Medium engineering of phenylethanoid transfructosylation catalysed by yeast β-fructofuranosidase.

Tyrosol and hydroxytyrosol, by-products of olive oil production, are valuable substrates for enzymatic transglycosylation that can provide products with pharmaceutical potential. Phenylethanoid fructosides are produced from sucrose and phenylethanoids by the catalytic action of β-fructofuranosidases. This work dealt with the potential of the most abundant β-fructofuranosidase, baker's yeast invertase, for this bioconversion.

Screening of Commercial Enzymes for Transfructosylation of Tyrosol: Effect of Process Conditions and Reaction Network.

Enzymatic fructosylation of organic acceptors other than saccharides brings new possibilities to synthesize molecules that do not exist in nature. The introduction of fructosyl moiety may lead to glycosides possessing enhanced physicochemical and bioactive properties which could be useful in the pharmaceutical and cosmetic industry. In this work, the regioselective synthesis of tyrosol β-d-fructofuranoside (TF) catalyzed by β-fructofuranosidase is investigated.