based practice has been associated with better quality of care in many situations, but it has not been able to address increasing need and demand in healthcare globally and stagnant or decreasing healthcare resources. Implementation of value-based healthcare could address many important challenges in health care systems worldwide. Scaling up exemplary high value care practices offers the potential to ensure values-driven maternal and newborn care for all women and babies.

Intrapartum and neonatal mortality in primary midwife-led and secondary obstetrician-led care in the Amsterdam region of the Netherlands: A retrospective cohort study.

Objective: To compare intrapartum- and neonatal mortality and intervention rates in term women starting labour in primary midwife-led versus secondary obstetrician-led care.

Design: Retrospective cohort study.

Setting: Amsterdam region of the Netherlands.

Pregnancy in a patient with autosomal-dominant polycystic kidney disease and congenital hepatic fibrosis.

This case report describes a pregnancy in a patient with autosomal-dominant adult polycystic kidney disease and congenital hepatic fibrosis, a very rare and problematic combination. In particular, hypertension and renal dysfunction caused problems during this pregnancy. Peritoneal dialysis became necessary.

Tubal damage after intratubal methotrexate treatment.

We report on a case of seriously hampered tubal repair 6 months after local MTX treatment. Histopathological examination showed destruction of tubal mucosa and remnants of the EP.

Cloning of the replication gene P of bacteriophage lambda: effects of increased P-protein synthesis on cellular and phage DNA replication.

A restriction fragment of lambdaDNA carrying the P gene was cloned in the high copy number plasmid RSF2124. Cells harbouring this new plasmid RSF2124/lambdaE complement lambdaPam80 phage. A lac promoter-operator region (lacP), produced by EcoRI digestion of plasmid pKB252, was inserted into RSF2124/lambdaE such that induction of the lac promoter by IPTG or lactose leads to increased production of the P gene product.

Roles of bacteriophage lambda gene products O and P during early and late phases of infection cycle.

Ring-to-ring (early) replication of bacteriophage lambda DNA was blocked after heat inactivation of the P protein. Rolling circle (late) replication continued for several rounds at the rate reached when the temperature shift was carried out. The same differential effect was observed after inhibition of RNA or protein synthesis during the two different phases of replication.