Assessment of Immunogenicity of Adjuvanted Quadrivalent Inactivated Influenza Vaccine in Healthy People and Patients With Common Variable Immune Deficiency.

Recent addition to vaccines of adjuvants has been actively used to enhance the immunogenicity. However, the use of adjuvants for the development of quadrivalent inactivated influenza vaccines (QIV) is currently limited. The aim of this study was to examine immunogenicity of adjuvanted QIV in healthy people and patients with primary immune deficiency-common variable immune deficiency (CVID).

Large-scale high-quality 2D silica crystals: dip-drawing formation and decoration with gold nanorods and nanospheres for SERS analysis.

High-quality colloidal crystals (CCs) are important for use in photonic research and as templates for large-scale plasmonic SERS substrates. We investigated how variations in temperature, colloid concentration, and dip-drawing parameters (rate, incubation time, etc) affect the structure of 2D CCs formed by highly monodisperse silica nanoparticles (SiNPs) synthesized in an l-arginine solution and regrown by a modified Stöber method. The best quality 2D CCs were obtained with aqueous 12 wt% colloids at a temperature of 25 °C, an incubation time of 1 min, and a drawing rate of 50 mm min(-1).

[The influence of subclinical thyroid dysfunction on cardiac hemodynamic and electrophysiological parameters in patients with coronary heart disease and paroxysmal atrial fibrillation].

The study included 58 patients with CHD and paroxysmal atrial fibrillation divided into 2 groups depending on thyroid hormone and thyrotropin levels. Group 1 was comprised of 24 patients with subclinical hypothyroidism, group 2 of 18 ones with subclinical hyperthyroidism. Control group consisted of l6 patients with CHD, paroxysmal atrial fibrillation and normal thvroid function.

[The role of dopamine in the regulation of the interaction between nervous and immune systems in multiple sclerosis].

A complex neurological and neuropsychological examination, including the detection of depression, was conducted in 71 patients with multiple sclerosis aged from 23 to 62 years (mean age 43 ± 9 years). An immunobiochemical study included the analysis of expression of CD80 and CD86 on monocytes and B-cells, expression of TLR2 and TLR4 on monocytes, expression of D3 and D5 dopamine receptors on monocytes and B-cells, using flow cytometry, and the determination of Il-6, Il-10, IL-12p40 and dopamine plasma levels as well as vanillylmandelic acid (VMA, dopamine metabolite) urine levels measured with immunoenzyme assay. The results suggest the involvement of dopamine in the pathogenesis of depression in MS as assessed by dopamine and its metabolites levels.

[The dependence of Toll-receptor expression on pathomorphological features of nasal polyps in patients with polypous rhinosinusitis].

This work was designed to study expression of Toll 1-10 receptors on the surface of cells present in inflammatory infiltrate from nasal polyps and peripheral blood of the patients with polypous rhinosinusitis. It was shown that the intensity of expression depended on the pathomorphological characteristics of nasal polyps. Tissues removed from the patients with polyps of the oedematous type contained more Toll-10 positive cells and showed enhanced expression of Toll-5 receptors on monocytes and lymphocytes, Toll-3 receptors on monocytes, granulocytes, and lymphocytes, and Toll-9 receptors on granulocytes.

[The relationship between eosinophilia and parameters of systemic and local adaptive immunity in patients with polypous rhinosinusitis].

Results of the study on adaptive immunity in patients with polypous rhinosinusitis (PRS) proved to depend on the degree of eosinophilia in the peripheral blood. The patients were allocated to two groups, one comprised of those having up to 150 eosinophils per 1 microliter the other of the patients with a higher eosinophil concentration. Patients of the former group had a significantly reduced number of CD3+, CD4+, CD8+, and CD20+cells in the peripheral blood that may indicate the necessity of administering immunotropic agents.

Orchid-associated bacteria produce indole-3-acetic acid, promote seed germination, and increase their microbial yield in response to exogenous auxin.

Germination of orchid seeds is a complex process. In this paper we focus on interactions between the host-plant and its bacterial partners via indole-3-acetic acid (IAA). Originally isolated from the roots of the epiphytic orchid Dendrobium moschatum, the strains of Rhizobium, Microbacterium, Sphingomonas, and Mycobacterium genera were among the most active IAA producers.

[Hormones and hormone-like substances of microorganisms: a review].

Data from the literature on the ability of microorganisms to form plant hormones have been reviewed. The substances covered include abscisic acid, ethylene and other compounds with phytohormone-like properties (brassinosteroids, oligosaccharines) and analogues of animal neurotransmitters (biogenic amines). Pathways whereby the substances are metabolized and their effects on the development and activity (physiological and biochemical) of the microorganisms are considered.

[Microbial producers of plant growth stimulators and their practical use: A review].

The ability of pro- and eukaryotic microorganisms to synthesize growth-stimulating phytohormones is reviewed, with emphasis on pathways of biosynthesis of these compounds and their effects on physiological and biochemical properties of the producers. Phytohormones are viewed as specific mediators in interactions between various organisms inhabiting the same ecological niche, the biological role of which is not limited to processes taking place in plants. In addition to setting forth theoretical aspects of this problem, the review underscores the need to utilize such producer microorganisms in plant cultivation and biotechnology.

