Enhancing glioma treatment with 3D scaffolds laden with upconversion nanoparticles and temozolomide in orthotopic mouse model.

Targeted drug delivery for primary brain tumors, particularly gliomas, is currently a promising approach to reduce patient relapse rates. The use of substitutable scaffolds, which enable the sustained release of clinically relevant doses of anticancer medications, offers the potential to decrease the toxic burden on the patient's organism while also enhancing their quality of life and overall survival. Upconversion nanoparticles (UCNPs) are being actively explored as promising agents for detection and monitoring of tumor growth, and as therapeutic agents that can provide isolated therapeutic effects and enhance standard chemotherapy.

Mentoring: Shaping the professional identity of the academic internal medicine hospitalist.

Background: Burnout and lagging academic productivity are pressing challenges in hospital medicine, leading to stagnation and attrition. Mentoring shapes professional identity formation and enhances faculty vitality and retention, but has not been optimized among academic hospitalists.

Objectives: We sought to explore how mentoring impacts academic hospitalist professional identity and to elucidate barriers to mentoring in the field.

The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening.

Problems with the male reproductive system are of both medical and social significance. As a rule, spermatozoa and seminal plasma proteomes are investigated separately to assess sperm quality. The current study aimed to compare ejaculate proteomes with spermatozoa and seminal plasma protein profiles regarding the identification of proteins related to fertility scores.

Ribosomal, Telomere, and Mitochondrial Repeat Copy Number Variations in Female Genomes during Ovarian Stimulation and the Prediction of Fertilization Outcome: A Pilot Study.

Introduction: Individual risk assessment of assisted reproductive technologies is essential for personalized treatment strategies. Genetic and genomic indicators of the response to stress by cells could provide individual prognostic indicators for fertilization (IVF) success. Such indicators include the copy number of ribosomal genes (rDNA), which modulates the level of protein synthesis, and the abundance of mitochondrial DNA (mtDNA), which provides the cell with energy, while the content of telomere repeats (TRs) indicate the biological age.

Contribution of Chronic Sleep Deprivation to Age-Related Neurodegeneration in a Mouse Model of Familial Alzheimer's Disease (5xFAD).

Sleep-wake cycle disorders most often accompany the elderly and are frequently associated with the development of neurodegenerative processes, primarily Alzheimer's disease. Sleep disturbances can be diagnosed in patients with AD even before the onset of memory and cognitive impairment, and become more pronounced as the disease progresses. Therefore, the expansion of our knowledge of how sleep relates to AD pathogenesis needs to be addressed as soon as possible.

Box-Behnken assisted development and validation of high-performance liquid chromatography method for the simultaneous determination of doxorubicin and vorinostat in polymeric nanoparticles.

While histone deacetylase inhibitors, such as vorinostat, demonstrate a significant effect against hematological cancers, their application for solid tumor treatment is limited. However, there is strong evidence that combinatorial administration of vorinostat and genotoxic agents (e.g.

3D-printed hyaluronic acid hydrogel scaffolds impregnated with neurotrophic factors (BDNF, GDNF) for post-traumatic brain tissue reconstruction.

Brain tissue reconstruction posttraumatic injury remains a long-standing challenge in neurotransplantology, where a tissue-engineering construct (scaffold, SC) with specific biochemical properties is deemed the most essential building block. Such three-dimensional (3D) hydrogel scaffolds can be formed using brain-abundant endogenous hyaluronic acid modified with glycidyl methacrylate by employing our proprietary photopolymerisation technique. Herein, we produced 3D hyaluronic scaffolds impregnated with neurotrophic factors (BDNF, GDNF) possessing 600 kPa Young's moduli and 336% swelling ratios.

Far-Red Fluorescent Murine Glioma Model for Accurate Assessment of Brain Tumor Progression.

Glioma is the most common brain tumor, for which no significant improvement in life expectancy and quality of life is yet possible. The creation of stable fluorescent glioma cell lines is a promising tool for in-depth studies of the molecular mechanisms of glioma initialization and pathogenesis, as well as for the development of new anti-cancer strategies. Herein, a new fluorescent glioma GL261-kat cell line stably expressing a far-red fluorescent protein (TurboFP635; Katushka) was generated and characterized, and then validated in a mouse orthotopic glioma model.

Protocol for Increasing the Sensitivity of MS-Based Protein Detection in Human Chorionic Villi.

An important step in the proteomic analysis of missing proteins is the use of a wide range of tissues, optimal extraction, and the processing of protein material in order to ensure the highest sensitivity in downstream protein detection. This work describes a purification protocol for identifying low-abundance proteins in human chorionic villi using the proposed "1DE-gel concentration" method. This involves the removal of SDS in a short electrophoresis run in a stacking gel without protein separation.

