Publications by authors named "Klervi Even-Desrumeaux"
Article Synopsis
- Compact single-domain antibodies (sdAbs) are much smaller and more efficient than traditional monoclonal antibodies (mAbs), offering benefits like high specificity and stability in biotechnological applications.
- Carcinoembryonic antigen (CEA) is a protein found in various cancers, and its detection on tumor cells can be enhanced using fluorescent dyes and semiconductor quantum dots (QDs), which are superior in brightness and stability.
- The study found that biotinylated anti-CEA sdAbs provide more sensitive detection of CEA on tumor cells compared to biotinylated mAbs, especially when combined with highly fluorescent SA-QDs, significantly improving detection capabilities.
Background: The isolation of recombinant antibody fragments from displayed libraries represents a powerful alternative to the generation of IgGs using hybridoma technology. The selected antibody fragments can then be easily engineered into (multi)-tagged constructs of variable mass and complexity as well as reconstituted into Camelidae IgG-like molecules when expressed fused to Fc domains. Nevertheless, all antibody constructs depend on an oxidizing environment for correct folding and consequently still belong to the proteins difficult to express in bacteria.
View Article and Find Full Text PDFArticle Synopsis
- Phage display is a method to find binders for various antigens, which can be tested both on purified proteins and whole cells, but its efficiency can be inconsistent due to challenges in selection steps.* -
- A new technique called masked selection improves the process by blocking unwanted binding sites, allowing researchers to focus on relevant targets, demonstrated by successfully isolating antibodies against certain proteins and cancer markers.* -
- This approach was validated by identifying specific breast cancer markers and can be utilized for diverse types of antibody fragments, making it beneficial for swiftly discovering important cell surface markers.*
Article Synopsis
- Affinity determination is important for characterizing antibodies, and this text presents a method using flow cytometry for this purpose.* -
- The technique utilizes the strong bond between biotin and streptavidin for easy and efficient labeling of antibodies, with various labeling methods discussed.* -
- The document provides a detailed procedure for determining the dissociation constant (K[D]) using flow cytometry, outlining both chemical and enzymatic biotinylation techniques.*
Article Synopsis
- Traditional phage display methods involve attaching purified antigens to plates, but these don't work well for antigens on cell surfaces or unknown targets.
- A new panning strategy is introduced that includes a depletion step to target cell surface receptors directly.
- The protocol detailed includes steps for producing a phage library, selection and elution, screening through ELISA, and analyzing soluble antibodies, with variations provided later in the text.
Article Synopsis
- - Noninvasive detection of breast cancer is critical for improving patient outcomes, as it helps identify recurrence risks early based on disease stage at surgery, but identifying suitable biomarkers and monoclonal antibodies remains challenging.
- - Camelids provide unique single-domain antibodies (sdAbs) that are advantageous for cancer diagnostics due to their small size, stability, and high expression yield, making them ideal for various biotechnological applications.
- - Researchers created phage display libraries using breast cancer biopsies which led to the isolation of sdAbs targeting known antigens like HER2 and novel targets like cytokeratin 19, demonstrating a powerful method for developing effective diagnostic tools.
Unlabelled: Common strategy for diagnostics with quantum dots (QDs) utilizes the specificity of monoclonal antibodies (mAbs) for targeting. However QD-mAbs conjugates are not always well-suited for this purpose because of their large size. Here, we engineered ultrasmall nanoprobes through oriented conjugation of QDs with 13-kDa single-domain antibodies (sdAbs) derived from llama IgG.
View Article and Find Full Text PDFArticle Synopsis
- - Our understanding of tumor immunology has led to various cancer treatment strategies, but there is a lack of effective tumor markers for immunotherapy.
- - The focus is now on finding new targets for both active and passive immunotherapy to improve treatment options.
- - This review discusses recent progress in identifying biomarkers and tumor antigens, emphasizing the latest discovery methods such as "omics" technology.
Article Synopsis
- * Camelids produce unique antibodies (HCAbs) that form single domain antibody fragments (sdAbs), which are small, stable, and have high solubility suitable for various biotechnological applications.
- * This study developed antibody microarrays using sdAbs from bacterial lysates, testing different strategies for immobilization to enhance sensitivity and specificity, aiming for high-density arrays to improve proteomics and biomarker identification.
Understanding cellular systems requires identification and analysis of their multiple components and determination of how they act together and are regulated. Microarray technology is one of the few tools that is able to solve such problems. It is based on high-throughput recognition of a target to the probe and has the potential to simultaneously measure the presence of numerous molecules in multiplexed tests, all contained in a small drop of test fluid.
View Article and Find Full Text PDF