[TNF-induced apoptosis and necrosis in myeloleukemia cells HL-60 is regulated by reactive oxygen metabolites depending on a cell cycle phase].

Unlabelled: TNF is the known death receptor ligand which induce apoptosis and necrosis. Reactive oxygen metabolites, ceramide (synthesized de novo and as a product of sphingomyelin cycle), and caspases have been implicated as potential mediators of cell death. Their mechanistic relationship remains to be elucidated.

Competitive Specificity Analysis of Natural Antibodies against Epitope of Bacterial Cell Wall Peptidoglycoan: Glucosaminylmuramyl Dipeptide Carrying Adjuvant Activity.

The natural antibodies against glucosaminylmuramyl dipeptide (GMDP), the epitope of peptidoglycan of bacterial cell wall, isolated from human serum by thermal extraction possess a capability to cross-react with determinant of glycan chain - tetrasaccharide consisting of N-acetylglucosamine and N-acetylmuramic acid. The intensity of interaction of natural anti-GMDP-antibodies with specific ligand is significantly higher than with tetrasaccharide. The natural antibodies against tetrasaccharide carry properties of heteroclitic antibodies, i.

Affinity of Serum Natural Antibodies against Peptidoglycan Bacterial Cell Component with Adjuvant Activity.

We studied affinity of natural antibodies of human serum against glucosaminylmuramyl dipeptide (GMDP), the epitope peptidoglycan bacterial cell wall component which carries adjuvant activity. Antibodies against GMDP were isolated from the blood sera of healthy donors using thermal extraction of antibodies from specific ligand on plastic. Determination of the dissociation constant (K(d)) showed equal K(d) in the serum and affinity-purified anti-GMDP-antibodies, i.

Synthesis and organotropism of 3H-dihydro derivatives of the cyanobacterial peptide hepatotoxin nodularin.

Tritium-labelled dihydro derivatives of the cyanobacterial peptide hepatotoxin nodularin were prepared by reduction with sodium boro[3H]hydride. The optimised reaction gave two dihydronodularin stereoisomers which were purified by high-performance liquid chromatography with a mobile phase of methanol-0.7% sodium sulfate (6:4) and a C(18) stationary phase.

[Flow laser cytometry for evaluation of the human immune system].

Some flow laser cytometry (FLC) techniques intended for studies of the immune system cells are reviewed. A widespread analytical method is the phenotyping of lymphocytes by the markers they express. The use of FLC permits the evaluation of practically all functional parameters of immunocompetent cells.

[Influence of bioactive dietary supplement--dietary unsaturated fats--on immune status of patients with myocardial ischemia and impaired glucose tolerance].

Immune status measured by the flow cytrometric analysis under the ischemic heart disease and IHD with impaired glucose tolerance has been found to be altered in B cells as well as in T lymphocytes in some cases. Having received the diet supplemented by PUFA "Eicolen", IHD patients present with the restored immune status. Patients with IHD and impaired glucose tolerance did not present with any significant modifications in immune status after PUFA "Eicolen" supplementation.

[A competitive analysis of the specificity of natural antibodies to the epitope of the bacterial cell wall peptidoglycan: the glucosaminylmuramyl dipeptide possessing adjuvant activity].

Natural antibodies to glucosaminyl dipeptide (GMDP), a component of bacterial cell-wall peptidoglycan, isolated from the serum of normal donors by thermal extraction, are capable of cross reaction with the determinant of the glycan chain: tetrasaccharide consisting of N-acetylglucosamine and N-acetylmuramic acid. The intensity of the interaction of natural anti-GMDP antibodies with a specific ligand is considerably higher than with tetrasaccharide. Natural antibodies to tetrasaccharide possess the properties of heterologous antibodies, i.

Antibodies to morphine as indicators of chronic morphine intoxication and impaired immune reactivity.

Chronic morphine treatment of rats for 2 and 4 weeks led to an increase in morphine-binding cells in the spleen, despite the general reduction of mononuclear cell content in the spleen and thymus. Simultaneously, serum antibodies to morphine (AbM) in haemagglutination titres 1:20 and higher appeared in 50% and 80% of animals, respectively. Animals with high titres of AbM had much weaker immune response to a thymus-dependent antigen (sheep red blood cells).

[Mechanisms of activation and the role of lipid peroxidation in exacerbation of chronic pancreatitis].

It is indicated that in patients with chronic pancreatitis, lipid peroxidation is activated during disease exacerbation and is reduced in the course of the clinical recovery. Activation of lipid peroxidation is associated with the increasing functional activity of neutrophils stimulated in turn by high trypsin content in blood. In the authors' opinion, such a closed mechanism (trypsin-functional activity of neutrophils-lipid peroxidation-trypsin) determines the maintenance of chronic pancreatitis exacerbation.