Circulating cell-free DNA concentration and DNase I activity of peripheral blood plasma change in case of pregnancy with intrauterine growth restriction compared to normal pregnancy.

The level of apoptosis is increased during pregnancy. Dying cells emit DNA that remains in blood circulation and is known as cell-free DNA (cfDNA). The concentration of cfDNA can reflect the level of cell death.

[The choice of pancreaticojejunal anastomosis based on the performance experience of 200 operations of pancreaticoduodenal resection].

Analysis of the surgical treatment results in 200 patients, operated for obstructive diseases of the biliopancreatoduodenal zone, in whom pancreaticoduodenal resection (PDR) was performed in 1991 - 2013 yrs period, were presented. The choice of the pancreaticojejunoanastomosis (PJA) variant in PDR have depended on dimension of diameter of the pancreatic gland stump and the jejunum diameter, as well as from state of pancreatic parenchyma and duct. Application of differentiated approach to choice of the PJA variant have permitted to reduce the complications rate, including the sutures insufficiency.

[Relative binding activity of dydrogesterone with blood mononuclear cells progesterone receptors of women included in the IVF program].

It has been shown that specific binding of dydrogesterone with progesterone receptors in blood mononuclear cells of the patients included in the programs of in vitro fertilization, varies from 0 to 298%, thus at 38% of women dydrogesterone concedes to a progesterone in the competition for receptors of a progesterone, and 62%--exceed. The revealed variations of specific binding dydrogesterone with receptors of a progesterone are a reserve of increase of efficiency of auxiliary reproductive technology at the expense of individual selection of hormonal agents.

[The influence of intracysternal insulin administration on the rat temporal behaviour dynamics].

Rat behaviour in the open field and elevated plus-maze was analyzed in rats after intracysternal administration of 2.5, 25, 50 and 200 ng of insulin in 45 min, 24 hrs and on the 9th day after single injection. Dose-dependent changes in 45 min occurred in both behavioural tests: insulin in low doses (2.

[A system for implementation of steep massage in patients with musculoskeletal pain syndromes].

A system for implementation of steep massage in patients with musculoskeletal pain syndromes is described. The system provides effective medical care of patients with bearing disorders, scoliosis, osteochondrosis, and other diseases. The system uses steep massage and the effect on the internal receptors of muscles around the backbone.

[Regulatory mechanisms of erythropoiesis by leukocytes in inflammation].

On the model of carrageenan-induced acute aseptic peritonitis in rats with use of the inhibitors of some leukocytic products it is shown that lysosomal proteinases inhibit erythropoiesis activation in inflammation. Lipoxygenase metabolites stimulate erythropoiesis. Eicosanoids as a whole stimulate erytropoiesis still more.

[Vitamin E and its structural analogs in tuberculosis].

Efficiency of tocopherol, its analog with a shortened side chain as well as their quinons for tuberculosis was determined. All the studied compounds inhibited peroxide-formation processes in the liver homogenate and mitochondria. The vitamin E amount in the blood is considerably decreased in case of tuberculosis.

[A new method of cultivating Mycobacterium tuberculosis].

The possibility is shown of microcultivation of Mycobacteria tuberculosis on semipermeable cellophane membranes covering solid nutrients. The new method of cultivation may be used for enhanced microbiological diagnosis of tuberculosis, for determination of drug resistance of the tuberculous pathogen as well as for assessment of the biological properties of Mycobacteria tuberculosis in live unstained culture.

[Bacteriostatic activity of blood in patients with pulmonary tuberculosis during polychemotherapy].

Polychemotherapy comprising 4-6 antituberculosis drugs and prescribed either only daily, daily or given on an intermittent basis (every other day) or only on an intermittent basis was given for 4-8 months to 102 patients with destructive pulmonary tuberculosis (in those newly diagnosed, treated earlier and with a process recurrence). Four and, less frequently, three drugs were used per day. Bacteriostatic blood activity (BBA) was high in 90.

[Combined use of antitubercular drugs and metabolites of the tricarboxylic acid cycle in experimental tuberculosis].

The efficacy of a combined use of isoniazid together with polyzid prolonged tuberculostatic and tricarboxylic acid cycle metabolites was experimentally studied in guinea pigs. Mortality rates, pathomorphologic test data and the extent of tuberculous affection in the animals confirm a fairly high efficacy of prolonged polyzid in combination with succinate and malate as pathogenetic drugs.

[Investigation of pathogens in the foci of pulmonary and osteo-articular tuberculosis].

The tubercle bacilli detection rate was determined by direct bacterioscopy and the culture plate method immediately in the disease foci in 123 patients with pulmonary tuberculosis and 78 patients with tuberculosis of bones and joints. The culture plate method was shown to have significant advantages over bacterioscopy. However, in some cases with negative responses to the culture plate test, bacterioscopy was the only procedure that detected the pathogen in resected lung tissues.