Comparison and partial characterization of the protein profiles and outer membrane antigens of Actinobacillus species isolated from ram lambs with epididymitis.

Three monoclonal antibodies (LG17, LG30, LG33) were used in the indirect fluorescent antibody test, the ELISA, and the immunoelectrotransfer blot technique to identify group-specific and strain-specific epitopes on the outer membranes of Actinobacillus seminis, A actinomycetemcomitans, and 17 field isolates of Actinobacillus spp. The field isolates had been obtained by bacteriologic culture of specimens from ram lambs with epididymitis. Only antibody LG33 consistently had specificity for an outer membrane epitope shared by most of the bacterial isolates tested.

Use of monoclonal antibodies to identify outer membrane antigens of Actinobacillus species.

Three monoclonal antibodies (LG17, LG30, and LG33) were used to identify outer membrane antigens of Actinobacillus sp (As8C isolate) cultured from the epididymides of an infected ram lamb. Specificity of the 3 antibodies to As8C antigens was determined by use of bacterial agglutination, the enzyme-linked immunosorbent assay, and the indirect fluorescent antibody test. Results of immunoelectron microscopy confirmed that each antibody was specific for epitopes on As8C outer membrane antigens.

Cytotoxicity of citrinin in cultured kidney epithelial cell systems.

Cytotoxicity of citrinin, a fungal metabolite and a common food contaminant, was evaluated in an established cell line, Madin-Darby bovine kidney (MDBK) cells and primary fetal bovine kidney (PFBK) cells. Citrinin is a known nephrotoxicant but produced a low order of cytotoxicity in cultured renal cells. A dose-dependent cytotoxic effect was observed at millimolar concentrations of the toxin.

Cellular uptake and subcellular distribution of mercury in chick embryonic retinal cell aggregates.

Cellular uptake and subcellular distribution of mercury (203HgCl2) were determined in the chick embryonic retinal cell aggregation system. The accumulation of mercury was dependent upon its concentration in the medium. The uptake was rapid; a maximum deposition of mercury at 5 microM occurred within the first 30 min followed by a decline.

Production and preliminary characterization of monoclonal antibodies to Actinobacillus sp isolated from epididymitis lesions in a ram.

A panel of 55 monoclonal antibodies to an Actinobacillus sp isolate (As8C strain) cultured from the epididymides of an infected ram was produced. Cell lines producing 5 of these antibodies were cloned and expanded by hybridoma tumor production in Balb/c mice. An isotype profile revealed that 1 cloned antibody belonged to the immunoglobulin (Ig) M class and the 4 remaining antibodies belonged to the IgG class.

Cadmium accumulation and subcellular distribution in relation to cadmium chloride induced cytotoxicity in vitro.

A bovine kidney cell culture system was used to assess what relationship cadmium (Cd) uptake and subcellular distribution had to cadmium chloride induced cytotoxicity. Twenty-four hour incubation with 0.1-10 microM Cd elicited 0-90% cytotoxicity.

Immunoprophylaxis of an ocular solid malignant tumor in cattle.

Individual Hereford cows bearing benign precursor lesions of ocular squamous cell carcinoma were treated by intralesional injection of mycobacterial cell walls in an oil-in-water emulsion in an attempt to interrupt neoplastic progression. Thirty-one months after treatment, statistical analysis of data indicated that intralesional BCG cell wall vaccine can interrupt this process and provides effective immunoprophylactic prevention of malignant disease.

A cell aggregation model for the protective effect of selenium and vitamin E on methylmercury toxicity.

Histotypic aggregation of embryonic neural retinal cells was chosen as a test model to evaluate mercury toxicity. After 24 h rotational culture with methylmercury (CH3HgCl) at 4 microM, aggregation was completely inhibited. A dose-response relationship between concentrations of methylmercury and final sizes of aggregates was found.

Intratumoral BCG therapy of transplanted head and neck tumors in strain 2 guinea pigs.

The generally unfavorable prognosis associated with advanced squamous cell carcinoma in the head and neck region in humans led us to immunotherapeutic experiments with bacillus Calmette-Guerin (BCG) in inbred guinea pigs with solid growing and lymphogenous metastasizing tumors. The injection of live BCG or BCG cell wall preparation (CWP) into the planum buccale in the guinea pig led to a pronounced local inflammatory reaction. If live BCG or BCG CWP were injected into the planum buccale together with line 10 tumor cells, no growth of the tumor could be observed.

Detection of cell-surface antigens of bovine ocular squamous cell carcinoma.

A serologic study was conducted to identify surface antigens on cultured cells of bovine ocular squamous cell carcinoma. Sera from cattle with various stages of disease were tested for reactivity with surface antigens of cultured autologous and allogeneic cells. Radioiodine-labeled protein A assays were conducted to determine the presence of antibodies for tumor cells.

[Intratumor immunotherapy with BCG cell wall preparations: development of a new therapy approach for head-neck tumors].

The present study deals with the influence of intratumoral injections of BCG cell-wall preparations (CWP) on the development of malignant tumors in the head-neck region. Line 10 tumor cells in strain 2 guinea pigs were used in a first assay. It became evident that intratumoral BCG-CWP injection leads to total tumor regression, a situation which can also be achieved by radical surgery.

Efficacy of intratumorally administered mycobacterial cell walls in the treatment of cattle with ocular carcinoma.

Individual Hereford cows with naturally occurring ocular squamous cell carcinoma were treated by intralesional injections of mycobacterial cell walls in an oil-in-water emulsion. Six of 23 treated animals were alive (5 free of tumor and 1 with arrested disease) at a minimum of 2.5 years after treatment, as compared to 1 of 18 controls.

Anchorage-dependent culture of line 10 guinea-pig hepatoma cells.

A simple method for the anchorage-dependent culture of line 10 guinea-pig hepatoma cells is described.

[Tumor models in animals for the elaboration of oncologic problems].

Three groups of tumor systems in animals must be differentiated: 1) xenogeneic, 2) allogeneic, 3) syngeneic systems. While the tumor is not genetically identical with the tumor host in xenogeneic and allogeneic systems, syngeneic models are distinguished by a homogeneous genetic background of tumor and host organism. Xenogeneic, allogeneic, and syngeneic inbred animal models, on the one hand, and spontaneously tumor development in outbred animals on the other, were introduced with which oncologic questions can be handled.

Cryopreservation of bovine mononuclear leukocytes.

A technique of cryopreservation of bovine mononuclear leukocytes for use in lymphocyte stimulation tests and cell identification studies has been developed. Dimethyl sulfoxide was used as the cryopreservation agent. Cells were frozen to --40 C at a controlled rate of --1 C/minute and then to --80 C at a rate of --4 C/minute, and were subsequently stored in liquid nitrogen (--109 C).

Regression of bovine ocular carcinoma by treatment with a mycobacterial vaccine.

Hereford cows with naturally occurring ocular squamous cell carcinoma were treated by injection of BCG cell-wall vaccine into the tumor. Regression or arrest of disease was observed in 71% of treated animals. The disease progressed in all untreated animals and animals treated with improperly compounded vaccine.